A simple method for human peripheral blood monocyte isolation

Bibliographic Details
Main Author: Almeida, Marcos Cerqueira de
Publication Date: 2000
Other Authors: Silva, Alan C, Barral, Aldina Maria Prado, Barral Netto, Manoel
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/6232
Summary: Universidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, Brasil
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repository_id_str 2135
spelling Almeida, Marcos Cerqueira deSilva, Alan CBarral, Aldina Maria PradoBarral Netto, Manoel2013-01-22T21:12:43Z2013-01-22T21:12:43Z2000ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000.0074-0276https://www.arca.fiocruz.br/handle/icict/6232engA simple method for human peripheral blood monocyte isolationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilWe describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.MonocytesPercollGradientIsolationSeparação Celular/métodosCentrifugação com Gradiente deConcentração/métodosMonócitosHumanosResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAlmeida, Marcos C. A simple method....pdfAlmeida, Marcos C. A simple method....pdfapplication/pdf46739https://www.arca.fiocruz.br/bitstream/icict/6232/1/Almeida%2c%20Marcos%20C.%20A%20simple%20method....pdf50c9b5e7870e3bd3508051c5d753ec3fMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6232/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAlmeida, Marcos C. A simple method....pdf.txtAlmeida, Marcos C. A simple method....pdf.txtExtracted texttext/plain10678https://www.arca.fiocruz.br/bitstream/icict/6232/5/Almeida%2c%20Marcos%20C.%20A%20simple%20method....pdf.txt851a1044abd11162f4b69f7507e06688MD55THUMBNAILAlmeida, Marcos C. A simple method....pdf.jpgAlmeida, Marcos C. 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dc.title.pt_BR.fl_str_mv A simple method for human peripheral blood monocyte isolation
title A simple method for human peripheral blood monocyte isolation
spellingShingle A simple method for human peripheral blood monocyte isolation
Almeida, Marcos Cerqueira de
Monocytes
Percoll
Gradient
Isolation
Separação Celular/métodos
Centrifugação com Gradiente de
Concentração/métodos
Monócitos
Humanos
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
title_short A simple method for human peripheral blood monocyte isolation
title_full A simple method for human peripheral blood monocyte isolation
title_fullStr A simple method for human peripheral blood monocyte isolation
title_full_unstemmed A simple method for human peripheral blood monocyte isolation
title_sort A simple method for human peripheral blood monocyte isolation
author Almeida, Marcos Cerqueira de
author_facet Almeida, Marcos Cerqueira de
Silva, Alan C
Barral, Aldina Maria Prado
Barral Netto, Manoel
author_role author
author2 Silva, Alan C
Barral, Aldina Maria Prado
Barral Netto, Manoel
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida, Marcos Cerqueira de
Silva, Alan C
Barral, Aldina Maria Prado
Barral Netto, Manoel
dc.subject.en.pt_BR.fl_str_mv Monocytes
Percoll
Gradient
Isolation
topic Monocytes
Percoll
Gradient
Isolation
Separação Celular/métodos
Centrifugação com Gradiente de
Concentração/métodos
Monócitos
Humanos
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
dc.subject.decs.pt_BR.fl_str_mv Separação Celular/métodos
Centrifugação com Gradiente de
Concentração/métodos
Monócitos
Humanos
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
description Universidade de Brasília. Faculdade de Ciências Sociais. Departamento de Patologia. Brasília, DF, Brasil
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2013-01-22T21:12:43Z
dc.date.available.fl_str_mv 2013-01-22T21:12:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6232
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv ALMEIDA, M. C. de et al. A simple method for human peripheral blood monocyte isolation. Memórias do Instituto Oswaldo Cruz, v. 95, n. 2, p. 221-223, mar.-apr. 2000.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/6232
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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