Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24983 |
Resumo: | CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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Cardoso, Thiago MarconiMachado, ÁlvaroCosta, Diego LuizCarvalho, Lucas Pedreira deQueiroz, AdrianoMachado, PauloScott, PhillipCarvalho Filho, Edgar MarcelinoBacellar, Olívia2018-02-27T16:57:16Z2018-02-27T16:57:16Z2015CARDOSO, T. M. et al. Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection. Infection and Immunity, v. 83, n. 3, p. 898-906, 2015.0019-9567https://www.arca.fiocruz.br/handle/icict/2498310.1128/IAI.02404-14CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institutes of Health (NIH) grant AI30639-20.Universidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, BrazilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversity of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, Pennsylvania, USAUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais. Salvador, BA, BrasilCutaneous leishmaniasis (CL) caused by Leishmania braziliensis is characterized by a strong Th1 response that leads to skin lesion development. In areas where L. braziliensis transmission is endemic, up to 15% of healthy subjects have tested positive for delayed-type hypersensitivity to soluble leishmania antigen (SLA) and are considered to have subclinical (SC) infection. SC subjects produce less gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) than do CL patients, but they are able to control the infection. The aim of this study was to characterized the role of CD8(+) T cells in SC infection and in CL. Peripheral blood mononuclear cells (PBMC) were stimulated with SLA to determine the frequencies of CD4(+) IFN-γ(+) and CD8(+) IFN-γ(+) T cells. Monocytes from PBMC were infected with L. braziliensis and cocultured with CD8(+) T cells, and the frequencies of infected monocytes and levels of cytotoxicity markers, target cell apoptosis, and granzyme B were determined. The frequency of CD8(+) IFN-γ(+) cells after SLA stimulation was higher for SC individuals than for CL patients. The frequency of infected monocytes in SC cells was lower than that in CL cells. CL CD8(+) T cells induced more apoptosis of infected monocytes than did SC CD8(+) T cells. Granzyme B production in CD8(+) T cells was higher in CL than in SC cells. While the use of a granzyme B inhibitor decreased the number of apoptotic cells in the CL group, the use of z-VAD-FMK had no effect on the frequency of these cells. These results suggest that CL CD8(+) T cells are more cytotoxic and may be involved in pathology.engAmerican Society for MicrobiologyLeishmania braziliensisLeishmaniose CutâneaMonócitosInterferon gamaFator de necrose tumoral alfaLeishmania braziliensisLeishmaniasis, CutaneousMonocytesInterferon-gammaTumor Necrosis Factor-alphaProtective and pathological functions of CD8+ T cells in Leishmania braziliensis infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24983/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCardoso TM Protective and pathological functions....pdfCardoso TM Protective and pathological 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| dc.title.pt_BR.fl_str_mv |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| title |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| spellingShingle |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection Cardoso, Thiago Marconi Leishmania braziliensis Leishmaniose Cutânea Monócitos Interferon gama Fator de necrose tumoral alfa Leishmania braziliensis Leishmaniasis, Cutaneous Monocytes Interferon-gamma Tumor Necrosis Factor-alpha |
| title_short |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| title_full |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| title_fullStr |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| title_full_unstemmed |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| title_sort |
Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection |
| author |
Cardoso, Thiago Marconi |
| author_facet |
Cardoso, Thiago Marconi Machado, Álvaro Costa, Diego Luiz Carvalho, Lucas Pedreira de Queiroz, Adriano Machado, Paulo Scott, Phillip Carvalho Filho, Edgar Marcelino Bacellar, Olívia |
| author_role |
author |
| author2 |
Machado, Álvaro Costa, Diego Luiz Carvalho, Lucas Pedreira de Queiroz, Adriano Machado, Paulo Scott, Phillip Carvalho Filho, Edgar Marcelino Bacellar, Olívia |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cardoso, Thiago Marconi Machado, Álvaro Costa, Diego Luiz Carvalho, Lucas Pedreira de Queiroz, Adriano Machado, Paulo Scott, Phillip Carvalho Filho, Edgar Marcelino Bacellar, Olívia |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania braziliensis Leishmaniose Cutânea Monócitos Interferon gama Fator de necrose tumoral alfa |
| topic |
Leishmania braziliensis Leishmaniose Cutânea Monócitos Interferon gama Fator de necrose tumoral alfa Leishmania braziliensis Leishmaniasis, Cutaneous Monocytes Interferon-gamma Tumor Necrosis Factor-alpha |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania braziliensis Leishmaniasis, Cutaneous Monocytes Interferon-gamma Tumor Necrosis Factor-alpha |
| description |
CARVALHO FILHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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2015 |
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2015 |
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2018-02-27T16:57:16Z |
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2018-02-27T16:57:16Z |
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info:eu-repo/semantics/article |
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CARDOSO, T. M. et al. Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection. Infection and Immunity, v. 83, n. 3, p. 898-906, 2015. |
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https://www.arca.fiocruz.br/handle/icict/24983 |
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0019-9567 |
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10.1128/IAI.02404-14 |
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CARDOSO, T. M. et al. Protective and pathological functions of CD8+ T cells in Leishmania braziliensis infection. Infection and Immunity, v. 83, n. 3, p. 898-906, 2015. 0019-9567 10.1128/IAI.02404-14 |
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American Society for Microbiology |
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