Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43639 |
Resumo: | Brazilian Ministry of Health and the National Council for Scientific and Technological Development (CNPq). |
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Peixoto, FábioNascimento, Maurício T.Costa, RúbiaSilva, JulianaRenard, GabyGuimarães, Luiz HenriquePenna, GersonBarral Netto, ManoelCarvalho, Lucas Pedreira deMachado, Paulo Roberto LimaCarvalho, Edgar Marcelino de2020-09-28T12:58:50Z2020-09-28T12:58:50Z2020PEIXOTO, Fábio et al. Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis. Journal of Immunology Research, 2020.2314-8861https://www.arca.fiocruz.br/handle/icict/43639Brazilian Ministry of Health and the National Council for Scientific and Technological Development (CNPq).Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Quatro G Pesquisa & Desenvolvimento Ltda. Porto Alegre, RS, Brasil.Universidade Federal do Sul da Bahia. Teixeira de Freitas, BA, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Cutaneous leishmaniasis (CL) due to L. braziliensis is associated with an exaggerated inflammatory response and tissue damage. Miltefosine is more effective than pentavalent antimony (Sbv) in the treatment of CL, and here, we evaluate the ability of Sbv, miltefosine, and GM-CSF administered intravenously, orally, or topically, respectively, to modify the immune response. Patients were treated with miltefosine plus GM-CSF, miltefosine plus placebo, or Sbv. Mononuclear cells were stimulated with soluble Leishmania antigen (SLA) on day 0 and day 15 of therapy, and cytokine levels were determined in supernatants by ELISA. The lymphocyte proliferation and oxidative burst were evaluated by flow cytometry, and the degree of infection and Leishmania killing by optical microscopy. Proliferation of CD4+ T cells were enhanced in patients using miltefosine and in CD8+ T cells when GM-CSF was associated. Enhancement in the oxidative burst occurred in the miltefosine plus GM-CSF group on day 15 of therapy. Moreover, the number of L. braziliensis in infected monocytes on day 15 as well as the percentage of infected cells was lower after 48- and 72-hour culture in cells from patients treated with miltefosine plus GM-CSF. In addition to the ability of miltefosine to kill Leishmania, the changes in the immune response caused by miltefosine and GM-CSF may increase the cure rate of CL patients using these drugs.engHindawiLeishmaniose CutâneaInfeccçãoLeishmaniaTratamentoLeishmaniasis CutaneousinfectionLeishmaniaTreatmentEvaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43639/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPeixoto, Fabio Evaluation....pdfPeixoto, Fabio Evaluation....pdfapplication/pdf1330639https://www.arca.fiocruz.br/bitstream/icict/43639/2/Peixoto%2c%20Fabio%20%20Evaluation....pdf6863698cf8466cae1d58f5f10de7471cMD52TEXTPeixoto, Fabio Evaluation....pdf.txtPeixoto, Fabio Evaluation....pdf.txtExtracted texttext/plain40528https://www.arca.fiocruz.br/bitstream/icict/43639/3/Peixoto%2c%20Fabio%20%20Evaluation....pdf.txt7acad5c8a2aa68f3b84ed340e74275d3MD53icict/436392023-03-15 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| dc.title.pt_BR.fl_str_mv |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| title |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| spellingShingle |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis Peixoto, Fábio Leishmaniose Cutânea Infeccção Leishmania Tratamento Leishmaniasis Cutaneous infection Leishmania Treatment |
| title_short |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| title_full |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| title_fullStr |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| title_full_unstemmed |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| title_sort |
Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis |
| author |
Peixoto, Fábio |
| author_facet |
Peixoto, Fábio Nascimento, Maurício T. Costa, Rúbia Silva, Juliana Renard, Gaby Guimarães, Luiz Henrique Penna, Gerson Barral Netto, Manoel Carvalho, Lucas Pedreira de Machado, Paulo Roberto Lima Carvalho, Edgar Marcelino de |
| author_role |
author |
| author2 |
Nascimento, Maurício T. Costa, Rúbia Silva, Juliana Renard, Gaby Guimarães, Luiz Henrique Penna, Gerson Barral Netto, Manoel Carvalho, Lucas Pedreira de Machado, Paulo Roberto Lima Carvalho, Edgar Marcelino de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Peixoto, Fábio Nascimento, Maurício T. Costa, Rúbia Silva, Juliana Renard, Gaby Guimarães, Luiz Henrique Penna, Gerson Barral Netto, Manoel Carvalho, Lucas Pedreira de Machado, Paulo Roberto Lima Carvalho, Edgar Marcelino de |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Cutânea Infeccção Leishmania Tratamento |
| topic |
Leishmaniose Cutânea Infeccção Leishmania Tratamento Leishmaniasis Cutaneous infection Leishmania Treatment |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Cutaneous infection Leishmania Treatment |
| description |
Brazilian Ministry of Health and the National Council for Scientific and Technological Development (CNPq). |
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2020 |
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2020-09-28T12:58:50Z |
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2020-09-28T12:58:50Z |
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2020 |
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PEIXOTO, Fábio et al. Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis. Journal of Immunology Research, 2020. |
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https://www.arca.fiocruz.br/handle/icict/43639 |
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2314-8861 |
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PEIXOTO, Fábio et al. Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis. Journal of Immunology Research, 2020. 2314-8861 |
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