Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-9946201759075 http://hdl.handle.net/11449/165931 |
Resumo: | The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) -33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria. |
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Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in BrazilUrine infectionsAntifungal agentsNosocomial candiduriaCandida speciesAntifungal susceptibilityThe aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) -33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria.FUNDECTUniv Fed Mato Grosso do Sul, Hosp Univ Maria Aparecida Pedrossian, Lab Anal Clin, Campo Grande, MS, BrazilUniv Fed Mato Grosso do Sul, Fac Ciencias Farmaceut Alimentos & Nutr, Campo Grande, MS USAUniv Sao Paulo, Inst Med Trop Sao Paulo, Lab Micol Med LIM 53, Sao Paulo, SP, BrazilUniv Estadual Paulista, Fac Med, Botucatu, SP, BrazilUniv Fed Mato Grosso do Sul, Fac Med, Ave Senador Fillinto,Muller,355, BR-79070900 Campo Grande, MS, BrazilUniv Estadual Paulista, Fac Med, Botucatu, SP, BrazilInst Medicina Tropical Sao PauloUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Espindola Lima, Glaucia MoreiraNunes, Maina de OliveiraChang, Marilene RodriguesSousa Tsujisaki, Rosianne Assis deNunes, Joslaine de OliveiraTaira, Cleison LedesmaThomaz, Danilo YamamotoBarbaro Del Negro, Gilda MariaMendes, Rinaldo Poncio [UNESP]Miranda Paniago, Anamaria Mello2018-11-29T05:07:16Z2018-11-29T05:07:16Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1590/S1678-9946201759075Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 59, 8 p., 2017.0036-4665http://hdl.handle.net/11449/16593110.1590/S1678-9946201759075S0036-46652017005000245WOS:000418613300001S0036-46652017005000245.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Do Instituto De Medicina Tropical De Sao Paulo0,669info:eu-repo/semantics/openAccess2023-10-29T06:08:40Zoai:repositorio.unesp.br:11449/165931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-29T06:08:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
title |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
spellingShingle |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil Espindola Lima, Glaucia Moreira Urine infections Antifungal agents Nosocomial candiduria Candida species Antifungal susceptibility |
title_short |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
title_full |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
title_fullStr |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
title_full_unstemmed |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
title_sort |
Identification and antifungal susceptibility of Candida species isolated from the urine of patients in a university hospital in Brazil |
author |
Espindola Lima, Glaucia Moreira |
author_facet |
Espindola Lima, Glaucia Moreira Nunes, Maina de Oliveira Chang, Marilene Rodrigues Sousa Tsujisaki, Rosianne Assis de Nunes, Joslaine de Oliveira Taira, Cleison Ledesma Thomaz, Danilo Yamamoto Barbaro Del Negro, Gilda Maria Mendes, Rinaldo Poncio [UNESP] Miranda Paniago, Anamaria Mello |
author_role |
author |
author2 |
Nunes, Maina de Oliveira Chang, Marilene Rodrigues Sousa Tsujisaki, Rosianne Assis de Nunes, Joslaine de Oliveira Taira, Cleison Ledesma Thomaz, Danilo Yamamoto Barbaro Del Negro, Gilda Maria Mendes, Rinaldo Poncio [UNESP] Miranda Paniago, Anamaria Mello |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Espindola Lima, Glaucia Moreira Nunes, Maina de Oliveira Chang, Marilene Rodrigues Sousa Tsujisaki, Rosianne Assis de Nunes, Joslaine de Oliveira Taira, Cleison Ledesma Thomaz, Danilo Yamamoto Barbaro Del Negro, Gilda Maria Mendes, Rinaldo Poncio [UNESP] Miranda Paniago, Anamaria Mello |
dc.subject.por.fl_str_mv |
Urine infections Antifungal agents Nosocomial candiduria Candida species Antifungal susceptibility |
topic |
Urine infections Antifungal agents Nosocomial candiduria Candida species Antifungal susceptibility |
description |
The aim of this study was to identify Candida spp. isolated from candiduria episodes at a tertiary hospital in the Midwest region of Brazil, and to determine their susceptibility profiles to antifungal compounds. From May 2011 to April 2012, Candida spp. isolated from 106 adult patients with candiduria admitted to the University Hospital of the Federal University of Mato Grosso do Sul were evaluated. Both, species identification and susceptibility testing with fluconazole-FLC, voriconazole-VRC, and amphotericin B-AmB were carried out using the Vitek 2. To discriminate species of the C. parapsilosis complex, a RAPD-PCR technique using the RPO2 primer was performed. From the total of 106 isolates, 42 (39.6%) C. albicans and 64 (60.4%) Candida non-albicans (CNA) -33 C. tropicalis, 18 C. glabrata, 5 C. krusei, 4 C. parapsilosis sensu stricto, 2 C. kefyr, 1 C. lusitaniae, and 1 C. guilliermondii were identified. All isolates were susceptible to AmB and VRC, whereas all C. glabrata isolates presented either resistance (5.6%) or dose-dependent susceptibility (94.4%) to FLC. The study of Candida spp. and their resistance profiles may help in tailoring more efficient therapeutic strategies for candiduria. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-11-29T05:07:16Z 2018-11-29T05:07:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-9946201759075 Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 59, 8 p., 2017. 0036-4665 http://hdl.handle.net/11449/165931 10.1590/S1678-9946201759075 S0036-46652017005000245 WOS:000418613300001 S0036-46652017005000245.pdf |
url |
http://dx.doi.org/10.1590/S1678-9946201759075 http://hdl.handle.net/11449/165931 |
identifier_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo: Inst Medicina Tropical Sao Paulo, v. 59, 8 p., 2017. 0036-4665 10.1590/S1678-9946201759075 S0036-46652017005000245 WOS:000418613300001 S0036-46652017005000245.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Do Instituto De Medicina Tropical De Sao Paulo 0,669 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
publisher.none.fl_str_mv |
Inst Medicina Tropical Sao Paulo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964745238839296 |