Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection
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Data de Publicação: | 2022 |
Tipo de documento: | Artigo |
Título da fonte: | Repositório da Produção Científica e Intelectual da Unicamp |
Texto Completo: | https://hdl.handle.net/20.500.12733/6720 |
Resumo: | Agradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5) |
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Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detectionLeishmanioseDoenças negligenciadasLeishmaniasisNeglected diseasesPartial least squares discriminant analysisSERSImmunogenic peptidePromastigote surface antigenArtigo originalAgradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5)Abstract: The fast and reliable anti-leishmaniasis antibody detection method was developed using citrate-capped gold nanoparticles (AuNPs) conjugated with an immunogenic peptide from promastigote surface antigens. These conjugates recognize specific antibodies that promote the aggregation of AuNPs in the solution, enabling a remarkable surface-enhanced Raman scattering (SERS) activity which allowed the development of an indirect detection test for human visceral leishmaniasis (VL). Dynamic and static light scattering methods were performed to investigate the aggregation process of the peptide-capped AuNPs induced by the addition of the specific polyclonal antibody anti-PSAdP23–47 in the diagnostic test. UV–vis spectroscopy and scanning electron microscopy demonstrated that AuNPs of different sizes are formed after interacting with antibodies against promastigote surface antigen (PSA)-derived peptides from the amino acid residues 23–47 (PSAdP23–47), confirmed by the plasmon resonance band position from 536 to 639 nm and light-scattering techniques. Experimental optimization of the SERS-based serological test showed that increasing the peptide and the citrate buffer concentration induces stronger Raman intensities. In addition, the multivariate curve resolution with alternating least squares allowed the identification of a fingerprint window within the range of 1043–1498 cm–1. Finally, a refined data analysis approach was successfully employed, enabling a robust differentiation between human sera from VL and non-VL patients. Prototype validation and proof of concept to demonstrate the feasibility and functionality of the sensor were carried out through calibration and optimization using a partial least squares discriminant analysis model with six latent variables, which validated the test reaching 100% sensitivity and 88.2% specificity. These results show the potential of the SERS platform for constructing a point-of-need serological test for diagnosing VL, a neglected tropical disease with a strong humoral immune responseFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFechadoUNIVERSIDADE ESTADUAL DE CAMPINASKubota, Lauro Tatsuo, 1964-2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12733/6720KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023.Inglêshttps://repositorio.unicamp.br/acervo/detalhe/1254652reponame:Repositório da Produção Científica e Intelectual da Unicampinstname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPinfo:eu-repo/semantics/openAccess2022-12-06T13:29:58Zoai:https://www.repositorio.unicamp.br/:1254652Repositório InstitucionalPUBhttp://repositorio.unicamp.br/oai/requestreposip@unicamp.bropendoar:2022-12-06T13:29:58Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
title |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
spellingShingle |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection Kubota, Lauro Tatsuo, 1964- Leishmaniose Doenças negligenciadas Leishmaniasis Neglected diseases Partial least squares discriminant analysis SERS Immunogenic peptide Promastigote surface antigen Artigo original |
title_short |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
title_full |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
title_fullStr |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
title_full_unstemmed |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
title_sort |
Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection |
author |
Kubota, Lauro Tatsuo, 1964- |
author_facet |
Kubota, Lauro Tatsuo, 1964- |
author_role |
author |
dc.contributor.none.fl_str_mv |
UNIVERSIDADE ESTADUAL DE CAMPINAS |
dc.contributor.author.fl_str_mv |
Kubota, Lauro Tatsuo, 1964- |
dc.subject.por.fl_str_mv |
Leishmaniose Doenças negligenciadas Leishmaniasis Neglected diseases Partial least squares discriminant analysis SERS Immunogenic peptide Promastigote surface antigen Artigo original |
topic |
Leishmaniose Doenças negligenciadas Leishmaniasis Neglected diseases Partial least squares discriminant analysis SERS Immunogenic peptide Promastigote surface antigen Artigo original |
description |
Agradecimentos: This work was supported by grants of FAPESP (grant no. 2017/02317-2), CNPq (grant no. 304389/2019-6), and the National Institute of Science and Technology in Bioanalytics (FAPESP grant no. 2014/50867-3 and CNPq grant no. 465389/2014-7). The authors are grateful to the Multiuser Central Facilities at UFABC. The authors are thankful to professor E.R.P. Filho for his experimental optimization insights. The authors are also indebted to professor M.L.A. Temperini from the Laboratory of Molecular Spectroscopy of the Institute of Chemistry of University of São Paulo (USP, Brazil), where SERS measurements were performed (FAPESP, grant 2016/21070-5) |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/20.500.12733/6720 KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023. |
url |
https://hdl.handle.net/20.500.12733/6720 |
identifier_str_mv |
KUBOTA, Lauro Tatsuo. Development and validation of a SERS-Based serological test combined with PLS-DA method for leishmaniasis detection. ACS applied electronic materials. Washington, DC : American Chemical Society, 2022. Vol. 4, n. 8 (Aug., 2022), p. 3997–4006. Disponível em: https://hdl.handle.net/20.500.12733/6720. Acesso em: 24 mai. 2023. |
dc.language.iso.fl_str_mv |
Inglês |
language_invalid_str_mv |
Inglês |
dc.relation.none.fl_str_mv |
https://repositorio.unicamp.br/acervo/detalhe/1254652 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório da Produção Científica e Intelectual da Unicamp instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
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Universidade Estadual de Campinas (UNICAMP) |
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UNICAMP |
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UNICAMP |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp |
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Repositório da Produção Científica e Intelectual da Unicamp - Universidade Estadual de Campinas (UNICAMP) |
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reposip@unicamp.br |
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