The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi

Alves, Ceres Luciana; Repolês, Bruno Marçal; Silva, Marcelo Santos da; Mendes, Isabela Cecília; Marin, Paula Andrea; Aguiar, Pedro Henrique Nascimento; Santos, Selma da Silva; Franco, Glória Regina; Macedo, Andréa Mara; Pena, Sérgio Danilo Junho; Andrade, Luciana de Oliveira; Guarneri, Alessandra Aparecida; Tahara, Erich Birelli; Elias, Maria Carolina; Machado, Carlos Renato

The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal:	Alves, Ceres Luciana
Data de Publicação:	2018
Outros Autores:	Repolês, Bruno Marçal, Silva, Marcelo Santos da, Mendes, Isabela Cecília, Marin, Paula Andrea, Aguiar, Pedro Henrique Nascimento, Santos, Selma da Silva, Franco, Glória Regina, Macedo, Andréa Mara, Pena, Sérgio Danilo Junho, Andrade, Luciana de Oliveira, Guarneri, Alessandra Aparecida, Tahara, Erich Birelli, Elias, Maria Carolina, Machado, Carlos Renato
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/33639
Resumo:	Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.

Metadados do item

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network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Alves, Ceres LucianaRepolês, Bruno MarçalSilva, Marcelo Santos daMendes, Isabela CecíliaMarin, Paula AndreaAguiar, Pedro Henrique NascimentoSantos, Selma da SilvaFranco, Glória ReginaMacedo, Andréa MaraPena, Sérgio Danilo JunhoAndrade, Luciana de OliveiraGuarneri, Alessandra AparecidaTahara, Erich BirelliElias, Maria CarolinaMachado, Carlos Renato2019-06-24T16:57:35Z2019-06-24T16:57:35Z2018ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3363910.1038/s41598-018-31541-zengNature ResearchTrypanosoma cruziTrypanosoma cruziThe recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG Brazil.Universidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular. Laboratório Especial de Ciclo Celular. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Detection of genetic exchange has been a limiting factor to deepen the knowledge on the mechanisms by which Trypanosoma cruzi is able to generate progeny and genetic diversity. Here we show that incorporation of halogenated thymidine analogues, followed by immunostaining, is a reliable method not only to detect T. cruzi fused-cell hybrids, but also to quantify their percentage in populations of this parasite. Through this approach, we were able to detect and quantify fused-cell hybrids of T. cruzi clones CL Brener and Y. Given the increased detection of fused-cell hybrids in naturally-occurring hybrid CL Brener strain, which displays increased levels of RAD51 and BRCA2 transcripts, we further investigated the role of Rad51 – a recombinase involved in homologous recombination – in the process of genetic exchange. We also verified that the detection of fused-cell hybrids in T. cruzi overexpressing RAD51 is increased when compared to wild-type cells, suggesting a key role for Rad51 either in the formation or in the stabilization of fused-cell hybrids in this organism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33639/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe recombinase Rad51.pdfThe recombinase Rad51.pdfapplication/pdf7462652https://www.arca.fiocruz.br/bitstream/icict/33639/2/The%20recombinase%20Rad51.pdfd2c0b2a73c5ee203c2681bcf6f90618eMD52TEXTThe recombinase Rad51.pdf.txtThe recombinase Rad51.pdf.txtExtracted texttext/plain559https://www.arca.fiocruz.br/bitstream/icict/33639/3/The%20recombinase%20Rad51.pdf.txtaa69fcf2f8ea54b70784b5737114c772MD53icict/336392019-06-25 21:40:16.737oai:www.arca.fiocruz.br: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Repositório 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dc.title.pt_BR.fl_str_mv	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
title	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
spellingShingle	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi Alves, Ceres Luciana Trypanosoma cruzi Trypanosoma cruzi
title_short	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
title_full	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
title_fullStr	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
title_full_unstemmed	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
title_sort	The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi
author	Alves, Ceres Luciana
author_facet	Alves, Ceres Luciana Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato
author_role	author
author2	Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato
author2_role	author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Alves, Ceres Luciana Repolês, Bruno Marçal Silva, Marcelo Santos da Mendes, Isabela Cecília Marin, Paula Andrea Aguiar, Pedro Henrique Nascimento Santos, Selma da Silva Franco, Glória Regina Macedo, Andréa Mara Pena, Sérgio Danilo Junho Andrade, Luciana de Oliveira Guarneri, Alessandra Aparecida Tahara, Erich Birelli Elias, Maria Carolina Machado, Carlos Renato
dc.subject.other.pt_BR.fl_str_mv	Trypanosoma cruzi
topic	Trypanosoma cruzi Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv	Trypanosoma cruzi
description	Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.
publishDate	2018
dc.date.issued.fl_str_mv	2018
dc.date.accessioned.fl_str_mv	2019-06-24T16:57:35Z
dc.date.available.fl_str_mv	2019-06-24T16:57:35Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/33639
dc.identifier.issn.pt_BR.fl_str_mv	2045-2322
dc.identifier.doi.none.fl_str_mv	10.1038/s41598-018-31541-z
identifier_str_mv	ALVES, Ceres Luciana et al. The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi. Scientific Reports, v. 8, p. 1-12, 2018. 2045-2322 10.1038/s41598-018-31541-z
url	https://www.arca.fiocruz.br/handle/icict/33639
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Nature Research
publisher.none.fl_str_mv	Nature Research
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/33639/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/33639/2/The%20recombinase%20Rad51.pdf https://www.arca.fiocruz.br/bitstream/icict/33639/3/The%20recombinase%20Rad51.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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The recombinase Rad51 plays a key role in events of genetic exchange in Trypanosoma cruzi

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