Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100016 |
Resumo: | The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules. |
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Memórias do Instituto Oswaldo Cruz |
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Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined mediaTrypanosoma cruzimetacyclogenesisChaga's diseasemuscle cellsThe biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules.Instituto Oswaldo Cruz, Ministério da Saúde1988-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100016Memórias do Instituto Oswaldo Cruz v.83 n.1 1988reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761988000100016info:eu-repo/semantics/openAccessContreras,Victor T.Araújo-Jorge,Tania C. deBonaldo,Myrna C.Thomaz,NeideBarbosa,Helena S.Meirelles,Maria de Nazareth S. L. deGoldenberg,Samueleng2020-04-25T17:46:04Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:02:21.218Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
title |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
spellingShingle |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media Contreras,Victor T. Trypanosoma cruzi metacyclogenesis Chaga's disease muscle cells |
title_short |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
title_full |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
title_fullStr |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
title_full_unstemmed |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
title_sort |
Biological aspects of the DM28C clone of Trypanosoma cruzi after metacylogenesis in chemically defined media |
author |
Contreras,Victor T. |
author_facet |
Contreras,Victor T. Araújo-Jorge,Tania C. de Bonaldo,Myrna C. Thomaz,Neide Barbosa,Helena S. Meirelles,Maria de Nazareth S. L. de Goldenberg,Samuel |
author_role |
author |
author2 |
Araújo-Jorge,Tania C. de Bonaldo,Myrna C. Thomaz,Neide Barbosa,Helena S. Meirelles,Maria de Nazareth S. L. de Goldenberg,Samuel |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Contreras,Victor T. Araújo-Jorge,Tania C. de Bonaldo,Myrna C. Thomaz,Neide Barbosa,Helena S. Meirelles,Maria de Nazareth S. L. de Goldenberg,Samuel |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi metacyclogenesis Chaga's disease muscle cells |
topic |
Trypanosoma cruzi metacyclogenesis Chaga's disease muscle cells |
dc.description.none.fl_txt_mv |
The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules. |
description |
The biological characterization of the Trypanosoma cruzi clone Dm 28c in terms of its growth in LIT medium, cell-cycle, infectivity to mice and interaction with professional and non-professional phagocytic cells shows that it behaves as a bona fide T. cruzi representant. The biological properties of this myotropic clone do not change according to the origin of the trypomastigote forms (i. e., from triatomines, infected mice, cell-culture or from the chemically defined TAUP and TAU3AAG media). In addition Dm 28c metacyclic trypomastigotes from TAU3AAG medium display a high infectivity level to fibroblasts and muscle cells. Experiments on binding of cationized ferritin to trypomastigotes surface show the existence of cap-like structures of ferritin in regions near the kinetoplast. However the nature and role of these anionic sites remain to be determined. The results indicate that metacyclic trypomastigotes from Dm 28c clone obtained under chemically defined conditions reproduce the biological behaviour of T. cruzi, rendering this system very suitable for the study of cell-parasite interactions and for the isolation of trypanosome relevant macromolecules. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100016 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761988000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02761988000100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.83 n.1 1988 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
|
_version_ |
1669937651038289920 |