Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis

Bibliographic Details
Main Author: Peixoto, Fábio de Carvalho
Publication Date: 2020
Other Authors: Nascimento, Maurício, Costa, Rúbia, Silva, Juliana, Renard, Gaby, Guimarães, Luiz Henrique, Penna, Gerson Oliveira, Barral-Netto, Manoel, Carvalho, Lucas P., Machado, Paulo Roberto Lima, Carvalho, Edgar M.
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/42614
Summary: Gerson Oliveira Penna - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento do vinculo com a unidade da Fiocruz Brasília.
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spelling Peixoto, Fábio de CarvalhoNascimento, MaurícioCosta, RúbiaSilva, JulianaRenard, GabyGuimarães, Luiz HenriquePenna, Gerson OliveiraBarral-Netto, ManoelCarvalho, Lucas P.Machado, Paulo Roberto LimaCarvalho, Edgar M.2020-08-10T15:50:08Z2020-08-10T15:50:08Z2020PEIXOTO, Fábio et al. Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis. Journal of Immunology Research, [s.l], v. 2020, n. 2789859, p. 1-9, Aug. 2020.2314-7156https://www.arca.fiocruz.br/handle/icict/4261410.1155/2020/2789859Gerson Oliveira Penna - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento do vinculo com a unidade da Fiocruz Brasília.Ministério da Saúde e pelo Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Quatro G Pesquisa & Desenvolvimento Ltda. Porto Alegre, RS, Brasil.Universidade de Brasília. Faculdade de Medicina. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Cutaneous leishmaniasis (CL) due to L. braziliensis is associated with an exaggerated inflammatory response and tissue damage. Miltefosine is more effective than pentavalent antimony (Sbv ) in the treatment of CL, and here, we evaluate the ability of Sbv , miltefosine, and GM-CSF administered intravenously, orally, or topically, respectively, to modify the immune response. Patients were treated with miltefosine plus GM-CSF, miltefosine plus placebo, or Sbv . Mononuclear cells were stimulated with soluble Leishmania antigen (SLA) on day 0 and day 15 of therapy, and cytokine levels were determined in supernatants by ELISA. The lymphocyte proliferation and oxidative burst were evaluated by flow cytometry, and the degree of infection and Leishmania killing by optical microscopy. Proliferation of CD4+ T cells were enhanced in patients using miltefosine and in CD8+ T cells when GM-CSF was associated. Enhancement in the oxidative burst occurred in the miltefosine plus GM-CSF group on day 15 of therapy. Moreover, the number of L. braziliensis in infected monocytes on day 15 as well as the percentage of infected cells was lower after 48- and 72-hour culture in cells from patients treated with miltefosine plus GM-CSF. In addition to the ability of miltefosine to kill Leishmania, the changes in the immune response caused by miltefosine and GM-CSF may increase the cure rate of CL patients using these drugs.engHindawiAdministration, OralAntiprotozoal AgentsClinical Trials as TopicLeishmaniasisCutaneous leishmaniasisMiltefosineLeishmanioseLeishmaniose CutâneaEnsaios Clínicos como AssuntoResultado do TratamentoEvaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
title Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
spellingShingle Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
Peixoto, Fábio de Carvalho
Administration, Oral
Antiprotozoal Agents
Clinical Trials as Topic
Leishmaniasis
Cutaneous leishmaniasis
Miltefosine
Leishmaniose
Leishmaniose Cutânea
Ensaios Clínicos como Assunto
Resultado do Tratamento
title_short Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
title_full Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
title_fullStr Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
title_full_unstemmed Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
title_sort Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis
author Peixoto, Fábio de Carvalho
author_facet Peixoto, Fábio de Carvalho
Nascimento, Maurício
Costa, Rúbia
Silva, Juliana
Renard, Gaby
Guimarães, Luiz Henrique
Penna, Gerson Oliveira
Barral-Netto, Manoel
Carvalho, Lucas P.
Machado, Paulo Roberto Lima
Carvalho, Edgar M.
author_role author
author2 Nascimento, Maurício
Costa, Rúbia
Silva, Juliana
Renard, Gaby
Guimarães, Luiz Henrique
Penna, Gerson Oliveira
Barral-Netto, Manoel
Carvalho, Lucas P.
Machado, Paulo Roberto Lima
Carvalho, Edgar M.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Peixoto, Fábio de Carvalho
Nascimento, Maurício
Costa, Rúbia
Silva, Juliana
Renard, Gaby
Guimarães, Luiz Henrique
Penna, Gerson Oliveira
Barral-Netto, Manoel
Carvalho, Lucas P.
Machado, Paulo Roberto Lima
Carvalho, Edgar M.
dc.subject.mesh.pt_BR.fl_str_mv Administration, Oral
Antiprotozoal Agents
Clinical Trials as Topic
Leishmaniasis
topic Administration, Oral
Antiprotozoal Agents
Clinical Trials as Topic
Leishmaniasis
Cutaneous leishmaniasis
Miltefosine
Leishmaniose
Leishmaniose Cutânea
Ensaios Clínicos como Assunto
Resultado do Tratamento
dc.subject.en.pt_BR.fl_str_mv Cutaneous leishmaniasis
Miltefosine
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose
Leishmaniose Cutânea
Ensaios Clínicos como Assunto
Resultado do Tratamento
description Gerson Oliveira Penna - Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento do vinculo com a unidade da Fiocruz Brasília.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-10T15:50:08Z
dc.date.available.fl_str_mv 2020-08-10T15:50:08Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEIXOTO, Fábio et al. Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis. Journal of Immunology Research, [s.l], v. 2020, n. 2789859, p. 1-9, Aug. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42614
dc.identifier.issn.pt_BR.fl_str_mv 2314-7156
dc.identifier.doi.none.fl_str_mv 10.1155/2020/2789859
identifier_str_mv PEIXOTO, Fábio et al. Evaluation of the ability of Miltefosine associated with topical GM-CSF in modulating the immune response of patients with cutaneous Leishmaniasis. Journal of Immunology Research, [s.l], v. 2020, n. 2789859, p. 1-9, Aug. 2020.
2314-7156
10.1155/2020/2789859
url https://www.arca.fiocruz.br/handle/icict/42614
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/42614/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/42614/2/ve_Fabio_Peixoto_etal.pdf
https://www.arca.fiocruz.br/bitstream/icict/42614/3/ve_Fabio_Peixoto_etal.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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