Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts

Detalhes bibliográficos
Autor(a) principal: Mattos, Ana Carolina Alves de
Data de Publicação: 2006
Outros Autores: Kusel, John Robert, Pimenta, Paulo Filemon Paolucci, Coelho, Paulo Marcos Zach
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6570
Resumo: Fiocruz-CNPq
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spelling Mattos, Ana Carolina Alves deKusel, John RobertPimenta, Paulo Filemon PaolucciCoelho, Paulo Marcos Zach2013-06-06T14:16:12Z2013-06-06T14:16:12Z2006MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.0074-0276https://www.arca.fiocruz.br/handle/icict/657010.1590/S0074-02762006000900044Fiocruz-CNPqFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.University of Glasgow. IBLS Division of Infection and Immunity. Scotland, UK.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil / Santa Casa de Misericórdia de Belo Horizonte. Belo Horizonte, MG, Brasil.Praziquantel (PZQ) is effective against all the evolutive phases of Schistosoma mansoni. Infected Biomphalaria glabrata snails have their cercarial shedding interrupted when exposed to PZQ. Using primary in vitro transformed sporocysts, labeled with the probe Hoechst 33258 (indicator of membrane integrity), and lectin of Glycine max (specific for carbohydrate of N-acetylgalactosamine membrane), we evaluated the presence of lysosomes at this evolutive phase of S. mansoni, as well as the influence of PZQ on these acidic organelles and on the tegument of the sporocyst. Although the sporocyst remained alive, it was observed that there was a marked contraction of its musculature, and there occurred a change in the parasite’s structure. Also, the acidic vesicles found in the sporo-cysts showed a larger delimited area after contact of the parasites with PZQ. Damages to the tegument was also observed, as show a well-marked labeling either with Hoechst 33258 or with lectin of Glycine max after contact of sporocysts with the drug. These results could partially explain the interruption/reduction mechanism of cercarial shedding in snails exposed to PZQ.engActivity of praziquantel on in vitro transformed Schistosoma mansoni sporocystsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePraziquantelFluorescenceHoechst 33258LysoTracker RedLectin of Glycine maxSporocystsSchistosoma mansoniinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL74.pdf74.pdfapplication/pdf11025228https://www.arca.fiocruz.br/bitstream/icict/6570/1/74.pdf5f9227683c9d83039e74c5484ebe4559MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6570/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT74.pdf.txt74.pdf.txtExtracted texttext/plain20864https://www.arca.fiocruz.br/bitstream/icict/6570/5/74.pdf.txtef8b73578373c2d8ec303efef26dd12eMD55THUMBNAIL74.pdf.jpg74.pdf.jpgGenerated Thumbnailimage/jpeg2179https://www.arca.fiocruz.br/bitstream/icict/6570/4/74.pdf.jpg3b9958dd0b1187ab107bbe97d42ba433MD54icict/65702018-05-11 15:15:26.755oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-05-11T18:15:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
spellingShingle Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
Mattos, Ana Carolina Alves de
Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
title_short Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_full Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_fullStr Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_full_unstemmed Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
title_sort Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts
author Mattos, Ana Carolina Alves de
author_facet Mattos, Ana Carolina Alves de
Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
author_role author
author2 Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
author2_role author
author
author
dc.contributor.author.fl_str_mv Mattos, Ana Carolina Alves de
Kusel, John Robert
Pimenta, Paulo Filemon Paolucci
Coelho, Paulo Marcos Zach
dc.subject.en.pt_BR.fl_str_mv Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
topic Praziquantel
Fluorescence
Hoechst 33258
LysoTracker Red
Lectin of Glycine max
Sporocysts
Schistosoma mansoni
description Fiocruz-CNPq
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-06-06T14:16:12Z
dc.date.available.fl_str_mv 2013-06-06T14:16:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6570
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000900044
identifier_str_mv MATTOS, Ana Carolina Alves de et al. Activity of praziquantel on in vitro transformed Schistosoma mansoni sporocysts. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, suppl.1, p. 283-287, 2006.
0074-0276
10.1590/S0074-02762006000900044
url https://www.arca.fiocruz.br/handle/icict/6570
dc.language.iso.fl_str_mv eng
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