Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil

Detalhes bibliográficos
Autor(a) principal: Barbosa, André Victor
Data de Publicação: 2015
Outros Autores: Cerqueira, Aloysio de Mello Figueiredo, Rusak, Leonardo Alves, Reis, Cristhiane Moura Falavina dos, Leal, Nilma Cintra, Hofer, Ernesto, Vallim, Deyse Christina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13057
Resumo: Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
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spelling Barbosa, André VictorCerqueira, Aloysio de Mello FigueiredoRusak, Leonardo AlvesReis, Cristhiane Moura Falavina dosLeal, Nilma CintraHofer, ErnestoVallim, Deyse Christina2016-03-08T15:15:59Z2016-03-08T15:15:59Z2015BARBOSA, André Victor: et al. Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil. Journal of Infection in Developing Countries, v.9, n.9, p.962-969, 2015.1972-2680https://www.arca.fiocruz.br/handle/icict/1305710.3855/jidc.56392036-6590engOpen Learning on Enteric PathogensCharacterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECIIpositive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.Listeria monocytogenesSerotype 4bEpidemic clonesVirulenceEpidemiologyListeria monocytogenesVirulênciaEpidemiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13057/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALandre_barbosa_etal_IOC_2015.pdfandre_barbosa_etal_IOC_2015.pdfapplication/pdf771307https://www.arca.fiocruz.br/bitstream/icict/13057/2/andre_barbosa_etal_IOC_2015.pdff6af03ce204ee1aef411a720590d5e7cMD52TEXTandre_barbosa_etal_IOC_2015.pdf.txtandre_barbosa_etal_IOC_2015.pdf.txtExtracted texttext/plain32673https://www.arca.fiocruz.br/bitstream/icict/13057/3/andre_barbosa_etal_IOC_2015.pdf.txtce6b74796d72d1f8b3036e09acb017b2MD53icict/130572018-04-02 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dc.title.pt_BR.fl_str_mv Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
title Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
spellingShingle Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
Barbosa, André Victor
Listeria monocytogenes
Serotype 4b
Epidemic clones
Virulence
Epidemiology
Listeria monocytogenes
Virulência
Epidemiologia
title_short Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
title_full Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
title_fullStr Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
title_full_unstemmed Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
title_sort Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
author Barbosa, André Victor
author_facet Barbosa, André Victor
Cerqueira, Aloysio de Mello Figueiredo
Rusak, Leonardo Alves
Reis, Cristhiane Moura Falavina dos
Leal, Nilma Cintra
Hofer, Ernesto
Vallim, Deyse Christina
author_role author
author2 Cerqueira, Aloysio de Mello Figueiredo
Rusak, Leonardo Alves
Reis, Cristhiane Moura Falavina dos
Leal, Nilma Cintra
Hofer, Ernesto
Vallim, Deyse Christina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, André Victor
Cerqueira, Aloysio de Mello Figueiredo
Rusak, Leonardo Alves
Reis, Cristhiane Moura Falavina dos
Leal, Nilma Cintra
Hofer, Ernesto
Vallim, Deyse Christina
dc.subject.en.pt_BR.fl_str_mv Listeria monocytogenes
Serotype 4b
Epidemic clones
Virulence
Epidemiology
topic Listeria monocytogenes
Serotype 4b
Epidemic clones
Virulence
Epidemiology
Listeria monocytogenes
Virulência
Epidemiologia
dc.subject.decs.pt_BR.fl_str_mv Listeria monocytogenes
Virulência
Epidemiologia
description Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Enteropatógenos, Microbiologia Veterinária e de Alimentos. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-08T15:15:59Z
dc.date.available.fl_str_mv 2016-03-08T15:15:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARBOSA, André Victor: et al. Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil. Journal of Infection in Developing Countries, v.9, n.9, p.962-969, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13057
dc.identifier.issn.none.fl_str_mv 1972-2680
dc.identifier.doi.none.fl_str_mv 10.3855/jidc.5639
dc.identifier.eissn.none.fl_str_mv 2036-6590
identifier_str_mv BARBOSA, André Victor: et al. Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil. Journal of Infection in Developing Countries, v.9, n.9, p.962-969, 2015.
1972-2680
10.3855/jidc.5639
2036-6590
url https://www.arca.fiocruz.br/handle/icict/13057
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Open Learning on Enteric Pathogens
publisher.none.fl_str_mv Open Learning on Enteric Pathogens
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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