Molecular characterization of Listeria monocytogenes isolated from foods
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista de Microbiologia |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011 |
Resumo: | A total of 30 strains of Listeria monocytogenes isolated from different foods (16 of differents kinds of sausage, 14 cheese,) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phosphalipase C - PI-PLC enzyme. The L. monocytogenes strains were differentiated into six ribotype classes. A total of 13 (43.3%) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard L. monocytogenes prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7%) strains when incubated at 37oC, but not at 4oC. The direct colony hybridization method for hemolysin gene detection showed a positive reaction whit all the 30 L. monocytogenes strains, while showed negative reaction with other Listeria spp. The PI-PLC was produced by 27 (90%) of the strains analysed. There was no correlation between the six identified ribotypes and the virulence factors (hemolysin and PI-PLC) studied. |
id |
SBM-2_6e05edef8f94a08d535f3332125a43d0 |
---|---|
oai_identifier_str |
oai:scielo:S0001-37141999000400011 |
network_acronym_str |
SBM-2 |
network_name_str |
Revista de Microbiologia |
repository_id_str |
|
spelling |
Molecular characterization of Listeria monocytogenes isolated from foodsListeria monocytogenesribotypingvirulencefactorsfoodA total of 30 strains of Listeria monocytogenes isolated from different foods (16 of differents kinds of sausage, 14 cheese,) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phosphalipase C - PI-PLC enzyme. The L. monocytogenes strains were differentiated into six ribotype classes. A total of 13 (43.3%) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard L. monocytogenes prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7%) strains when incubated at 37oC, but not at 4oC. The direct colony hybridization method for hemolysin gene detection showed a positive reaction whit all the 30 L. monocytogenes strains, while showed negative reaction with other Listeria spp. The PI-PLC was produced by 27 (90%) of the strains analysed. There was no correlation between the six identified ribotypes and the virulence factors (hemolysin and PI-PLC) studied.Sociedade Brasileira de Microbiologia1999-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011Revista de Microbiologia v.30 n.4 1999reponame:Revista de Microbiologiainstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S0001-37141999000400011info:eu-repo/semantics/openAccessPimenta,Fabiana CristinaFurlanetto,Sirdéia Maura PerroneMayer,Leonard W.Timenetsky,JorgeSantos,Manoel Armando Azevedo doseng2000-04-19T00:00:00Zoai:scielo:S0001-37141999000400011Revistahttps://www.scielo.br/j/rm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||revmicro@icb.usp.br0001-37140001-3714opendoar:2000-04-19T00:00Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Molecular characterization of Listeria monocytogenes isolated from foods |
title |
Molecular characterization of Listeria monocytogenes isolated from foods |
spellingShingle |
Molecular characterization of Listeria monocytogenes isolated from foods Pimenta,Fabiana Cristina Listeria monocytogenes ribotyping virulence factors food |
title_short |
Molecular characterization of Listeria monocytogenes isolated from foods |
title_full |
Molecular characterization of Listeria monocytogenes isolated from foods |
title_fullStr |
Molecular characterization of Listeria monocytogenes isolated from foods |
title_full_unstemmed |
Molecular characterization of Listeria monocytogenes isolated from foods |
title_sort |
Molecular characterization of Listeria monocytogenes isolated from foods |
author |
Pimenta,Fabiana Cristina |
author_facet |
Pimenta,Fabiana Cristina Furlanetto,Sirdéia Maura Perrone Mayer,Leonard W. Timenetsky,Jorge Santos,Manoel Armando Azevedo dos |
author_role |
author |
author2 |
Furlanetto,Sirdéia Maura Perrone Mayer,Leonard W. Timenetsky,Jorge Santos,Manoel Armando Azevedo dos |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pimenta,Fabiana Cristina Furlanetto,Sirdéia Maura Perrone Mayer,Leonard W. Timenetsky,Jorge Santos,Manoel Armando Azevedo dos |
dc.subject.por.fl_str_mv |
Listeria monocytogenes ribotyping virulence factors food |
topic |
Listeria monocytogenes ribotyping virulence factors food |
description |
A total of 30 strains of Listeria monocytogenes isolated from different foods (16 of differents kinds of sausage, 14 cheese,) purchased at groceries of São Paulo City were ribotyped and analysed for the presence and expression of hemolysin gene and production of phosphatidylinositol-specific phosphalipase C - PI-PLC enzyme. The L. monocytogenes strains were differentiated into six ribotype classes. A total of 13 (43.3%) from these strains belong to the same ribotype (ribotype I), and was coincident to the ribotype of the standard L. monocytogenes prototype strain (ATCC-15313). The hemolytic activity was observed in 29 (96.7%) strains when incubated at 37oC, but not at 4oC. The direct colony hybridization method for hemolysin gene detection showed a positive reaction whit all the 30 L. monocytogenes strains, while showed negative reaction with other Listeria spp. The PI-PLC was produced by 27 (90%) of the strains analysed. There was no correlation between the six identified ribotypes and the virulence factors (hemolysin and PI-PLC) studied. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0001-37141999000400011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Revista de Microbiologia v.30 n.4 1999 reponame:Revista de Microbiologia instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Revista de Microbiologia |
collection |
Revista de Microbiologia |
repository.name.fl_str_mv |
Revista de Microbiologia - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||revmicro@icb.usp.br |
_version_ |
1754821030233243648 |