Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Gravina, Humberto Doriguêtto
Data de Publicação: 2013
Outros Autores: Antonelli, Lis Ribeiro do Valle, Gazzinelli, Ricardo Tostes, Ropert, Catherine
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30413
Resumo: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.
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spelling Gravina, Humberto DoriguêttoAntonelli, Lis Ribeiro do ValleGazzinelli, Ricardo TostesRopert, Catherine2018-12-05T18:20:21Z2018-12-05T18:20:21Z2013GRAVINA, Humberto Doriguêtto et al. Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi. PLoS One, v. 8, n. 5, e63100, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/3041310.1371/journal.pone.0063100engPublic Library of ScienceTrypanosoma cruziDoença de chagasTrypanosoma cruziChagas diseaseDifferential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil/Division of Infectious Diseases and Immunology. University of Massachusetts Medical School. Worcester, MA, Untied States of America.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilPathogens express ligands for several TLRs that may play a role in the induction or control of the inflammatory response during infection. Concerning Trypanosoma cruzi, the agent of Chagas disease, we have previously characterized glycosylphosphatidylinositol (GPI) anchored mucin-like glycoproteins (tGPI-mucin) and unmethylated CpG DNA sequences as TLR2 and TLR9 agonists, respectively. Here we sought to determine how these TLRs may modulate the inflammatory response in the following cell populations: F4/80+CD11b+ (macrophages), F4/80lowCD11b+ (monocytes) and MHCII+CD11chigh (dendritic cells). For this purpose, TLR2−/− and TLR9−/− mice were infected with Y strain of T. cruzi and different immunological parameters were evaluated. According to our previous data, a crucial role of TLR9 was evidenced in the establishment of Th1 response, whereas TLR2 appeared to act as immunoregulator in the early stage of infection. More precisely, we demonstrated here that TLR2 was mainly used by F4/80+CD11b+ cells for the production of TNF-α. In the absence of TLR2, an increased production of IL-12/IL-23p40 and IFN-γ was noted suggesting that TLR2 negatively controls the Th1 response. In contrast, TLR9 was committed to IL-12/IL-23p40 production by MHCII+CD11chigh cells that constitute the main source of IL-12/IL-23p40 during infection. Importantly, a down-regulation of TLR9 response was observed in F4/80+CD11b+ and F4/80lowCD11b+ populations that correlated with the decreased TLR9 expression level in these cells. Interestingly, these cells recovered their capacity to respond to TLR9 agonist when MHCII+CD11chigh cells were impeded from producing IL-12/IL-23p40, thereby indicating possible cross-talk between these populations. The differential use of TLR2 and TLR9 by the immune cells during the acute phase of the infection explains why TLR9- but not TLR2-deficient mice are susceptible to T. cruzi infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30413/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALDifferential Use of TLR2 and TLR9 .pdfDifferential Use of TLR2 and TLR9 .pdfapplication/pdf5776313https://www.arca.fiocruz.br/bitstream/icict/30413/2/Differential%20Use%20of%20TLR2%20and%20TLR9%20.pdf3206b5a0f2e440a9c95d8318a58fc1f8MD52TEXTDifferential Use of TLR2 and TLR9 .pdf.txtDifferential Use of TLR2 and TLR9 .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
title Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
spellingShingle Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
Gravina, Humberto Doriguêtto
Trypanosoma cruzi
Doença de chagas
Trypanosoma cruzi
Chagas disease
title_short Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
title_full Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
title_fullStr Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
title_full_unstemmed Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
title_sort Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi
author Gravina, Humberto Doriguêtto
author_facet Gravina, Humberto Doriguêtto
Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
Ropert, Catherine
author_role author
author2 Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
Ropert, Catherine
author2_role author
author
author
dc.contributor.author.fl_str_mv Gravina, Humberto Doriguêtto
Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
Ropert, Catherine
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de chagas
topic Trypanosoma cruzi
Doença de chagas
Trypanosoma cruzi
Chagas disease
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chagas disease
description Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-12-05T18:20:21Z
dc.date.available.fl_str_mv 2018-12-05T18:20:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GRAVINA, Humberto Doriguêtto et al. Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi. PLoS One, v. 8, n. 5, e63100, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30413
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0063100
identifier_str_mv GRAVINA, Humberto Doriguêtto et al. Differential Use of TLR2 and TLR9 in the Regulation of Immune Responses during the Infection with Trypanosoma cruzi. PLoS One, v. 8, n. 5, e63100, 2013.
1932-6203
10.1371/journal.pone.0063100
url https://www.arca.fiocruz.br/handle/icict/30413
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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