Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits

Detalhes bibliográficos
Autor(a) principal: Reggi,José Ricardo de Abreu
Data de Publicação: 2015
Outros Autores: Hida,Richard Yudi, Hida,Milton Massato, Nishiwaki-Dantas,Maria Cristina, Suzuki,Hisashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos brasileiros de oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492015000100003
Resumo: Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
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spelling Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbitsTissue plasminogen activatorAnterior chamberHyphemaInflammationPostoperative complicationsAnimalsRabbits Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin. Conselho Brasileiro de Oftalmologia2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492015000100003Arquivos Brasileiros de Oftalmologia v.78 n.1 2015reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.5935/0004-2749.20150003info:eu-repo/semantics/openAccessReggi,José Ricardo de AbreuHida,Richard YudiHida,Milton MassatoNishiwaki-Dantas,Maria CristinaSuzuki,Hisashieng2015-04-16T00:00:00Zoai:scielo:S0004-27492015000100003Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2015-04-16T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false
dc.title.none.fl_str_mv Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
spellingShingle Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
Reggi,José Ricardo de Abreu
Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
title_short Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_fullStr Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full_unstemmed Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_sort Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
author Reggi,José Ricardo de Abreu
author_facet Reggi,José Ricardo de Abreu
Hida,Richard Yudi
Hida,Milton Massato
Nishiwaki-Dantas,Maria Cristina
Suzuki,Hisashi
author_role author
author2 Hida,Richard Yudi
Hida,Milton Massato
Nishiwaki-Dantas,Maria Cristina
Suzuki,Hisashi
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reggi,José Ricardo de Abreu
Hida,Richard Yudi
Hida,Milton Massato
Nishiwaki-Dantas,Maria Cristina
Suzuki,Hisashi
dc.subject.por.fl_str_mv Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
topic Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
description Purpose: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492015000100003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492015000100003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/0004-2749.20150003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv Arquivos Brasileiros de Oftalmologia v.78 n.1 2015
reponame:Arquivos brasileiros de oftalmologia (Online)
instname:Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
instname_str Conselho Brasileiro de Oftalmologia (CBO)
instacron_str CBO
institution CBO
reponame_str Arquivos brasileiros de oftalmologia (Online)
collection Arquivos brasileiros de oftalmologia (Online)
repository.name.fl_str_mv Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)
repository.mail.fl_str_mv aboonline@cbo.com.br||abo@cbo.com.br
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