Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing

Detalhes bibliográficos
Autor(a) principal: Romão, Célia Maria Carvalho Pereira Araujo
Data de Publicação: 2005
Outros Autores: Faria, Yaisa Naziozeno de, Pereira, Luciana Roberto, Asensi, Marise Dutra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33479
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
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spelling Romão, Célia Maria Carvalho Pereira AraujoFaria, Yaisa Naziozeno dePereira, Luciana RobertoAsensi, Marise Dutra2019-06-13T11:08:26Z2019-06-13T11:08:26Z2005ROMÃO, Célia Maria Carvalho Pereira Araujo et al. Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 5, p. 541-548, Aug. 2005.0074-0276https://www.arca.fiocruz.br/handle/icict/3347910.1590/s0074-027620050005000151678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzPseudomonas aeruginosaEletroforese em gel de campo pulsadoDesinfetantesPseudomonas aeruginosaDisinfectantsPulsed field gel electrophoresisSusceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.The aim of this study was to evaluate the susceptibility of 35 resistant Pseudomonas aeruginosa clinical isolates to a quaternary ammonium hospital disinfectant. The methodology was the AOAC Use-Dilution Test, with disinfectant at its use-concentration. In addition, the chromosomal DNA profile of the isolates were determined by macrorestriction pulsed field gel electrophoresis (PFGE) method aiming to verify the relatedness among them and the behavior of isolates from the same group regarding the susceptibility to the disinfectant. Seventy one percent of the isolates were multiresistant to antibiotics and 43% showed a reduced susceptibility to the disinfectant. The PFGE methodology detected 18 major clonal groups. We found isolates with reduced susceptibility to the disinfectant and we think that these are worrying data that should be further investigated including different organisms and chemical agents in order to demonstrate that microorganisms can be destroyed by biocide as necessary. We also found strains of the same clonal groups showing different susceptibility to the disinfectant. This is an interesting observation considering that only few works are available about this subject. PFGE profile seems not to be a reliable marker for resistance to disinfectants.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33479/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCeliaRomao_MariseAsensi_etal_IOC_2005.pdfCeliaRomao_MariseAsensi_etal_IOC_2005.pdfapplication/pdf54231https://www.arca.fiocruz.br/bitstream/icict/33479/2/CeliaRomao_MariseAsensi_etal_IOC_2005.pdfe4749e7939e254dd1cfff9d1217cb48cMD52TEXTCeliaRomao_MariseAsensi_etal_IOC_2005.pdf.txtCeliaRomao_MariseAsensi_etal_IOC_2005.pdf.txtExtracted texttext/plain37973https://www.arca.fiocruz.br/bitstream/icict/33479/3/CeliaRomao_MariseAsensi_etal_IOC_2005.pdf.txta28299fcb09602d7634b1a71e23d128bMD53icict/334792019-06-15 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dc.title.pt_BR.fl_str_mv Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
title Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
spellingShingle Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
Romão, Célia Maria Carvalho Pereira Araujo
Pseudomonas aeruginosa
Eletroforese em gel de campo pulsado
Desinfetantes
Pseudomonas aeruginosa
Disinfectants
Pulsed field gel electrophoresis
title_short Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
title_full Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
title_fullStr Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
title_full_unstemmed Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
title_sort Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing
author Romão, Célia Maria Carvalho Pereira Araujo
author_facet Romão, Célia Maria Carvalho Pereira Araujo
Faria, Yaisa Naziozeno de
Pereira, Luciana Roberto
Asensi, Marise Dutra
author_role author
author2 Faria, Yaisa Naziozeno de
Pereira, Luciana Roberto
Asensi, Marise Dutra
author2_role author
author
author
dc.contributor.author.fl_str_mv Romão, Célia Maria Carvalho Pereira Araujo
Faria, Yaisa Naziozeno de
Pereira, Luciana Roberto
Asensi, Marise Dutra
dc.subject.other.pt_BR.fl_str_mv Pseudomonas aeruginosa
Eletroforese em gel de campo pulsado
Desinfetantes
topic Pseudomonas aeruginosa
Eletroforese em gel de campo pulsado
Desinfetantes
Pseudomonas aeruginosa
Disinfectants
Pulsed field gel electrophoresis
dc.subject.en.pt_BR.fl_str_mv Pseudomonas aeruginosa
Disinfectants
Pulsed field gel electrophoresis
description Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-06-13T11:08:26Z
dc.date.available.fl_str_mv 2019-06-13T11:08:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ROMÃO, Célia Maria Carvalho Pereira Araujo et al. Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 5, p. 541-548, Aug. 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33479
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/s0074-02762005000500015
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ROMÃO, Célia Maria Carvalho Pereira Araujo et al. Susceptibility of clinical isolates of multiresistant Pseudomonas aeruginosa to a hospital disinfectant and molecular typing. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 5, p. 541-548, Aug. 2005.
0074-0276
10.1590/s0074-02762005000500015
1678-8060
url https://www.arca.fiocruz.br/handle/icict/33479
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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