Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2808 |
Resumo: | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), the Brazilian Ministry of Health STD/AIDS Program, CNPq, and FIOCRUZ |
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Monteiro, Joana PaixãoFerraro, Geraldo ArgoloOliveira, Tulio deGoldani, Luciano ZKashima, Simone GAlcantara, Luiz Carlos JuniorMorgado, Mariza GonçalvesBou-Habib, Dumith ChequerCastro Filho, Bernardo Galvão2011-08-21T01:32:46Z2011-08-21T01:32:46Z2007MONTEIRO, J. P. Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil. AIDS Research and Human Retroviruses, v. 23, n. 1, p. 135-143, 2007.0889-2229https://www.arca.fiocruz.br/handle/icict/280810.1089/aid.2006.0064Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), the Brazilian Ministry of Health STD/AIDS Program, CNPq, and FIOCRUZFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Oxford. Department of Zoology. Oxford, UKUniversidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Porto Alegre, RS, BrasilUniversidade de São Paulo. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA. BrasilThe molecular and biological properties of HIV-1 subtype C strains from South Brazil were investigated. We sequenced gag and env fragments of viruses from 22 HIV-1-infected individuals from Porto Alegre City, which has the highest frequency of subtype C in the country. The sequences were then compared with other subtype B, C, and F strains isolated in Brazil and other countries using phylogenetic methods. Amino acid signatures were identified and correlated with phenotypic characteristics. We identified six strains with subtype C (27.3%), eight subtype B (36.4%), one subtype F (4.5%), six C/B recombinants (27.3%), and one B/F recombinant (4.5%). The Brazilian subtype C sequences formed a unique phylogenetic group and presented 6 and 18 specific amino acid signatures in gag and env, respectively. Three distinct patterns of C/B recombinants presented characteristic Brazilian amino acid substitutions. Subtype C viruses were predominantly R5 and non-syncytium-inducing, while C/B recombinants were R5/X4 and syncytium-inducing viruses. These findings suggest that subtype C viruses circulating in Brazil are the result of a unique introduction into the country. Recombination events between subtypes B and C have been occurring frequently for more than 10 years in South Brazil. Biological characterization confirms the hypothesis that subtype C is distinct from the others in the evolution of coreceptor utilization.engGenetic and biologic characterization of HIV type 1 subtype C isolates from south Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInfecções por HIVHIV-1Recombinação GenéticaDNA ViralReação em Cadeia da PolimeraseAnálise de Sequência de DNAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALP - Genetic and biologic characterization of HIV.pdfP - Genetic and biologic characterization of HIV.pdfapplication/pdf2186482https://www.arca.fiocruz.br/bitstream/icict/2808/1/P%20-%20Genetic%20and%20biologic%20characterization%20of%20HIV.pdf1cc3f7bc914316b3ca52737635fb86c8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2808/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTP - Genetic and biologic characterization of HIV.pdf.txtP - Genetic and biologic characterization of HIV.pdf.txtExtracted texttext/plain36467https://www.arca.fiocruz.br/bitstream/icict/2808/5/P%20-%20Genetic%20and%20biologic%20characterization%20of%20HIV.pdf.txt4d4353875fe8e59436935bc96b323023MD55THUMBNAILP - Genetic and biologic characterization of HIV.pdf.jpgP - Genetic and biologic characterization of HIV.pdf.jpgGenerated Thumbnailimage/jpeg2256https://www.arca.fiocruz.br/bitstream/icict/2808/4/P%20-%20Genetic%20and%20biologic%20characterization%20of%20HIV.pdf.jpga5631a0395d79d007b087c12d11808bcMD54icict/28082023-09-04 