Micropropagation of Brasilidium forbesii (Orchidaceae) through transverse and longitudinal thin cell layer culture

• Main Author: Gomes, Lucas Roberto Pereira
• Publication Date: 2015
• Other Authors: Franceschi, Cristina do Rosário Batista, Ribas, Luciana Lopes Fortes
• Format: Article
• Language: eng
• Source: Acta Scientiarum Biological Sciences
• Download full: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/27276
• topic: epiphytic orchid; in vitro propagation; protocorms; protocorm-like body; 2.03.00.00-0

An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival).