Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi

Detalhes bibliográficos
Autor(a) principal: Carrasco, Hernan J
Data de Publicação: 1996
Outros Autores: Frame, Iain A, Valente, Sebastião Aldo da Silva, Miles, Michael. A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Digital do Instituto Evandro Chagas (Patuá)
Texto Completo: https://patua.iec.gov.br/handle/iec/671
Resumo: Thirty six stocks of Trypanosoma cruzi isolated from sylvatic mammals (32 Didelphis marsupialis and one Philander opossum) and triatomine bugs (Rhodnius robustus and one unidentified bug) in the Amazonian forest of Carajas, Brazil were characterized by isoenzyme and random amplified polymorphic DNA (RAPD) analysis as belonging to principal zymodeme 1 (Zl). Two different homozygous phenotypes and the corresponding heterozygous phenotype were found for phosphoglucomutase with an observed frequency almost identical with that predicted by the theoretical Hardy- Weinberg distribution. Parental and hybrid profiles were algo suggested by RAPD analysis, which allowed exclusion of mixed parental strains from the hybrids: isoenzyme and RAPD profiles of biological clones were algo indistinguishable from those of uncloned stocks. Trypanosoma cruzi stocks from widely separated geographic origins in Central and South America gave similar RAPD profiles that allowed them to be recognized as being Zl. These results indicate that genetic exchange could contribute to the generation of genetic diversity during the sylvatic cycle of T. cruzi, and this may have epidemiologic and taxonomic implications.
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spelling Carrasco, Hernan JFrame, Iain AValente, Sebastião Aldo da SilvaMiles, Michael. A2016-01-26T11:36:39Z2016-01-26T11:36:39Z1996CARRASCO, Hernan J. et al. Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi. American Journal of Tropical Medicine and Hygiene, v. 54, n. 4, p. 418-424, 1996.0002-9637https://patua.iec.gov.br/handle/iec/671Thirty six stocks of Trypanosoma cruzi isolated from sylvatic mammals (32 Didelphis marsupialis and one Philander opossum) and triatomine bugs (Rhodnius robustus and one unidentified bug) in the Amazonian forest of Carajas, Brazil were characterized by isoenzyme and random amplified polymorphic DNA (RAPD) analysis as belonging to principal zymodeme 1 (Zl). Two different homozygous phenotypes and the corresponding heterozygous phenotype were found for phosphoglucomutase with an observed frequency almost identical with that predicted by the theoretical Hardy- Weinberg distribution. Parental and hybrid profiles were algo suggested by RAPD analysis, which allowed exclusion of mixed parental strains from the hybrids: isoenzyme and RAPD profiles of biological clones were algo indistinguishable from those of uncloned stocks. Trypanosoma cruzi stocks from widely separated geographic origins in Central and South America gave similar RAPD profiles that allowed them to be recognized as being Zl. These results indicate that genetic exchange could contribute to the generation of genetic diversity during the sylvatic cycle of T. cruzi, and this may have epidemiologic and taxonomic implications.London School of Hygiene and Tropical Medicine. Department of Medical Parasitology. London, UK.London School of Hygiene and Tropical Medicine. Department of Medical Parasitology. London, UK.Ministério da Saúde. Fundação Nacional de Saúde. Instituto Evandro Chagas. Belém, PA, Brasil.London School of Hygiene and Tropical Medicine. Department of Medical Parasitology. 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dc.title.pt_BR.fl_str_mv Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
title Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
spellingShingle Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
Carrasco, Hernan J
Trypanosoma cruzi
Trypanosoma cruzi / classificação
Região Amazônica (BR)
title_short Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
title_full Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
title_fullStr Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
title_full_unstemmed Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
title_sort Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi
author Carrasco, Hernan J
author_facet Carrasco, Hernan J
Frame, Iain A
Valente, Sebastião Aldo da Silva
Miles, Michael. A
author_role author
author2 Frame, Iain A
Valente, Sebastião Aldo da Silva
Miles, Michael. A
author2_role author
author
author
dc.contributor.author.fl_str_mv Carrasco, Hernan J
Frame, Iain A
Valente, Sebastião Aldo da Silva
Miles, Michael. A
dc.subject.decsPrimary.pt_BR.fl_str_mv Trypanosoma cruzi
Trypanosoma cruzi / classificação
Região Amazônica (BR)
topic Trypanosoma cruzi
Trypanosoma cruzi / classificação
Região Amazônica (BR)
description Thirty six stocks of Trypanosoma cruzi isolated from sylvatic mammals (32 Didelphis marsupialis and one Philander opossum) and triatomine bugs (Rhodnius robustus and one unidentified bug) in the Amazonian forest of Carajas, Brazil were characterized by isoenzyme and random amplified polymorphic DNA (RAPD) analysis as belonging to principal zymodeme 1 (Zl). Two different homozygous phenotypes and the corresponding heterozygous phenotype were found for phosphoglucomutase with an observed frequency almost identical with that predicted by the theoretical Hardy- Weinberg distribution. Parental and hybrid profiles were algo suggested by RAPD analysis, which allowed exclusion of mixed parental strains from the hybrids: isoenzyme and RAPD profiles of biological clones were algo indistinguishable from those of uncloned stocks. Trypanosoma cruzi stocks from widely separated geographic origins in Central and South America gave similar RAPD profiles that allowed them to be recognized as being Zl. These results indicate that genetic exchange could contribute to the generation of genetic diversity during the sylvatic cycle of T. cruzi, and this may have epidemiologic and taxonomic implications.
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2016-01-26T11:36:39Z
dc.date.available.fl_str_mv 2016-01-26T11:36:39Z
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CARRASCO, Hernan J. et al. Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi. American Journal of Tropical Medicine and Hygiene, v. 54, n. 4, p. 418-424, 1996.
dc.identifier.uri.fl_str_mv https://patua.iec.gov.br/handle/iec/671
dc.identifier.issn.-.fl_str_mv 0002-9637
identifier_str_mv CARRASCO, Hernan J. et al. Genetic exchanges as a possible source of genomic diversity in sylvatic populations of Trypanosoma Cruzi. American Journal of Tropical Medicine and Hygiene, v. 54, n. 4, p. 418-424, 1996.
0002-9637
url https://patua.iec.gov.br/handle/iec/671
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dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
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