GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16195 |
Resumo: | University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA. |
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Vojnov, LaraMartins, Mauricio A.Almeida, Jorge R.Ende, ZacharyRakasz, Eva G.Reynolds, Matthew R.Leon, Enrique JWeisgrau, Kim L.Burwitz, Benjamin J.Folkvord, Joy M.Santana, Marlon G. Veloso deNeves, Patrícia C. CostaConnick, ElizabethSkinner, Pamela J.Gostick, EmmaO'Connor, David H.Wilson, Nancy A.Bonaldo, Myrna C.Galler, RicardoPrice, David A.Douek, Danny C.Watkins, David I.2016-10-11T17:08:00Z2016-10-11T17:08:00Z2011VOJNOV, Lara; et al. GagCM9-Specific CD8+ T Cells Expressing Limited Public TCR Clonotypes Do Not Suppress SIV Replication In Vivo. Plos One, v.6, n.8, e23515, 12p, Aug. 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/1619510.1371/journal.pone.0023515engPublic Library of ScienceReceptores de Antígenos de Linfócitos T gama-deltaCD8+ T cellsReplicação viralGagCM9-Specific CD8+ T CellsTCR ClonotypesSIV Replication In VivoGagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vaccine Research Center. Human Immunology Section. Bethesda, Maryland, USA.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vaccine Research Center. Human Immunology Section. Bethesda, Maryland, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA / Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.University of Colorado Denver School of Medicine.Denver, Colorado, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.University of Colorado Denver School of Medicine.Denver, Colorado, USA.University of Minnesota. Department of Veterinary and Biomedical Sciences. St. Paul, Minnesota, USA.Cardiff University. Department of Infection, Immunity and Biochemistry. Wales, United Kingdom.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Cardiff University. Department of Infection, Immunity and Biochemistry. Wales, United Kingdom.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Vaccine Research Center. Human Immunology Section. Bethesda, Maryland, USA.University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA / Wisconsin National Primate Research Center. Madison, Wisconsin, USA..Several lines of evidence suggest that HIV/SIV-specific CD8(+) T cells play a critical role in the control of viral replication. Recently we observed high levels of viremia in Indian rhesus macaques vaccinated with a segment of SIVmac239 Gag (Gag(45-269)) that were subsequently infected with SIVsmE660. These seven Mamu-A*01(+) animals developed CD8(+) T cell responses against an immunodominant epitope in Gag, GagCM9, yet failed to control virus replication. We carried out a series of immunological and virological assays to understand why these Gag-specific CD8(+) T cells could not control virus replication in vivo. GagCM9-specific CD8(+) T cells from all of the animals were multifunctional and were found in the colonic mucosa. Additionally, GagCM9-specific CD8(+) T cells accessed B cell follicles, the primary residence of SIV-infected cells in lymph nodes, with effector to target ratios between 20-250 GagCM9-specific CD8(+) T cells per SIV-producing cell. Interestingly, vaccinated animals had few public TCR clonotypes within the GagCM9-specific CD8(+) T cell population pre- and post-infection. The number of public TCR clonotypes expressed by GagCM9-specific CD8(+) T cells post-infection significantly inversely correlated with chronic phase viral load. It is possible that these seven animals failed to control viral replication because of the narrow TCR repertoire expressed by the GagCM9-specific CD8(+) T cell population elicited by vaccination and infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16195/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpatricia_neves_etal_etal_IOC_2011.pdfpatricia_neves_etal_etal_IOC_2011.pdfapplication/pdf2466795https://www.arca.fiocruz.br/bitstream/icict/16195/2/patricia_neves_etal_etal_IOC_2011.pdf9ce83dbb16e1f0cbf545954c7f988d91MD52TEXTpatricia_neves_etal_etal_IOC_2011.pdf.txtpatricia_neves_etal_etal_IOC_2011.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| title |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| spellingShingle |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo Vojnov, Lara Receptores de Antígenos de Linfócitos T gama-delta CD8+ T cells Replicação viral GagCM9-Specific CD8+ T Cells TCR Clonotypes SIV Replication In Vivo |
| title_short |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| title_full |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| title_fullStr |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| title_full_unstemmed |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| title_sort |
GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo |
| author |
Vojnov, Lara |
| author_facet |
Vojnov, Lara Martins, Mauricio A. Almeida, Jorge R. Ende, Zachary Rakasz, Eva G. Reynolds, Matthew R. Leon, Enrique J Weisgrau, Kim L. Burwitz, Benjamin J. Folkvord, Joy M. Santana, Marlon G. Veloso de Neves, Patrícia C. Costa Connick, Elizabeth Skinner, Pamela J. Gostick, Emma O'Connor, David H. Wilson, Nancy A. Bonaldo, Myrna C. Galler, Ricardo Price, David A. Douek, Danny C. Watkins, David I. |
| author_role |
author |
| author2 |
Martins, Mauricio A. Almeida, Jorge R. Ende, Zachary Rakasz, Eva G. Reynolds, Matthew R. Leon, Enrique J Weisgrau, Kim L. Burwitz, Benjamin J. Folkvord, Joy M. Santana, Marlon G. Veloso de Neves, Patrícia C. Costa Connick, Elizabeth Skinner, Pamela J. Gostick, Emma O'Connor, David H. Wilson, Nancy A. Bonaldo, Myrna C. Galler, Ricardo Price, David A. Douek, Danny C. Watkins, David I. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vojnov, Lara Martins, Mauricio A. Almeida, Jorge R. Ende, Zachary Rakasz, Eva G. Reynolds, Matthew R. Leon, Enrique J Weisgrau, Kim L. Burwitz, Benjamin J. Folkvord, Joy M. Santana, Marlon G. Veloso de Neves, Patrícia C. Costa Connick, Elizabeth Skinner, Pamela J. Gostick, Emma O'Connor, David H. Wilson, Nancy A. Bonaldo, Myrna C. Galler, Ricardo Price, David A. Douek, Danny C. Watkins, David I. |
| dc.subject.other.pt_BR.fl_str_mv |
Receptores de Antígenos de Linfócitos T gama-delta CD8+ T cells Replicação viral |
| topic |
Receptores de Antígenos de Linfócitos T gama-delta CD8+ T cells Replicação viral GagCM9-Specific CD8+ T Cells TCR Clonotypes SIV Replication In Vivo |
| dc.subject.en.pt_BR.fl_str_mv |
GagCM9-Specific CD8+ T Cells TCR Clonotypes SIV Replication In Vivo |
| description |
University of Wisconsin-Madison. Department of Pathology and Laboratory Medicine. Madison, WI, USA. |
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2011 |
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2011 |
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2016-10-11T17:08:00Z |
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2016-10-11T17:08:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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VOJNOV, Lara; et al. GagCM9-Specific CD8+ T Cells Expressing Limited Public TCR Clonotypes Do Not Suppress SIV Replication In Vivo. Plos One, v.6, n.8, e23515, 12p, Aug. 2011. |
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https://www.arca.fiocruz.br/handle/icict/16195 |
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1932-6203 |
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10.1371/journal.pone.0023515 |
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VOJNOV, Lara; et al. GagCM9-Specific CD8+ T Cells Expressing Limited Public TCR Clonotypes Do Not Suppress SIV Replication In Vivo. Plos One, v.6, n.8, e23515, 12p, Aug. 2011. 1932-6203 10.1371/journal.pone.0023515 |
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