Antifungal susceptibility testing of yeast isolated from corneal infections
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos brasileiros de oftalmologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492003000500019 |
Resumo: | PURPOSE: To report the antifungal susceptibility profile of yeast isolates obtained from cases of keratitis. METHODS: Susceptibility testing of 15 yeast strains isolated from corneal infections to amphotericin B, fluconazole, itraconazole and ketoconazole was performed using the NCCLS broth microdilution assay. RESULTS: Most episodes of eye infections were caused by Candida albicans. The antifungal drugs tested showed the following minimal inhibitory concentration values against yeast isolates: 0.125-0.5 µg/ml for amphotericin B; 0.125->64.0 µg/ml for fluconazole; 0.015-1.0 µg/ml for itraconazole and 0.015-0.125 µg/ml for ketoconazole. Despite the fact that all Candida isolates were judged to be susceptible to azoles, one isolate showed a minimal inhibitory concentration value significantly higher than a 90% minimal inhibitory concentration of all tested isolates. Rhodotorula rubra was resistant to fluconazole and itraconazole. CONCLUSIONS: Despite the fact that most yeast isolates from corneal infections are usually susceptible to amphotericin B and azoles, they exhibit a wide range of minimal inhibitory concentration values for antifungal drugs. The identification of strains at species level and their susceptibility pattern to antifungal drugs should be considered before determining the concentration to be used in topical antifungal formulations in order to optimize therapeutic response in eye infections. |
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Arquivos brasileiros de oftalmologia (Online) |
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Antifungal susceptibility testing of yeast isolated from corneal infectionsEye infectionsKeratitisAntifungal agentsYeasts infectionsAntifungal susceptibility testsPURPOSE: To report the antifungal susceptibility profile of yeast isolates obtained from cases of keratitis. METHODS: Susceptibility testing of 15 yeast strains isolated from corneal infections to amphotericin B, fluconazole, itraconazole and ketoconazole was performed using the NCCLS broth microdilution assay. RESULTS: Most episodes of eye infections were caused by Candida albicans. The antifungal drugs tested showed the following minimal inhibitory concentration values against yeast isolates: 0.125-0.5 µg/ml for amphotericin B; 0.125->64.0 µg/ml for fluconazole; 0.015-1.0 µg/ml for itraconazole and 0.015-0.125 µg/ml for ketoconazole. Despite the fact that all Candida isolates were judged to be susceptible to azoles, one isolate showed a minimal inhibitory concentration value significantly higher than a 90% minimal inhibitory concentration of all tested isolates. Rhodotorula rubra was resistant to fluconazole and itraconazole. CONCLUSIONS: Despite the fact that most yeast isolates from corneal infections are usually susceptible to amphotericin B and azoles, they exhibit a wide range of minimal inhibitory concentration values for antifungal drugs. The identification of strains at species level and their susceptibility pattern to antifungal drugs should be considered before determining the concentration to be used in topical antifungal formulations in order to optimize therapeutic response in eye infections.Conselho Brasileiro de Oftalmologia2003-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492003000500019Arquivos Brasileiros de Oftalmologia v.66 n.5 2003reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492003000500019info:eu-repo/semantics/openAccessMascaro,Vera Lucia Degaspare MonteHöfling-Lima,Ana LuisaGompertz,Olga FishmanYu,Maria Cecília ZoratMatta,Daniel Archimedes daColombo,Arnaldo Lopeseng2004-03-23T00:00:00Zoai:scielo:S0004-27492003000500019Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2004-03-23T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false |
dc.title.none.fl_str_mv |
Antifungal susceptibility testing of yeast isolated from corneal infections |
title |
Antifungal susceptibility testing of yeast isolated from corneal infections |
spellingShingle |
Antifungal susceptibility testing of yeast isolated from corneal infections Mascaro,Vera Lucia Degaspare Monte Eye infections Keratitis Antifungal agents Yeasts infections Antifungal susceptibility tests |
title_short |
Antifungal susceptibility testing of yeast isolated from corneal infections |
title_full |
Antifungal susceptibility testing of yeast isolated from corneal infections |
title_fullStr |
Antifungal susceptibility testing of yeast isolated from corneal infections |
title_full_unstemmed |
Antifungal susceptibility testing of yeast isolated from corneal infections |
title_sort |
Antifungal susceptibility testing of yeast isolated from corneal infections |
author |
Mascaro,Vera Lucia Degaspare Monte |
author_facet |
Mascaro,Vera Lucia Degaspare Monte Höfling-Lima,Ana Luisa Gompertz,Olga Fishman Yu,Maria Cecília Zorat Matta,Daniel Archimedes da Colombo,Arnaldo Lopes |
author_role |
author |
author2 |
Höfling-Lima,Ana Luisa Gompertz,Olga Fishman Yu,Maria Cecília Zorat Matta,Daniel Archimedes da Colombo,Arnaldo Lopes |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Mascaro,Vera Lucia Degaspare Monte Höfling-Lima,Ana Luisa Gompertz,Olga Fishman Yu,Maria Cecília Zorat Matta,Daniel Archimedes da Colombo,Arnaldo Lopes |
dc.subject.por.fl_str_mv |
Eye infections Keratitis Antifungal agents Yeasts infections Antifungal susceptibility tests |
topic |
Eye infections Keratitis Antifungal agents Yeasts infections Antifungal susceptibility tests |
description |
PURPOSE: To report the antifungal susceptibility profile of yeast isolates obtained from cases of keratitis. METHODS: Susceptibility testing of 15 yeast strains isolated from corneal infections to amphotericin B, fluconazole, itraconazole and ketoconazole was performed using the NCCLS broth microdilution assay. RESULTS: Most episodes of eye infections were caused by Candida albicans. The antifungal drugs tested showed the following minimal inhibitory concentration values against yeast isolates: 0.125-0.5 µg/ml for amphotericin B; 0.125->64.0 µg/ml for fluconazole; 0.015-1.0 µg/ml for itraconazole and 0.015-0.125 µg/ml for ketoconazole. Despite the fact that all Candida isolates were judged to be susceptible to azoles, one isolate showed a minimal inhibitory concentration value significantly higher than a 90% minimal inhibitory concentration of all tested isolates. Rhodotorula rubra was resistant to fluconazole and itraconazole. CONCLUSIONS: Despite the fact that most yeast isolates from corneal infections are usually susceptible to amphotericin B and azoles, they exhibit a wide range of minimal inhibitory concentration values for antifungal drugs. The identification of strains at species level and their susceptibility pattern to antifungal drugs should be considered before determining the concentration to be used in topical antifungal formulations in order to optimize therapeutic response in eye infections. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492003000500019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492003000500019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0004-27492003000500019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia v.66 n.5 2003 reponame:Arquivos brasileiros de oftalmologia (Online) instname:Conselho Brasileiro de Oftalmologia (CBO) instacron:CBO |
instname_str |
Conselho Brasileiro de Oftalmologia (CBO) |
instacron_str |
CBO |
institution |
CBO |
reponame_str |
Arquivos brasileiros de oftalmologia (Online) |
collection |
Arquivos brasileiros de oftalmologia (Online) |
repository.name.fl_str_mv |
Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO) |
repository.mail.fl_str_mv |
aboonline@cbo.com.br||abo@cbo.com.br |
_version_ |
1754209021688020992 |