Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection

Detalhes bibliográficos
Autor(a) principal: Kubota, Lauro Tatsuo, 1964-
Data de Publicação: 2022
Tipo de documento: Artigo
Título da fonte: Repositório da Produção Científica e Intelectual da Unicamp
Texto Completo: https://hdl.handle.net/20.500.12733/6720
Resumo: Agradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5)
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spelling Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detectionLeishmanioseDoenças negligenciadasLeishmaniasisNeglected diseasesPartial least squares discriminant analysisSERSImmunogenic peptidePromastigote surface antigenArtigo originalAgradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5)Abstract: The fast and reliable anti-leishmaniasis antibody detection method was developed using citrate-capped gold nanoparticles (AuNPs) conjugated with an immunogenic peptide from promastigote surface antigens. These conjugates recognize specific antibodies that promote the aggregation of AuNPs in the solution, enabling a remarkable surface-enhanced Raman scattering (SERS) activity which allowed the development of an indirect detection test for human visceral leishmaniasis (VL). Dynamic and static light scattering methods were performed to investigate the aggregation process of the peptide-capped AuNPs induced by the addition of the specific polyclonal antibody anti-PSAdP23–47 in the diagnostic test. UV–vis spectroscopy and scanning electron microscopy demonstrated that AuNPs of different sizes are formed after interacting with antibodies against promastigote surface antigen (PSA)-derived peptides from the amino acid residues 23–47 (PSAdP23–47), confirmed by the plasmon resonance band position from 536 to 639 nm and light-scattering techniques. Experimental optimization of the SERS-based serological test showed that increasing the peptide and the citrate buffer concentration induces stronger Raman intensities. In addition, the multivariate curve resolution with alternating least squares allowed the identification of a fingerprint window within the range of 1043–1498 cm–1. Finally, a refined data analysis approach was successfully employed, enabling a robust differentiation between human sera from VL and non-VL patients. Prototype validation and proof of concept to demonstrate the feasibility and functionality of the sensor were carried out through calibration and optimization using a partial least squares discriminant analysis model with six latent variables, which validated the test reaching 100% sensitivity and 88.2% specificity. These results show the potential of the SERS platform for constructing a point-of-need serological test for diagnosing VL, a neglected tropical disease with a strong humoral immune responseFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFechadoUNIVERSIDADE ESTADUAL DE CAMPINASKubota, Lauro Tatsuo, 1964-2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/6720KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1254652reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2022-12-06T13:29:58Zoai:https://www.repositorio.unicamp.br/:1254652Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2022-12-06T13:29:58Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
title Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
spellingShingle Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
Kubota, Lauro Tatsuo, 1964-
Leishmaniose
Doenças negligenciadas
Leishmaniasis
Neglected diseases
Partial least squares discriminant analysis
SERS
Immunogenic peptide
Promastigote surface antigen
Artigo original
title_short Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
title_full Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
title_fullStr Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
title_full_unstemmed Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
title_sort Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
author Kubota, Lauro Tatsuo, 1964-
author_facet Kubota, Lauro Tatsuo, 1964-
author_role author
dc.contributor.none.fl_str_mv UNIVERSIDADE ESTADUAL DE CAMPINAS
dc.contributor.author.fl_str_mv Kubota, Lauro Tatsuo, 1964-
dc.subject.por.fl_str_mv Leishmaniose
Doenças negligenciadas
Leishmaniasis
Neglected diseases
Partial least squares discriminant analysis
SERS
Immunogenic peptide
Promastigote surface antigen
Artigo original
topic Leishmaniose
Doenças negligenciadas
Leishmaniasis
Neglected diseases
Partial least squares discriminant analysis
SERS
Immunogenic peptide
Promastigote surface antigen
Artigo original
description Agradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5)
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/20.500.12733/6720
KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023.
url https://hdl.handle.net/20.500.12733/6720
identifier_str_mv KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023.
dc.language.iso.fl_str_mv Inglês
language_invalid_str_mv Inglês
dc.relation.none.fl_str_mv https://repositorio.unicamp.br/acervo/detalhe/1254652
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório da Produção Científica e Intelectual da Unicamp
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Repositório da Produção Científica e Intelectual da Unicamp
collection Repositório da Produção Científica e Intelectual da Unicamp
repository.name.fl_str_mv Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv reposip@unicamp.br
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