New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37797 |
Resumo: | Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), Conselho Nacional de Pesquisa (CNPq), and Decanato de Pesquisa e Pós-Graduação da Universidade de Brasília (DPP/UnB) for financial support, and CAPES for graduate students’ grants. |
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Souza, Ana Camila OliveiraFavali, CecíliaSoares, Naiara CarolineTavares, Natalia MachadoJerônimo, Márcio SousaVeloso Junior, Paulo HenriqueMarina, Clara LunaSantos, ClaireBrodskyn, Cláudia IdaBocca, Anamelia Lorenzetti2019-12-10T17:15:42Z2019-12-10T17:15:42Z2019SOUZA, Ana Camila Oliveira et al. New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells. Frontiers in Microbiology, v. 10, p. 1-9, Oct. 2019.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3779710.3389/fmicb.2019.02445Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), Conselho Nacional de Pesquisa (CNPq), and Decanato de Pesquisa e Pós-Graduação da Universidade de Brasília (DPP/UnB) for financial support, and CAPES for graduate students’ grants.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade de Brasília. Departamento de Biologia Celular. Brasília, DF, Brasil.The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated the influence of two cell wall fractions of the dimorphic fungi Paracoccidioides brasiliensis (Pb) in the in vitro generation of monocyte-derived dendritic cells (MoDCs). Monocytes were purified from the peripheral blood of healthy donors and cultivated for 7 days in medium supplemented with IL-4 and GM-CSF in the presence of Pb cell wall fractions: the alkali-insoluble F1, constituted by β-1,3-glucans, chitin and proteins, and the alkali-soluble F2, mainly constituted by α-glucan. MoDCs phenotypes were evaluated regarding cell surface expression of CD1a, DC-SIGN, HLA-DR, CD80, and CD83 and production of cytokines. The α-glucan-rich cell wall fraction downregulated the differentiation of CD1a+ MoDCs, a dendritic cell subset that stimulate Th1 responses. The presence of both cell fractions inhibited DC-SIGN and HLA-DR expression, while the expression of maturation markers was differentially induced in CD1a- MoDCs. Differentiation upon F1 and F2 stimulation induced mixed profile of inflammatory cytokines. Altogether, these data demonstrate that Pb cell wall fractions differentially induce a dysregulation in DCs differentiation. Moreover, our results suggest that cell wall α-glucan promote the differentiation of CD1a- DCs, potentially favoring Th2 polarization and contributing to pathogen persistence.engFrontiers MediaParacoccidiodomicoseParacoccidioides brasiliensisDiferenciação celular dendríticaGlucanoFrações da parede celularParacoccidiodomycosisParacoccidioides brasiliensisDendritic cell differentiationGlucanCell wallNew Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37797/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSouza,A.C.O. New Role.pdfSouza,A.C.O. 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| dc.title.pt_BR.fl_str_mv |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| title |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| spellingShingle |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells Souza, Ana Camila Oliveira Paracoccidiodomicose Paracoccidioides brasiliensis Diferenciação celular dendrítica Glucano Frações da parede celular Paracoccidiodomycosis Paracoccidioides brasiliensis Dendritic cell differentiation Glucan Cell wall |
| title_short |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| title_full |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| title_fullStr |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| title_full_unstemmed |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| title_sort |
New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells |
| author |
Souza, Ana Camila Oliveira |
| author_facet |
Souza, Ana Camila Oliveira Favali, Cecília Soares, Naiara Caroline Tavares, Natalia Machado Jerônimo, Márcio Sousa Veloso Junior, Paulo Henrique Marina, Clara Luna Santos, Claire Brodskyn, Cláudia Ida Bocca, Anamelia Lorenzetti |
| author_role |
author |
| author2 |
Favali, Cecília Soares, Naiara Caroline Tavares, Natalia Machado Jerônimo, Márcio Sousa Veloso Junior, Paulo Henrique Marina, Clara Luna Santos, Claire Brodskyn, Cláudia Ida Bocca, Anamelia Lorenzetti |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Ana Camila Oliveira Favali, Cecília Soares, Naiara Caroline Tavares, Natalia Machado Jerônimo, Márcio Sousa Veloso Junior, Paulo Henrique Marina, Clara Luna Santos, Claire Brodskyn, Cláudia Ida Bocca, Anamelia Lorenzetti |
| dc.subject.other.pt_BR.fl_str_mv |
Paracoccidiodomicose Paracoccidioides brasiliensis Diferenciação celular dendrítica Glucano Frações da parede celular |
| topic |
Paracoccidiodomicose Paracoccidioides brasiliensis Diferenciação celular dendrítica Glucano Frações da parede celular Paracoccidiodomycosis Paracoccidioides brasiliensis Dendritic cell differentiation Glucan Cell wall |
| dc.subject.en.pt_BR.fl_str_mv |
Paracoccidiodomycosis Paracoccidioides brasiliensis Dendritic cell differentiation Glucan Cell wall |
| description |
Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), Conselho Nacional de Pesquisa (CNPq), and Decanato de Pesquisa e Pós-Graduação da Universidade de Brasília (DPP/UnB) for financial support, and CAPES for graduate students’ grants. |
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2019 |
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2019-12-10T17:15:42Z |
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2019-12-10T17:15:42Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SOUZA, Ana Camila Oliveira et al. New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells. Frontiers in Microbiology, v. 10, p. 1-9, Oct. 2019. |
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https://www.arca.fiocruz.br/handle/icict/37797 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2019.02445 |
| identifier_str_mv |
SOUZA, Ana Camila Oliveira et al. New Role of P. brasiliensis α-Glucan: Differentiation of Non-conventional Dendritic Cells. Frontiers in Microbiology, v. 10, p. 1-9, Oct. 2019. 1664-302X 10.3389/fmicb.2019.02445 |
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Frontiers Media |
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Frontiers Media |
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