A simple method for human peripheral blood monocyte Isolation

Detalhes bibliográficos
Autor(a) principal: Almeida, Marcos C de
Data de Publicação: 2000
Outros Autores: Silva, Alan C, Barral, Aldina, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/25587
https://dx.doi.org/10.1590/S0074-02762000000200014
Resumo: We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
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spelling Almeida, Marcos C deSilva, Alan CBarral, AldinaBarral Netto, Manoel2017-12-07T04:32:47Z2017-12-07T04:32:47Z2000Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000http://repositorio.unb.br/handle/10482/25587https://dx.doi.org/10.1590/S0074-02762000000200014We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.Em processamentoInstituto Oswaldo Cruz, Ministério da SaúdeA simple method for human peripheral blood monocyte Isolationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlemonocytespercollgradient-isolationinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINAL3879.pdfapplication/pdf46739http://repositorio2.unb.br/jspui/bitstream/10482/25587/1/3879.pdf50c9b5e7870e3bd3508051c5d753ec3fMD51open access10482/255872023-10-09 17:55:47.467open accessoai:repositorio2.unb.br:10482/25587Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2023-10-09T20:55:47Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.pt_BR.fl_str_mv A simple method for human peripheral blood monocyte Isolation
title A simple method for human peripheral blood monocyte Isolation
spellingShingle A simple method for human peripheral blood monocyte Isolation
Almeida, Marcos C de
monocytes
percoll
gradient-isolation
title_short A simple method for human peripheral blood monocyte Isolation
title_full A simple method for human peripheral blood monocyte Isolation
title_fullStr A simple method for human peripheral blood monocyte Isolation
title_full_unstemmed A simple method for human peripheral blood monocyte Isolation
title_sort A simple method for human peripheral blood monocyte Isolation
author Almeida, Marcos C de
author_facet Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
author_role author
author2 Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
author2_role author
author
author
dc.contributor.author.fl_str_mv Almeida, Marcos C de
Silva, Alan C
Barral, Aldina
Barral Netto, Manoel
dc.subject.keyword.pt_BR.fl_str_mv monocytes
percoll
gradient-isolation
topic monocytes
percoll
gradient-isolation
description We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.
publishDate 2000
dc.date.issued.fl_str_mv 2000
dc.date.accessioned.fl_str_mv 2017-12-07T04:32:47Z
dc.date.available.fl_str_mv 2017-12-07T04:32:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000
dc.identifier.uri.fl_str_mv http://repositorio.unb.br/handle/10482/25587
dc.identifier.doi.pt_BR.fl_str_mv https://dx.doi.org/10.1590/S0074-02762000000200014
identifier_str_mv Mem. Inst. Oswaldo Cruz,v.95,n.2,p.221-223,2000
url http://repositorio.unb.br/handle/10482/25587
https://dx.doi.org/10.1590/S0074-02762000000200014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
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