Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil

Detalhes bibliográficos
Autor(a) principal: Cunha, Joana Paixão Monteiro
Data de Publicação: 2011
Outros Autores: Araujo, Adriano Fernando da Silva, Santos, Edson, Castro Filho, Bernardo Galvão, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/1760
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Cunha, Joana Paixão MonteiroAraujo, Adriano Fernando da SilvaSantos, EdsonCastro Filho, Bernardo GalvãoAlcantara, Luiz Carlos Júnior2011-03-26T23:00:19Z2011-03-26T23:00:19Z2011MONTEIRO-CUNHA, J. P. et al. Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil. AIDS Research and Human Retroviruses, v. 27, n.6, p. 623-631, 20110889-222921087197https://www.arca.fiocruz.br/handle/icict/1760engDRAMHIV-1Sequenciamento geneticoResisencia à drogasResistência a MedicamentosHIV-1Análise Mutacional de DNAReação em Cadeia da PolimeraseLack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilNational Cancer Institute. National Institutes of Health. Vaccine Branch. Bethesda, Maryland, USAThe genetic variability and the prevalence of drug resistance-associated mutations (DRAM) of HIV-1 isolates from 50 women and 8 children from Feira de Santana, Bahia, Brazil were investigated. DNA samples were obtained and pol sequences were generated by PCR and direct sequencing. Phylogenetic analysis showed that 39 (67.2%) samples were subtype B, four (6.9%) F, one (1.7%) C, and 14 (24.1%) BF recombinants. Four different BF recombination patterns were detected. Twelve (20.7%) samples shared the same breakpoint within the reverse transcriptase (RT) sequence. Fifty-five (94.8%) isolates showed several resistance-associated mutations in the RT and the protease (PR) genes. Ten (17.2%) isolates presented mutations associated with a high level of resistance: nine (15.5%) to nucleoside RT inhibitors (NRTI), four (6.9%) to nonnucleoside RT inhibitors (NNRTI), and three (5.2%) to PR inhibitors (PIs). Subtype B-infected patients had, on average, 0.5 high-level DRAM per sequence while no mutations were observed in BF recombinants, although the two groups were under ARV for a similar period of time. Our data indicate the predominance of the subtype B, followed by BF recombinants in this population, and the dissemination of a recombinant strain in Bahia, which could be related to adaptive advantages of these variants over the predominant subtype B.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdfLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdfapplication/pdf250008https://www.arca.fiocruz.br/bitstream/icict/1760/1/Lack%20of%20High-Level%20Resistance%20Mutations%20in%20HIV%20Type%201%20%20BF%20Recombinant%20Strains%20Circulating%20in%20Northeast%20Brazil.pdfea95fcce7f255b314416de59f62bab29MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://www.arca.fiocruz.br/bitstream/icict/1760/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdf.txtLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdf.txtExtracted texttext/plain45812https://www.arca.fiocruz.br/bitstream/icict/1760/5/Lack%20of%20High-Level%20Resistance%20Mutations%20in%20HIV%20Type%201%20%20BF%20Recombinant%20Strains%20Circulating%20in%20Northeast%20Brazil.pdf.txt4b9c7a5c238c7bed40a8c49770ef0de7MD55THUMBNAILLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdf.jpgLack of High-Level Resistance Mutations in HIV Type 1 BF Recombinant Strains Circulating in Northeast Brazil.pdf.jpgGenerated Thumbnailimage/jpeg2170https://www.arca.fiocruz.br/bitstream/icict/1760/4/Lack%20of%20High-Level%20Resistance%20Mutations%20in%20HIV%20Type%201%20%20BF%20Recombinant%20Strains%20Circulating%20in%20Northeast%20Brazil.pdf.jpgc0358335a64d9e94722a0944c91cbcacMD54icict/17602023-03-15 14:33:08.443oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:08Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
title Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
spellingShingle Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
Cunha, Joana Paixão Monteiro
DRAM
HIV-1
Sequenciamento genetico
Resisencia à drogas
Resistência a Medicamentos
HIV-1
Análise Mutacional de DNA
Reação em Cadeia da Polimerase
title_short Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
title_full Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
title_fullStr Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
title_full_unstemmed Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
title_sort Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil
author Cunha, Joana Paixão Monteiro
author_facet Cunha, Joana Paixão Monteiro
Araujo, Adriano Fernando da Silva
Santos, Edson
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author_role author
author2 Araujo, Adriano Fernando da Silva
Santos, Edson
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Joana Paixão Monteiro
Araujo, Adriano Fernando da Silva
Santos, Edson
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
dc.subject.other.pt_BR.fl_str_mv DRAM
HIV-1
Sequenciamento genetico
Resisencia à drogas
topic DRAM
HIV-1
Sequenciamento genetico
Resisencia à drogas
Resistência a Medicamentos
HIV-1
Análise Mutacional de DNA
Reação em Cadeia da Polimerase
dc.subject.decs.pt_BR.fl_str_mv Resistência a Medicamentos
HIV-1
Análise Mutacional de DNA
Reação em Cadeia da Polimerase
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2011
dc.date.accessioned.fl_str_mv 2011-03-26T23:00:19Z
dc.date.available.fl_str_mv 2011-03-26T23:00:19Z
dc.date.issued.fl_str_mv 2011
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MONTEIRO-CUNHA, J. P. et al. Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil. AIDS Research and Human Retroviruses, v. 27, n.6, p. 623-631, 2011
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/1760
dc.identifier.issn.pt_BR.fl_str_mv 0889-2229
dc.identifier.other.none.fl_str_mv 21087197
identifier_str_mv MONTEIRO-CUNHA, J. P. et al. Lack of high-level resistance mutations in HIV Type 1 BF recombinant strains circulating in northeast Brazil. AIDS Research and Human Retroviruses, v. 27, n.6, p. 623-631, 2011
0889-2229
21087197
url https://www.arca.fiocruz.br/handle/icict/1760
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