Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits

Detalhes bibliográficos
Autor(a) principal: Reggi, José Ricardo de Abreu
Data de Publicação: 2015
Outros Autores: Hida, Richard Yudi, Hida, Milton Massato [UNESP], Nishiwaki-Dantas, Maria Cristina, Suzuki, Hisashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?pid=S0004-27492015000100003&script=sci_arttext
http://hdl.handle.net/11449/127306
Resumo: To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
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spelling Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbitsUso tópico e subconjuntival de ativador de plasminogênio tecidual recombinante em coelhosTissue plasminogen activatorAnterior chamberHyphemaInflammationPostoperative complicationsAnimalsRabbitsAtivador de plasminogênio tecidualCâmera anteriorHifemaComplicações pós-operatóriasAnimaisCoelhosTo quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.Quantificar produtos de degradação de fibrina (PDF) após uso tópico e subconjunctival de ativador de plasminogênio tecidual recombinante (r-TPA) em coelhos. Métodos: Formação de fibrina foi induzida na câmara anterior em 25 coelhos. Cinco coelhos foram submetidos a injeção intracameral de r-TPA (controle positivo). Dez coelhos foram submetidos a injeção subconjuntival de r-TPA e dez coelhos foram submetidos a instilação tópica de r-TPA. Amostras de humor aquoso foram coletados e uma análise quantitativa dos produtos de degradação de fibrina foi realizada. Resultados: Não foi observado diferença estatisticamente significativa na degradação de fibrina em nenhum dos momentos estudados quando comparados com o controle. Porém foi observado diferença estatisticamente significante na quantificação do produtos de degradação de fibrina no grupo controle e no grupo subconjuntival. Conclusão: Produtos de degradação de fibrina foi observado nas amostras do grupo subconjunctival, porém, provavelmente não foi suficiente para degradar a fibrin presente. r-TPA tópico não foi efetivo em absorver fibrina na câmara anterior.Santa Casa de Misericórdia de São Paulo Department of OphthalmologyUniversidade de São Paulo Department of OphthalmologyUniversidade Estadual Paulista Department of OphthalmologyConselho Brasileiro de OftalmologiaSanta Casa de Misericórdia de São PauloUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Reggi, José Ricardo de AbreuHida, Richard YudiHida, Milton Massato [UNESP]Nishiwaki-Dantas, Maria CristinaSuzuki, Hisashi2015-08-26T19:19:06Z2015-08-26T19:19:06Z2015-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6-9application/pdfhttp://www.scielo.br/scielo.php?pid=S0004-27492015000100003&script=sci_arttextArquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.0004-2749http://hdl.handle.net/11449/127306S0004-27492015000100003S0004-27492015000100003.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivos Brasileiros de Oftalmologia1.0260,518info:eu-repo/semantics/openAccess2023-11-21T06:14:45Zoai:repositorio.unesp.br:11449/127306Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-21T06:14:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
Uso tópico e subconjuntival de ativador de plasminogênio tecidual recombinante em coelhos
title Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
spellingShingle Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
Reggi, José Ricardo de Abreu
Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
Ativador de plasminogênio tecidual
Câmera anterior
Hifema
Complicações pós-operatórias
Animais
Coelhos
title_short Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_fullStr Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_full_unstemmed Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
title_sort Subconjunctival and topical application of recombinant tissue plasminogen activator in rabbits
author Reggi, José Ricardo de Abreu
author_facet Reggi, José Ricardo de Abreu
Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
author_role author
author2 Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Santa Casa de Misericórdia de São Paulo
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Reggi, José Ricardo de Abreu
Hida, Richard Yudi
Hida, Milton Massato [UNESP]
Nishiwaki-Dantas, Maria Cristina
Suzuki, Hisashi
dc.subject.por.fl_str_mv Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
Ativador de plasminogênio tecidual
Câmera anterior
Hifema
Complicações pós-operatórias
Animais
Coelhos
topic Tissue plasminogen activator
Anterior chamber
Hyphema
Inflammation
Postoperative complications
Animals
Rabbits
Ativador de plasminogênio tecidual
Câmera anterior
Hifema
Complicações pós-operatórias
Animais
Coelhos
description To quantify fibrin degradation products after topical and subconjunctival administration of recombinant tissue plasminogen activator in rabbits. Methods: Fibrin formation was induced in the anterior chamber in 25 rabbits. Subsequently, five rabbits received an injection of r-TPA (positive control) in the anterior chamber, another 10 received a subconjunctival injection of r-TPA, and the remaining 10 received instillations of topical r-TPA. Afterwards, samples of aqueous humor were collected and semi-quantitative analysis of fibrin degradation products (FDP) was performed. Results: No statistical differences were noted between the treatment and control groups at any time point. Fibrin degradation products semi-quantification showed statistical improvement in the control group and the subconjunctival group. Conclusion: Fibrin degradation products were observed in the anterior chamber after subconjunctival administration of r-TPA. However, it was probably not sufficient to cause fibrin degradation. Topical r-TPA did not effectively absorb anterior chamber fibrin.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-26T19:19:06Z
2015-08-26T19:19:06Z
2015-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?pid=S0004-27492015000100003&script=sci_arttext
Arquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.
0004-2749
http://hdl.handle.net/11449/127306
S0004-27492015000100003
S0004-27492015000100003.pdf
url http://www.scielo.br/scielo.php?pid=S0004-27492015000100003&script=sci_arttext
http://hdl.handle.net/11449/127306
identifier_str_mv Arquivos Brasileiros de Oftalmologia, v. 78, n. 1, p. 6-9, 2015.
0004-2749
S0004-27492015000100003
S0004-27492015000100003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivos Brasileiros de Oftalmologia
1.026
0,518
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6-9
application/pdf
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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