10:37:10.357oai:www.arca.fiocruz.br:icict/2808Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKQnkgc2lnbmluZyBhbmQgc3VibWl0dGluZyB0aGlzIGxpY2Vuc2UsIHlvdSAodGhlIGF1dGhvcihzKSBvciBjb3B5cmlnaHQKb3duZXIpIGdyYW50cyB0byBEU3BhY2UgVW5pdmVyc2l0eSAoRFNVKSB0aGUgbm9uLWV4Y2x1c2l2ZSByaWdodCB0byByZXByb2R1Y2UsCnRyYW5zbGF0ZSAoYXMgZGVmaW5lZCBiZWxvdyksIGFuZC9vciBkaXN0cmlidXRlIHlvdXIgc3VibWlzc2lvbiAoaW5jbHVkaW5nCnRoZSBhYnN0cmFjdCkgd29ybGR3aWRlIGluIHByaW50IGFuZCBlbGVjdHJvbmljIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bSwKaW5jbHVkaW5nIGJ1dCBub3QgbGltaXRlZCB0byBhdWRpbyBvciB2aWRlby4KCllvdSBhZ3JlZSB0aGF0IERTVSBtYXksIHdpdGhvdXQgY2hhbmdpbmcgdGhlIGNvbnRlbnQsIHRyYW5zbGF0ZSB0aGUKc3VibWlzc2lvbiB0byBhbnkgbWVkaXVtIG9yIGZvcm1hdCBmb3IgdGhlIHB1cnBvc2Ugb2YgcHJlc2VydmF0aW9uLgoKWW91IGFsc28gYWdyZWUgdGhhdCBEU1UgbWF5IGtlZXAgbW9yZSB0aGFuIG9uZSBjb3B5IG9mIHRoaXMgc3VibWlzc2lvbiBmb3IKcHVycG9zZXMgb2Ygc2VjdXJpdHksIGJhY2stdXAgYW5kIHByZXNlcnZhdGlvbi4KCllvdSByZXByZXNlbnQgdGhhdCB0aGUgc3VibWlzc2lvbiBpcyB5b3VyIG9yaWdpbmFsIHdvcmssIGFuZCB0aGF0IHlvdSBoYXZlCnRoZSByaWdodCB0byBncmFudCB0aGUgcmlnaHRzIGNvbnRhaW5lZCBpbiB0aGlzIGxpY2Vuc2UuIFlvdSBhbHNvIHJlcHJlc2VudAp0aGF0IHlvdXIgc3VibWlzc2lvbiBkb2VzIG5vdCwgdG8gdGhlIGJlc3Qgb2YgeW91ciBrbm93bGVkZ2UsIGluZnJpbmdlIHVwb24KYW55b25lJ3MgY29weXJpZ2h0LgoKSWYgdGhlIHN1Ym1pc3Npb24gY29udGFpbnMgbWF0ZXJpYWwgZm9yIHdoaWNoIHlvdSBkbyBub3QgaG9sZCBjb3B5cmlnaHQsCnlvdSByZXByZXNlbnQgdGhhdCB5b3UgaGF2ZSBvYnRhaW5lZCB0aGUgdW5yZXN0cmljdGVkIHBlcm1pc3Npb24gb2YgdGhlCmNvcHlyaWdodCBvd25lciB0byBncmFudCBEU1UgdGhlIHJpZ2h0cyByZXF1aXJlZCBieSB0aGlzIGxpY2Vuc2UsIGFuZCB0aGF0CnN1Y2ggdGhpcmQtcGFydHkgb3duZWQgbWF0ZXJpYWwgaXMgY2xlYXJseSBpZGVudGlmaWVkIGFuZCBhY2tub3dsZWRnZWQKd2l0aGluIHRoZSB0ZXh0IG9yIGNvbnRlbnQgb2YgdGhlIHN1Ym1pc3Npb24uCgpJRiBUSEUgU1VCTUlTU0lPTiBJUyBCQVNFRCBVUE9OIFdPUksgVEhBVCBIQVMgQkVFTiBTUE9OU09SRUQgT1IgU1VQUE9SVEVECkJZIEFOIEFHRU5DWSBPUiBPUkdBTklaQVRJT04gT1RIRVIgVEhBTiBEU1UsIFlPVSBSRVBSRVNFTlQgVEhBVCBZT1UgSEFWRQpGVUxGSUxMRUQgQU5ZIFJJR0hUIE9GIFJFVklFVyBPUiBPVEhFUiBPQkxJR0FUSU9OUyBSRVFVSVJFRCBCWSBTVUNICkNPTlRSQUNUIE9SIEFHUkVFTUVOVC4KCkRTVSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgeW91ciBuYW1lKHMpIGFzIHRoZSBhdXRob3Iocykgb3Igb3duZXIocykgb2YgdGhlCnN1Ym1pc3Npb24sIGFuZCB3aWxsIG5vdCBtYWtlIGFueSBhbHRlcmF0aW9uLCBvdGhlciB0aGFuIGFzIGFsbG93ZWQgYnkgdGhpcwpsaWNlbnNlLCB0byB5b3VyIHN1Ym1pc3Npb24uCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T13:37:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| title |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| spellingShingle |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil Monteiro, Joana Paixão Infecções por HIV HIV-1 Recombinação Genética DNA Viral Reação em Cadeia da Polimerase Análise de Sequência de DNA |
| title_short |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| title_full |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| title_fullStr |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| title_full_unstemmed |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| title_sort |
Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil |
| author |
Monteiro, Joana Paixão |
| author_facet |
Monteiro, Joana Paixão Ferraro, Geraldo Argolo Oliveira, Tulio de Goldani, Luciano Z Kashima, Simone G Alcantara, Luiz Carlos Junior Morgado, Mariza Gonçalves Bou-Habib, Dumith Chequer Castro Filho, Bernardo Galvão |
| author_role |
author |
| author2 |
Ferraro, Geraldo Argolo Oliveira, Tulio de Goldani, Luciano Z Kashima, Simone G Alcantara, Luiz Carlos Junior Morgado, Mariza Gonçalves Bou-Habib, Dumith Chequer Castro Filho, Bernardo Galvão |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Monteiro, Joana Paixão Ferraro, Geraldo Argolo Oliveira, Tulio de Goldani, Luciano Z Kashima, Simone G Alcantara, Luiz Carlos Junior Morgado, Mariza Gonçalves Bou-Habib, Dumith Chequer Castro Filho, Bernardo Galvão |
| dc.subject.decs.pt_BR.fl_str_mv |
Infecções por HIV HIV-1 Recombinação Genética DNA Viral Reação em Cadeia da Polimerase Análise de Sequência de DNA |
| topic |
Infecções por HIV HIV-1 Recombinação Genética DNA Viral Reação em Cadeia da Polimerase Análise de Sequência de DNA |
| description |
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), the Brazilian Ministry of Health STD/AIDS Program, CNPq, and FIOCRUZ |
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2007 |
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2007 |
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2011-08-21T01:32:46Z |
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2011-08-21T01:32:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MONTEIRO, J. P. Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil. AIDS Research and Human Retroviruses, v. 23, n. 1, p. 135-143, 2007. |
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0889-2229 |
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10.1089/aid.2006.0064 |
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MONTEIRO, J. P. Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil. AIDS Research and Human Retroviruses, v. 23, n. 1, p. 135-143, 2007. 0889-2229 10.1089/aid.2006.0064 |
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