Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action

Detalhes bibliográficos
Autor(a) principal: Simões, Letícia S.
Data de Publicação: 2021
Outros Autores: Alves, Quiara L., Camargo, Samuel B., Araújo, Fênix A., Hora, Viviane R. S., Jesus, Rafael L. C., Barreto, Breno C., Macambira, Simone G., Soares, Milena B. P., Meira, Cássio S., Aguiar, Márcio C., Couto, Ricardo D., Lomonte, Bruno, Menezes Filho, José Evaldo, Cruz, Jader S., Santos, Marcos A. Vannier, Silva, Luciana S. Casais e, Silva, Darizy F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45498
Resumo: Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.
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spelling Simões, Letícia S.Alves, Quiara L.Camargo, Samuel B.Araújo, Fênix A.Hora, Viviane R. S.Jesus, Rafael L. C.Barreto, Breno C.Macambira, Simone G.Soares, Milena B. P.Meira, Cássio S.Aguiar, Márcio C.Couto, Ricardo D.Lomonte, BrunoMenezes Filho, José EvaldoCruz, Jader S.Santos, Marcos A. VannierSilva, Luciana S. Casais eSilva, Darizy F.2021-01-04T17:02:40Z2021-01-04T17:02:40Z2021SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021.0378-4274https://www.arca.fiocruz.br/handle/icict/4549810.1016/j.toxlet.2020.11.019engElsevierCrotalus durissus cascavellaCardiotoxicidadeFibrilaçãoCrotalus durissus cascavellaCardiotoxicityAtrialApêndice AtrialCardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of actioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Departamento de Bioquímica e Biofísica. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biomorfologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Análise Clínica e Toxicológica. Salvador, BA, Brasil.Universidade da Costa Rica. Faculdade de Microbiologia. Instituto Clodomiro Picado. San José, Costa Rica.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.Envenoming, resulting from snake bites, is a global public health problem. The present study was undertaken to investigate the influence of Crotalus durissus cascavella (Cdcas) venom on cardiac activity and the mechanisms of action underlying its effect. To investigate the inotropic and chronotropic effects induced by Cdcas, studies were performed on the left and right atria. A series of tests were conducted to investigate whether the negative inotropic effect, induced by Cdcas, was related to cardiac damage. Cdcas venom (0.1-30 μg/mL) elicited a significant negative inotropic effect. The addition of Cdcas crude venom (7.5, 15 and 30 μg/mL) did not induce significant alterations in cell proliferation, nor in the enzymatic activity of total-CK and CKMB. Ultrastructural evaluation demonstrated that cardiac cells from isoproterenol and Cdcas groups revealed discreet swelling and displaced intermyofibrillar mitochondria with disorganization of the cristae. No change was observed in cardiac electrical activity in perfused isolated rat hearts with Cdcas. In addition, Cdcas reduced contractility in isolated cardiomyocytes from the rat left ventricle. The negative inotropic effect of Cdcas was reduced by l-NAME (100 μM), PTIO (100 μM), ODQ (10 μM) and KT5823 (1 μM), suggesting the participation of NO/cGMP/PKG pathway due to Cdcas. In non-anesthetized rats, Cdcas induced hypotension followed by bradycardia, the latter was also observed by ECG (anesthetized animals). Our results suggest that the negative inotropic effect induced by Cdcas venom is unrelated to cardiac toxicity, at least, at the concentrations tested; and occurs through of NO/cGMP/PKG pathway, likely leading to hypotension and bradycardia when administered in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45498/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosVannier_santos_etal_IOC_2020.pdfMarcosVannier_santos_etal_IOC_2020.pdfapplication/pdf5333824https://www.arca.fiocruz.br/bitstream/icict/45498/2/MarcosVannier_santos_etal_IOC_2020.pdf5206cea0145df8695ab7601a3a488dceMD52TEXTMarcosVannier_santos_etal_IOC_2020.pdf.txtMarcosVannier_santos_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
title Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
spellingShingle Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
Simões, Letícia S.
Crotalus durissus cascavella
Cardiotoxicidade
Fibrilação
Crotalus durissus cascavella
Cardiotoxicity
Atrial
Apêndice Atrial
title_short Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
title_full Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
title_fullStr Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
title_full_unstemmed Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
title_sort Cardiac effect induced by Crotalus durissus cascavella venom: morphological evidence and mechanism of action
author Simões, Letícia S.
author_facet Simões, Letícia S.
Alves, Quiara L.
Camargo, Samuel B.
Araújo, Fênix A.
Hora, Viviane R. S.
Jesus, Rafael L. C.
Barreto, Breno C.
Macambira, Simone G.
Soares, Milena B. P.
Meira, Cássio S.
Aguiar, Márcio C.
Couto, Ricardo D.
Lomonte, Bruno
Menezes Filho, José Evaldo
Cruz, Jader S.
Santos, Marcos A. Vannier
Silva, Luciana S. Casais e
Silva, Darizy F.
author_role author
author2 Alves, Quiara L.
Camargo, Samuel B.
Araújo, Fênix A.
Hora, Viviane R. S.
Jesus, Rafael L. C.
Barreto, Breno C.
Macambira, Simone G.
Soares, Milena B. P.
Meira, Cássio S.
Aguiar, Márcio C.
Couto, Ricardo D.
Lomonte, Bruno
Menezes Filho, José Evaldo
Cruz, Jader S.
Santos, Marcos A. Vannier
Silva, Luciana S. Casais e
Silva, Darizy F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Simões, Letícia S.
Alves, Quiara L.
Camargo, Samuel B.
Araújo, Fênix A.
Hora, Viviane R. S.
Jesus, Rafael L. C.
Barreto, Breno C.
Macambira, Simone G.
Soares, Milena B. P.
Meira, Cássio S.
Aguiar, Márcio C.
Couto, Ricardo D.
Lomonte, Bruno
Menezes Filho, José Evaldo
Cruz, Jader S.
Santos, Marcos A. Vannier
Silva, Luciana S. Casais e
Silva, Darizy F.
dc.subject.other.pt_BR.fl_str_mv Crotalus durissus cascavella
Cardiotoxicidade
Fibrilação
topic Crotalus durissus cascavella
Cardiotoxicidade
Fibrilação
Crotalus durissus cascavella
Cardiotoxicity
Atrial
Apêndice Atrial
dc.subject.en.pt_BR.fl_str_mv Crotalus durissus cascavella
Cardiotoxicity
Atrial
dc.subject.decs.pt_BR.fl_str_mv Apêndice Atrial
description Universidade Federal da Bahia. Departamento de Bioregulação. Salvador, BA, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-01-04T17:02:40Z
dc.date.available.fl_str_mv 2021-01-04T17:02:40Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45498
dc.identifier.issn.pt_BR.fl_str_mv 0378-4274
dc.identifier.doi.none.fl_str_mv 10.1016/j.toxlet.2020.11.019
identifier_str_mv SIMÕES, Letícia O. et al. Cardiac effect induced by Crotalus durissus venom: Morphofunctional evidence and mechanism of action. Toxicology Letters, v. 337, p. 121-133, 2021.
0378-4274
10.1016/j.toxlet.2020.11.019
url https://www.arca.fiocruz.br/handle/icict/45498
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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