In vitro differentiation of Trypanosoma cruzi under chemically defined conditions

Detalhes bibliográficos
Autor(a) principal: Contreras, Victor T.
Data de Publicação: 1985
Outros Autores: Salles, Jussara M., Thomas, Neide, Morel, Carlos Medicis, Goldenberg, Samuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14681
Resumo: Universidad de Carabobo. Valencia, Venezuela.
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spelling Contreras, Victor T.Salles, Jussara M.Thomas, NeideMorel, Carlos MedicisGoldenberg, Samuel2016-06-28T15:57:55Z2016-07-07T12:15:51Z2016-06-28T15:57:55Z2016-07-07T12:15:51Z1985CONTRERAS, Victor T. et al. In vitro differentatiation of Trypanosoma Cruzi under chemically defined conditions. Molecular and Biochemical Parasitology, v.16, p.315-327 , 1985.0166-6851https://www.arca.fiocruz.br/handle/icict/146811872-9428engElsevierTrypanosoma cruziMetacyclogenesisIn vitro differentiationTrypanosoma cruziIn vitro differentiation of Trypanosoma cruzi under chemically defined conditionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de Carabobo. Valencia, Venezuela.Universidade Federal Fluminense. Niteroi, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Metacyclic trypomastigotes of Trypanosoma cruzi have been obtained in chemically defined axenic culture. The differentiating medium, composed of artificial triatomine urine supplemented with proline, allows high yields of metacyclic trypomastigotes after 72-h incubation of T. cruzi cells at 27°C. Morphological differentiation of the parasites is gradual under these chemically defined conditions and is preceded by the expression of stage-specific polypeptides. The yield of in vitro-induced metacyclic trypomastigotes depends upon the age of the epimastigote culture, the size of the inoculum and the depth of the medium. Metacyclic trypomastigotes differentiated in vitro from the Dm 28c clone of T. cruzi are both resistant to complement lysis and to macrophage digestion. They are able to infect mice with an efficiency similar to that obtained for natural metacyclic trypomastigotes obtained from triatomine excreta.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos4_morel_etal_IOC_1985.pdf.txtcarlos4_morel_etal_IOC_1985.pdf.txtExtracted texttext/plain31338https://www.arca.fiocruz.br/bitstream/icict/14681/4/carlos4_morel_etal_IOC_1985.pdf.txt5af835d09ae59be8fbc6dbf8d77ad7eaMD54ORIGINALcarlos4_morel_etal_IOC_1985.pdfapplication/pdf1403149https://www.arca.fiocruz.br/bitstream/icict/14681/2/carlos4_morel_etal_IOC_1985.pdfd720bd36b8c8382c90dc6b7c0c157aa0MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14681/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146812022-06-24 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dc.title.pt_BR.fl_str_mv In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
title In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
spellingShingle In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
Contreras, Victor T.
Trypanosoma cruzi
Metacyclogenesis
In vitro differentiation
Trypanosoma cruzi
title_short In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
title_full In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
title_fullStr In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
title_full_unstemmed In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
title_sort In vitro differentiation of Trypanosoma cruzi under chemically defined conditions
author Contreras, Victor T.
author_facet Contreras, Victor T.
Salles, Jussara M.
Thomas, Neide
Morel, Carlos Medicis
Goldenberg, Samuel
author_role author
author2 Salles, Jussara M.
Thomas, Neide
Morel, Carlos Medicis
Goldenberg, Samuel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Contreras, Victor T.
Salles, Jussara M.
Thomas, Neide
Morel, Carlos Medicis
Goldenberg, Samuel
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
Metacyclogenesis
In vitro differentiation
Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Metacyclogenesis
In vitro differentiation
Trypanosoma cruzi
description Universidad de Carabobo. Valencia, Venezuela.
publishDate 1985
dc.date.issued.fl_str_mv 1985
dc.date.accessioned.fl_str_mv 2016-06-28T15:57:55Z
2016-07-07T12:15:51Z
dc.date.available.fl_str_mv 2016-06-28T15:57:55Z
2016-07-07T12:15:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CONTRERAS, Victor T. et al. In vitro differentatiation of Trypanosoma Cruzi under chemically defined conditions. Molecular and Biochemical Parasitology, v.16, p.315-327 , 1985.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14681
dc.identifier.issn.pt_BR.fl_str_mv 0166-6851
dc.identifier.eissn.pt_BR.fl_str_mv 1872-9428
identifier_str_mv CONTRERAS, Victor T. et al. In vitro differentatiation of Trypanosoma Cruzi under chemically defined conditions. Molecular and Biochemical Parasitology, v.16, p.315-327 , 1985.
0166-6851
1872-9428
url https://www.arca.fiocruz.br/handle/icict/14681
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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