The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.

Detalhes bibliográficos
Autor(a) principal: Choy, Henry A
Data de Publicação: 2011
Outros Autores: Kelley, Melissa M, Croda, Julio Henrique Rosa, Matsunaga, James, Babbitt, Jane T, Ko, Albert Icksang, Picardeau, Mathieu, Haake, David A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12395
Resumo: Albert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Research Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USA
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spelling Choy, Henry AKelley, Melissa MCroda, Julio Henrique RosaMatsunaga, JamesBabbitt, Jane TKo, Albert IcksangPicardeau, MathieuHaake, David A2015-12-11T13:28:09Z2015-12-11T13:28:09Z2011CHOY, H. A. et al. The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans. PLoS One, v. 6, n. 2, p. e16879, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/1239510.1371/journal.pone.0016879engPublic Library of ScienceThe multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAlbert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Research Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USAResearch Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Grande Dourados. Faculty of Health Sciences. Dourados, MGS, BrasilAlbert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Research Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USAAlbert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Research Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Yale University School of Medicine. Department of Epidemiology and Public Health. Division of Epidemiology of Microbial Diseases. New Haven, Connecticut, USAInstitut Pasteur. Unite de Biologie des Spirochetes. Paris, FranceAlbert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / University of California Los Angeles. Department of Urology and Department of Molecular Genetics, Microbiology, and Immunology. Los Angeles, California, USALeptospirosis is a potentially fatal zoonotic disease in humans and animals caused by pathogenic spirochetes, such as Leptospira interrogans. The mode of transmission is commonly limited to the exposure of mucous membrane or damaged skin to water contaminated by leptospires shed in the urine of carriers, such as rats. Infection occurs during seasonal flooding of impoverished tropical urban habitats with large rat populations, but also during recreational activity in open water, suggesting it is very efficient. LigA and LigB are surface localized proteins in pathogenic Leptospira strains with properties that could facilitate the infection of damaged skin. Their expression is rapidly induced by the increase in osmolarity encountered by leptospires upon transition from water to host. In addition, the immunoglobulin-like repeats of the Lig proteins bind proteins that mediate attachment to host tissue, such as fibronectin, fibrinogen, collagens, laminin, and elastin, some of which are important in cutaneous wound healing and repair. Hemostasis is critical in a fresh injury, where fibrinogen from damaged vasculature mediates coagulation. We show that fibrinogen binding by recombinant LigB inhibits fibrin formation, which could aid leptospiral entry into the circulation, dissemination, and further infection by impairing healing. LigB also binds fibroblast fibronectin and type III collagen, two proteins prevalent in wound repair, thus potentially enhancing leptospiral adhesion to skin openings. LigA or LigB expression by transformation of a nonpathogenic saprophyte, L. biflexa, enhances bacterial adhesion to fibrinogen. Our results suggest that by binding homeostatic proteins found in cutaneous wounds, LigB could facilitate leptospirosis transmission. Both fibronectin and fibrinogen binding have been mapped to an overlapping domain in LigB comprising repeats 9–11, with repeat 11 possibly enhancing binding by a conformational effect. Leptospirosis patient antibodies react with the LigB domain, suggesting applications in diagnosis and vaccines that are currently limited by the strain-specific leptospiral lipopolysaccharide coats.Adesinas Bacterianas/metabolismoFibrinogênio/metabolismoFibronectinas/metabolismoLeptospira interrogans/metabolismoLeptospirose/metabolismoDermatopatias Bacterianas/metabolismoAdesinas Bacterianas/químicaAnticorpos Antibacterianos/imunologiaAderência BacterianaFibrina/metabolismoHumanosLeptospira interrogans/genéticaLeptospirose/microbiologiaLigação ProteicaEstrutura Terciária de ProteínaDermatopatias Bacterianas/microbiologiaTransformação BacterianaCicatrizaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12395/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALChoy HA The multifunctional 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dc.title.pt_BR.fl_str_mv The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
title The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
spellingShingle The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
Choy, Henry A
Adesinas Bacterianas/metabolismo
Fibrinogênio/metabolismo
Fibronectinas/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/metabolismo
Dermatopatias Bacterianas/metabolismo
Adesinas Bacterianas/química
Anticorpos Antibacterianos/imunologia
Aderência Bacteriana
Fibrina/metabolismo
Humanos
Leptospira interrogans/genética
Leptospirose/microbiologia
Ligação Proteica
Estrutura Terciária de Proteína
Dermatopatias Bacterianas/microbiologia
Transformação Bacteriana
Cicatrização
title_short The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
title_full The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
title_fullStr The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
title_full_unstemmed The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
title_sort The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans.
author Choy, Henry A
author_facet Choy, Henry A
Kelley, Melissa M
Croda, Julio Henrique Rosa
Matsunaga, James
Babbitt, Jane T
Ko, Albert Icksang
Picardeau, Mathieu
Haake, David A
author_role author
author2 Kelley, Melissa M
Croda, Julio Henrique Rosa
Matsunaga, James
Babbitt, Jane T
Ko, Albert Icksang
Picardeau, Mathieu
Haake, David A
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Choy, Henry A
Kelley, Melissa M
Croda, Julio Henrique Rosa
Matsunaga, James
Babbitt, Jane T
Ko, Albert Icksang
Picardeau, Mathieu
Haake, David A
dc.subject.decs.pt_BR.fl_str_mv Adesinas Bacterianas/metabolismo
Fibrinogênio/metabolismo
Fibronectinas/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/metabolismo
Dermatopatias Bacterianas/metabolismo
Adesinas Bacterianas/química
Anticorpos Antibacterianos/imunologia
Aderência Bacteriana
Fibrina/metabolismo
Humanos
Leptospira interrogans/genética
Leptospirose/microbiologia
Ligação Proteica
Estrutura Terciária de Proteína
Dermatopatias Bacterianas/microbiologia
Transformação Bacteriana
Cicatrização
topic Adesinas Bacterianas/metabolismo
Fibrinogênio/metabolismo
Fibronectinas/metabolismo
Leptospira interrogans/metabolismo
Leptospirose/metabolismo
Dermatopatias Bacterianas/metabolismo
Adesinas Bacterianas/química
Anticorpos Antibacterianos/imunologia
Aderência Bacteriana
Fibrina/metabolismo
Humanos
Leptospira interrogans/genética
Leptospirose/microbiologia
Ligação Proteica
Estrutura Terciária de Proteína
Dermatopatias Bacterianas/microbiologia
Transformação Bacteriana
Cicatrização
description Albert Einstein College of Medicine. Department of Physiology & Biophysics. Bronx, NY, USA / Research Service. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California, USA
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2015-12-11T13:28:09Z
dc.date.available.fl_str_mv 2015-12-11T13:28:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHOY, H. A. et al. The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans. PLoS One, v. 6, n. 2, p. e16879, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12395
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0016879
identifier_str_mv CHOY, H. A. et al. The multifunctional LigB adhesin binds homeostatic proteins with potential roles in cutaneous infection by pathogenic Leptospira interrogans. PLoS One, v. 6, n. 2, p. e16879, 2011.
1932-6203
10.1371/journal.pone.0016879
url https://www.arca.fiocruz.br/handle/icict/12395
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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