Cytokeratin expression in corneal dystrophies

Detalhes bibliográficos
Autor(a) principal: Coutinho,Anamaria Baptista
Data de Publicação: 2011
Outros Autores: Freitas,Denise de, Souza Filho,João Pessoa de, Corrêa,Zélia Maria S., Odashiro,Alexandre Nakao, Burnier Jr,Miguel N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos brasileiros de oftalmologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000200010
Resumo: PURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.
id CBO-2_de6bda8ecce742bc5dbd1d9d1fbc4669
oai_identifier_str oai:scielo:S0004-27492011000200010
network_acronym_str CBO-2
network_name_str Arquivos brasileiros de oftalmologia (Online)
repository_id_str
spelling Cytokeratin expression in corneal dystrophiesCorneal dystrophies, hereditaryKeratins/physiologyImmunohistochemistryCornea/pathologyPURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.Conselho Brasileiro de Oftalmologia2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000200010Arquivos Brasileiros de Oftalmologia v.74 n.2 2011reponame:Arquivos brasileiros de oftalmologia (Online)instname:Conselho Brasileiro de Oftalmologia (CBO)instacron:CBO10.1590/S0004-27492011000200010info:eu-repo/semantics/openAccessCoutinho,Anamaria BaptistaFreitas,Denise deSouza Filho,João Pessoa deCorrêa,Zélia Maria S.Odashiro,Alexandre NakaoBurnier Jr,Miguel N.eng2011-07-11T00:00:00Zoai:scielo:S0004-27492011000200010Revistahttp://aboonline.org.br/https://old.scielo.br/oai/scielo-oai.phpaboonline@cbo.com.br||abo@cbo.com.br1678-29250004-2749opendoar:2011-07-11T00:00Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)false
dc.title.none.fl_str_mv Cytokeratin expression in corneal dystrophies
title Cytokeratin expression in corneal dystrophies
spellingShingle Cytokeratin expression in corneal dystrophies
Coutinho,Anamaria Baptista
Corneal dystrophies, hereditary
Keratins/physiology
Immunohistochemistry
Cornea/pathology
title_short Cytokeratin expression in corneal dystrophies
title_full Cytokeratin expression in corneal dystrophies
title_fullStr Cytokeratin expression in corneal dystrophies
title_full_unstemmed Cytokeratin expression in corneal dystrophies
title_sort Cytokeratin expression in corneal dystrophies
author Coutinho,Anamaria Baptista
author_facet Coutinho,Anamaria Baptista
Freitas,Denise de
Souza Filho,João Pessoa de
Corrêa,Zélia Maria S.
Odashiro,Alexandre Nakao
Burnier Jr,Miguel N.
author_role author
author2 Freitas,Denise de
Souza Filho,João Pessoa de
Corrêa,Zélia Maria S.
Odashiro,Alexandre Nakao
Burnier Jr,Miguel N.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Coutinho,Anamaria Baptista
Freitas,Denise de
Souza Filho,João Pessoa de
Corrêa,Zélia Maria S.
Odashiro,Alexandre Nakao
Burnier Jr,Miguel N.
dc.subject.por.fl_str_mv Corneal dystrophies, hereditary
Keratins/physiology
Immunohistochemistry
Cornea/pathology
topic Corneal dystrophies, hereditary
Keratins/physiology
Immunohistochemistry
Cornea/pathology
description PURPOSE: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. METHODS: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on pa- raffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). RESULTS: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. CONCLUSION: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000200010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-27492011000200010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0004-27492011000200010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
publisher.none.fl_str_mv Conselho Brasileiro de Oftalmologia
dc.source.none.fl_str_mv Arquivos Brasileiros de Oftalmologia v.74 n.2 2011
reponame:Arquivos brasileiros de oftalmologia (Online)
instname:Conselho Brasileiro de Oftalmologia (CBO)
instacron:CBO
instname_str Conselho Brasileiro de Oftalmologia (CBO)
instacron_str CBO
institution CBO
reponame_str Arquivos brasileiros de oftalmologia (Online)
collection Arquivos brasileiros de oftalmologia (Online)
repository.name.fl_str_mv Arquivos brasileiros de oftalmologia (Online) - Conselho Brasileiro de Oftalmologia (CBO)
repository.mail.fl_str_mv aboonline@cbo.com.br||abo@cbo.com.br
_version_ 1754209026818703360

Registros relacionados