Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Crop Breeding and Applied Biotechnology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300191 |
Resumo: | Abstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemented with 1.34 μM NAA and 2.27 μM TDZ resulted in better response on PBLs, and subsequent shoot differentiation (55.25 shoots per explant), and in better rooting number and root length responses, favoring acclimatization with 90% of survived plants. However, the medium supplemented with only NAA (1.34 μM) resulted in 33.50 shoots per explant. Histological sections confirmed that only one PLB was induced per responsive root tip, and it showed numerous dispersed and extended meristemoids, or division centers that originated new PBLs. Additionally, this protocol could be an excellent model to study molecular aspects of root to shoot conversion. |
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Crop Breeding and Applied Biotechnology |
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Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explantsProtocorm-like bodiesthidiazuronα-naphthaleneacetic acidgerminationAbstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemented with 1.34 μM NAA and 2.27 μM TDZ resulted in better response on PBLs, and subsequent shoot differentiation (55.25 shoots per explant), and in better rooting number and root length responses, favoring acclimatization with 90% of survived plants. However, the medium supplemented with only NAA (1.34 μM) resulted in 33.50 shoots per explant. Histological sections confirmed that only one PLB was induced per responsive root tip, and it showed numerous dispersed and extended meristemoids, or division centers that originated new PBLs. Additionally, this protocol could be an excellent model to study molecular aspects of root to shoot conversion.Crop Breeding and Applied Biotechnology2017-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300191Crop Breeding and Applied Biotechnology v.17 n.3 2017reponame:Crop Breeding and Applied Biotechnologyinstname:Sociedade Brasileira de Melhoramento de Plantasinstacron:CBAB10.1590/1984-70332017v17n3a30info:eu-repo/semantics/openAccessPicolotto,Dayana Rotili NunesPaiva Neto,Vespasiano Borges deBarros,Fábio dePadilha,Daly Roxana CastroCruz,Ana Cláudia Ferreira daOtoni,Wagner Camposeng2017-07-21T00:00:00Zoai:scielo:S1984-70332017000300191Revistahttps://cbab.sbmp.org.br/#ONGhttps://old.scielo.br/oai/scielo-oai.phpcbabjournal@gmail.com||cbab@ufv.br1984-70331518-7853opendoar:2017-07-21T00:00Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantasfalse |
dc.title.none.fl_str_mv |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
title |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
spellingShingle |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants Picolotto,Dayana Rotili Nunes Protocorm-like bodies thidiazuron α-naphthaleneacetic acid germination |
title_short |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
title_full |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
title_fullStr |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
title_full_unstemmed |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
title_sort |
Micropropagation of Cyrtopodium paludicolum (Orchidaceae) from root tip explants |
author |
Picolotto,Dayana Rotili Nunes |
author_facet |
Picolotto,Dayana Rotili Nunes Paiva Neto,Vespasiano Borges de Barros,Fábio de Padilha,Daly Roxana Castro Cruz,Ana Cláudia Ferreira da Otoni,Wagner Campos |
author_role |
author |
author2 |
Paiva Neto,Vespasiano Borges de Barros,Fábio de Padilha,Daly Roxana Castro Cruz,Ana Cláudia Ferreira da Otoni,Wagner Campos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Picolotto,Dayana Rotili Nunes Paiva Neto,Vespasiano Borges de Barros,Fábio de Padilha,Daly Roxana Castro Cruz,Ana Cláudia Ferreira da Otoni,Wagner Campos |
dc.subject.por.fl_str_mv |
Protocorm-like bodies thidiazuron α-naphthaleneacetic acid germination |
topic |
Protocorm-like bodies thidiazuron α-naphthaleneacetic acid germination |
description |
Abstract An efficient protocol for in vitro plant propagation of Cyrtopodium paludicolum has been developed using root tips dissected from well-developed seedlings. Root tips were cultured on Knudson medium supplemented with α-naphthaleneacetic acid (NAA), and/or thidiazuron (TDZ). TDZ did not induce protocorm-like bodies (PLBs) in the NAA absence, indicating phytoregulators synergistic effect. Medium supplemented with 1.34 μM NAA and 2.27 μM TDZ resulted in better response on PBLs, and subsequent shoot differentiation (55.25 shoots per explant), and in better rooting number and root length responses, favoring acclimatization with 90% of survived plants. However, the medium supplemented with only NAA (1.34 μM) resulted in 33.50 shoots per explant. Histological sections confirmed that only one PLB was induced per responsive root tip, and it showed numerous dispersed and extended meristemoids, or division centers that originated new PBLs. Additionally, this protocol could be an excellent model to study molecular aspects of root to shoot conversion. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300191 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332017000300191 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1984-70332017v17n3a30 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
publisher.none.fl_str_mv |
Crop Breeding and Applied Biotechnology |
dc.source.none.fl_str_mv |
Crop Breeding and Applied Biotechnology v.17 n.3 2017 reponame:Crop Breeding and Applied Biotechnology instname:Sociedade Brasileira de Melhoramento de Plantas instacron:CBAB |
instname_str |
Sociedade Brasileira de Melhoramento de Plantas |
instacron_str |
CBAB |
institution |
CBAB |
reponame_str |
Crop Breeding and Applied Biotechnology |
collection |
Crop Breeding and Applied Biotechnology |
repository.name.fl_str_mv |
Crop Breeding and Applied Biotechnology - Sociedade Brasileira de Melhoramento de Plantas |
repository.mail.fl_str_mv |
cbabjournal@gmail.com||cbab@ufv.br |
_version_ |
1754209187208888320 |