Magnetic bead technology for viral RNA extraction from serum in blood bank screening
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/6694 http://dx.doi.org/10.1590/S1413-86702011000600008 |
Resumo: | Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. |
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Albertoni, Guilherme Ambrozio [UNIFESP]Arnoni, Carine PriscoAraujo, Patricia Regina BarbozaAndrade, Sheila Siqueira [UNIFESP]Carvalho, Fabrício OliveiraGirão, Manoel João Batista Castello [UNIFESP]Schor, Nestor [UNIFESP]Barreto, José AugustoAssociação Beneficente de Coleta de SangueUniversidade Federal de São Paulo (UNIFESP)2015-06-14T13:43:20Z2015-06-14T13:43:20Z2011-12-01Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 6, p. 547-552, 2011.1413-8670http://repositorio.unifesp.br/handle/11600/6694http://dx.doi.org/10.1590/S1413-86702011000600008S1413-86702011000600008.pdfS1413-8670201100060000810.1590/S1413-86702011000600008Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Associação Beneficente de Coleta de SangueUniversidade Federal de São Paulo (UNIFESP)UNIFESP, EPMSciELO547-552engBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious DiseasesHCVHIVinfectionreverse transcriptase polymerase chain reactionblood banksMagnetic bead technology for viral RNA extraction from serum in blood bank screeninginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-86702011000600008.pdfapplication/pdf1135693${dspace.ui.url}/bitstream/11600/6694/1/S1413-86702011000600008.pdfbe6260d1e327ac8c613cdf39e9bbe8a8MD51open accessTEXTS1413-86702011000600008.pdf.txtS1413-86702011000600008.pdf.txtExtracted texttext/plain25828${dspace.ui.url}/bitstream/11600/6694/2/S1413-86702011000600008.pdf.txted8493a637b1d2cf053ad1b055b174bfMD52open access11600/66942022-07-08 10:28:56.114open accessoai:repositorio.unifesp.br:11600/6694Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:17:59.020572Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
spellingShingle |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening Albertoni, Guilherme Ambrozio [UNIFESP] HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
title_short |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_full |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_fullStr |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_full_unstemmed |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
title_sort |
Magnetic bead technology for viral RNA extraction from serum in blood bank screening |
author |
Albertoni, Guilherme Ambrozio [UNIFESP] |
author_facet |
Albertoni, Guilherme Ambrozio [UNIFESP] Arnoni, Carine Prisco Araujo, Patricia Regina Barboza Andrade, Sheila Siqueira [UNIFESP] Carvalho, Fabrício Oliveira Girão, Manoel João Batista Castello [UNIFESP] Schor, Nestor [UNIFESP] Barreto, José Augusto |
author_role |
author |
author2 |
Arnoni, Carine Prisco Araujo, Patricia Regina Barboza Andrade, Sheila Siqueira [UNIFESP] Carvalho, Fabrício Oliveira Girão, Manoel João Batista Castello [UNIFESP] Schor, Nestor [UNIFESP] Barreto, José Augusto |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Associação Beneficente de Coleta de Sangue Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Albertoni, Guilherme Ambrozio [UNIFESP] Arnoni, Carine Prisco Araujo, Patricia Regina Barboza Andrade, Sheila Siqueira [UNIFESP] Carvalho, Fabrício Oliveira Girão, Manoel João Batista Castello [UNIFESP] Schor, Nestor [UNIFESP] Barreto, José Augusto |
dc.subject.eng.fl_str_mv |
HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
topic |
HCV HIV infection reverse transcriptase polymerase chain reaction blood banks |
description |
Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR. RESULTS: Out of 20.808 samples screened, 53 samples (29 for HCV and 24 for HIV) were confirmed as positive by serological and NAT methods. CONCLUSION: The manual magnetic bead-based extraction in combination with real-time PCR detection can be used to routinely screen blood donation for viremic donors to further increase the safety of blood products. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-12-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:43:20Z |
dc.date.available.fl_str_mv |
2015-06-14T13:43:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 6, p. 547-552, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/6694 http://dx.doi.org/10.1590/S1413-86702011000600008 |
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1413-8670 |
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S1413-86702011000600008.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702011000600008 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1413-86702011000600008 |
identifier_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 15, n. 6, p. 547-552, 2011. 1413-8670 S1413-86702011000600008.pdf S1413-86702011000600008 10.1590/S1413-86702011000600008 |
url |
http://repositorio.unifesp.br/handle/11600/6694 http://dx.doi.org/10.1590/S1413-86702011000600008 |
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eng |
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Brazilian Journal of Infectious Diseases |
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Brazilian Society of Infectious Diseases |
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Brazilian Society of Infectious Diseases |
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