Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMS |
Texto Completo: | https://repositorio.ufms.br/handle/123456789/1252 http://dx.doi.org/10.1590/S1413-86702011000300002. |
Resumo: | Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals. |
id |
UFMS_153abec009e74da31b8808d8e9a36387 |
---|---|
oai_identifier_str |
oai:repositorio.ufms.br:123456789/1252 |
network_acronym_str |
UFMS |
network_name_str |
Repositório Institucional da UFMS |
repository_id_str |
2124 |
spelling |
2012-04-17T12:33:05Z2021-09-30T19:56:44Z2011-05RODRIGUES, Ana Claudia Souza et al . Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, Salvador, v. 15, n. 3, June 2011 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002&lng=en&nrm=iso>. access on 17 Apr. 2012.https://repositorio.ufms.br/handle/123456789/1252http://dx.doi.org/10.1590/S1413-86702011000300002.Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.engBrazilian Journal of Infectious DiseasesPseudomonas AeruginosaResistência a MedicamentosBeta-LactamasesEletroforeseEletroforese em Gel de Campo PulsadoDrug ResistanceElectrophoresisElectrophoresis, Gel, Pulsed-FieldMetallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRodrigues, Ana Claudia SouzaChang, Marilene RodriguesNóbrega, Gabriela DornRodrigues, Mayara SouzaCarvalho, Nadia Cristina PereiraGomes, Beatriz GarciaAlmeida, Dênio Lopes deCarvalho, Karyne RangelAsensi, Marise Dutrainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMSinstname:Universidade Federal de Mato Grosso do Sul (UFMS)instacron:UFMSTHUMBNAILMetallo-β-lactamase and genetic diversity.pdf.jpgMetallo-β-lactamase and genetic diversity.pdf.jpgGenerated Thumbnailimage/jpeg1727https://repositorio.ufms.br/bitstream/123456789/1252/4/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf.jpge4e4dfa681b8f64e4ed5681590f0f1f8MD54ORIGINALMetallo-β-lactamase and genetic diversity.pdfMetallo-β-lactamase and genetic diversity.pdfapplication/pdf843541https://repositorio.ufms.br/bitstream/123456789/1252/1/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf8594775c259bbf27a611e72274e20ea9MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufms.br/bitstream/123456789/1252/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTMetallo-β-lactamase and genetic diversity.pdf.txtMetallo-β-lactamase and genetic diversity.pdf.txtExtracted texttext/plain24655https://repositorio.ufms.br/bitstream/123456789/1252/3/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf.txt44d5f9e63a588753422539d63123ce7aMD53123456789/12522021-09-30 15:56:45.012oai:repositorio.ufms.br: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Repositório InstitucionalPUBhttps://repositorio.ufms.br/oai/requestri.prograd@ufms.bropendoar:21242021-09-30T19:56:45Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS)false |
dc.title.pt_BR.fl_str_mv |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
title |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
spellingShingle |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil Rodrigues, Ana Claudia Souza Pseudomonas Aeruginosa Resistência a Medicamentos Beta-Lactamases Eletroforese Eletroforese em Gel de Campo Pulsado Drug Resistance Electrophoresis Electrophoresis, Gel, Pulsed-Field |
title_short |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
title_full |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
title_fullStr |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
title_full_unstemmed |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
title_sort |
Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil |
author |
Rodrigues, Ana Claudia Souza |
author_facet |
Rodrigues, Ana Claudia Souza Chang, Marilene Rodrigues Nóbrega, Gabriela Dorn Rodrigues, Mayara Souza Carvalho, Nadia Cristina Pereira Gomes, Beatriz Garcia Almeida, Dênio Lopes de Carvalho, Karyne Rangel Asensi, Marise Dutra |
author_role |
author |
author2 |
Chang, Marilene Rodrigues Nóbrega, Gabriela Dorn Rodrigues, Mayara Souza Carvalho, Nadia Cristina Pereira Gomes, Beatriz Garcia Almeida, Dênio Lopes de Carvalho, Karyne Rangel Asensi, Marise Dutra |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Ana Claudia Souza Chang, Marilene Rodrigues Nóbrega, Gabriela Dorn Rodrigues, Mayara Souza Carvalho, Nadia Cristina Pereira Gomes, Beatriz Garcia Almeida, Dênio Lopes de Carvalho, Karyne Rangel Asensi, Marise Dutra |
dc.subject.por.fl_str_mv |
Pseudomonas Aeruginosa Resistência a Medicamentos Beta-Lactamases Eletroforese Eletroforese em Gel de Campo Pulsado Drug Resistance Electrophoresis Electrophoresis, Gel, Pulsed-Field |
topic |
Pseudomonas Aeruginosa Resistência a Medicamentos Beta-Lactamases Eletroforese Eletroforese em Gel de Campo Pulsado Drug Resistance Electrophoresis Electrophoresis, Gel, Pulsed-Field |
description |
Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-05 |
dc.date.accessioned.fl_str_mv |
2012-04-17T12:33:05Z |
dc.date.available.fl_str_mv |
2021-09-30T19:56:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Ana Claudia Souza et al . Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, Salvador, v. 15, n. 3, June 2011 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002&lng=en&nrm=iso>. access on 17 Apr. 2012. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufms.br/handle/123456789/1252 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1590/S1413-86702011000300002. |
identifier_str_mv |
RODRIGUES, Ana Claudia Souza et al . Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, Salvador, v. 15, n. 3, June 2011 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002&lng=en&nrm=iso>. access on 17 Apr. 2012. |
url |
https://repositorio.ufms.br/handle/123456789/1252 http://dx.doi.org/10.1590/S1413-86702011000300002. |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Brazilian Journal of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Journal of Infectious Diseases |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFMS instname:Universidade Federal de Mato Grosso do Sul (UFMS) instacron:UFMS |
instname_str |
Universidade Federal de Mato Grosso do Sul (UFMS) |
instacron_str |
UFMS |
institution |
UFMS |
reponame_str |
Repositório Institucional da UFMS |
collection |
Repositório Institucional da UFMS |
bitstream.url.fl_str_mv |
https://repositorio.ufms.br/bitstream/123456789/1252/4/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf.jpg https://repositorio.ufms.br/bitstream/123456789/1252/1/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf https://repositorio.ufms.br/bitstream/123456789/1252/2/license.txt https://repositorio.ufms.br/bitstream/123456789/1252/3/Metallo-%ce%b2-lactamase%20and%20genetic%20diversity.pdf.txt |
bitstream.checksum.fl_str_mv |
e4e4dfa681b8f64e4ed5681590f0f1f8 8594775c259bbf27a611e72274e20ea9 8a4605be74aa9ea9d79846c1fba20a33 44d5f9e63a588753422539d63123ce7a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFMS - Universidade Federal de Mato Grosso do Sul (UFMS) |
repository.mail.fl_str_mv |
ri.prograd@ufms.br |
_version_ |
1801678563933421568 |