Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization

Detalhes bibliográficos
Autor(a) principal: Britto, C.
Data de Publicação: 1995
Outros Autores: Cardoso, M. A., Ravel, C., Santoro, A., Pereira, J. Borges, Coura, José Rodrigues, Morel, Carlos Medicis, Winckler, P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14679
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
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spelling Britto, C.Cardoso, M. A.Ravel, C.Santoro, A.Pereira, J. BorgesCoura, José RodriguesMorel, Carlos MedicisWinckler, P.2016-06-29T16:36:10Z2016-07-07T12:15:21Z2016-06-29T16:36:10Z2016-07-07T12:15:21Z1995BRITTO, C. et al. Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization. Experimental Parasitology, v. 81, p. 462-471, 1995.0014-489410.1006/expr.1995.1139https://www.arca.fiocruz.br/handle/icict/146791090-2449engElsevierNordeste do BrasilTrypanosoma cruziChronic Chagasic PatientsNortheastern BrazilPCR diagnosisKinetoplast DNANonradiactive hybridizationTrypanosoma cruziDoença de ChagasDNA de CinetoplastoReação em Cadeia da PolimeraseTrypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Université de Montpellier. Faculte de Medicine. Laboratoire de Parasitologie. Montpellier, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Blood samples from 172 individuals from northeastern Brazil were subjected to PCR amplification of Trypanosoma cruzi-specific kDNA sequences. This method enabled us to detect parasite DNA in 21 of 47 patients that were serologically positive, In addition, 1 patient that gave doubtful results with chagasic serology was confirmed as positive by PCR. We applied the same PCR detection method to the feces of wild triatomines captured in the same region, obtaining three positive results that were confirmed by microscopic examination. The 25 amplified products obtained in this study were then reamplified with primers that gave a final amplicon containing sequences from the most variable region of kDNA minicircles. These were used as probes in hybridization experiments aimed at defining the degree of relatedness between the strains infecting humans and insects based on kDNA homologies. We found that the amplification products from the three triatomines were related and showed no crosshybridization with those obtained from human infections. Eight amplified products from human infections showed no cross-hybridization and did not hybridize with products from other patients. This indicates that the strains of T. cruzi circulating in the region present a high level of genetic heterogeneity. Finally, a number of amplified products hybridized with amplicons that did not hybridize with each other, indicating that infections with a parasite population presenting a mixed kDNA content (either due to different strains of T. cruzi or to a hybrid parasite) are a more frequent event than previously thought.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos21_morel_etal_IOC_1995.pdf.txtcarlos21_morel_etal_IOC_1995.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
title Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
spellingShingle Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
Britto, C.
Nordeste do Brasil
Trypanosoma cruzi
Chronic Chagasic Patients
Northeastern Brazil
PCR diagnosis
Kinetoplast DNA
Nonradiactive hybridization
Trypanosoma cruzi
Doença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
title_short Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
title_full Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
title_fullStr Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
title_full_unstemmed Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
title_sort Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization
author Britto, C.
author_facet Britto, C.
Cardoso, M. A.
Ravel, C.
Santoro, A.
Pereira, J. Borges
Coura, José Rodrigues
Morel, Carlos Medicis
Winckler, P.
author_role author
author2 Cardoso, M. A.
Ravel, C.
Santoro, A.
Pereira, J. Borges
Coura, José Rodrigues
Morel, Carlos Medicis
Winckler, P.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Britto, C.
Cardoso, M. A.
Ravel, C.
Santoro, A.
Pereira, J. Borges
Coura, José Rodrigues
Morel, Carlos Medicis
Winckler, P.
dc.subject.other.pt_BR.fl_str_mv Nordeste do Brasil
topic Nordeste do Brasil
Trypanosoma cruzi
Chronic Chagasic Patients
Northeastern Brazil
PCR diagnosis
Kinetoplast DNA
Nonradiactive hybridization
Trypanosoma cruzi
Doença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chronic Chagasic Patients
Northeastern Brazil
PCR diagnosis
Kinetoplast DNA
Nonradiactive hybridization
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
DNA de Cinetoplasto
Reação em Cadeia da Polimerase
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2016-06-29T16:36:10Z
2016-07-07T12:15:21Z
dc.date.available.fl_str_mv 2016-06-29T16:36:10Z
2016-07-07T12:15:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITTO, C. et al. Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization. Experimental Parasitology, v. 81, p. 462-471, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14679
dc.identifier.issn.pt_BR.fl_str_mv 0014-4894
10.1006/expr.1995.1139
dc.identifier.eissn.pt_BR.fl_str_mv 1090-2449
identifier_str_mv BRITTO, C. et al. Trypanosoma cruzi: parasite detection and strain classification in chronic chagasic patients from North-Eastern Brazil using PCR amplification of kinetoplast DNA and non-radioactive hybridization. Experimental Parasitology, v. 81, p. 462-471, 1995.
0014-4894
10.1006/expr.1995.1139
1090-2449
url https://www.arca.fiocruz.br/handle/icict/14679
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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