Molecular characterization of 'sweet' cassavas (Manihot esculenta) from a germplasm bank in Brazilian Eastern Amazonia

• Autor(a) principal: Cunha,Elisa Ferreira Moura
• Data de Publicação: 2016
• Outros Autores: Silva,Carlos Rogério de Sousa, Albuquerque,Paulo Sérgio Bevilaqua de, Ramalho,Girena Fernandes, Pontes,Lígia Cristine Gonçalves, Farias Neto,João Tomé de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Crop Breeding and Applied Biotechnology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332016000100028
• topic: Genetic resources; microsatellite markers; Euphorbiaceae; duplicates

Abstract Genetic variability of a set of 81 accessions of 'sweet' cassavas (Manihot esculenta) collected mostly in the North region of Brazil was investigated with nine microsatellite loci. All loci were polymorphic, with mean of 6.33 alleles per locus. Analyses indicated that 35 multiloci profiles were represented by a single accession, and 46 showed non-unique profiles, represented by eleven genotypes. Forty-six different multiloci profiles were detected. Most of the putative duplicated accessions were collected in different locations. After the removal of putative duplicated genotypes, genetic parameters were estimated and expected heterozygosity was high (HE=0.73), indicating genetic variability. Structure analysis of this set of 'sweet' cassavas divided the 46 genotypes into two clusters (K=2), and a few genotypes had mixed ancestry. Results indicated the habit of exchange of materials among farmers of the North region of Brazil, and the genetic variability to be exploited in genetic breeding efforts.