NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.

Detalhes bibliográficos
Autor(a) principal: Cardillo, Fabíola
Data de Publicação: 2004
Outros Autores: Nomizo, Auro, Postól, Edilberto, Mengele Junior, José Orivaldo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10908
Resumo: University of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, Brasil
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spelling Cardillo, FabíolaNomizo, AuroPostól, EdilbertoMengele Junior, José Orivaldo2015-06-18T13:03:27Z2015-06-18T13:03:27Z2004CARDILLO, F. et al. NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection. Medical Science Monitor, v. 10, n. 8, p. BR259-267, 2004.1234-1010https://www.arca.fiocruz.br/handle/icict/10908engMedical Science International PublishingNK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, BrasilUniversity of São Paulo. Department of Clinical Analysis. São Paulo, SP, BrasilUniversity of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, BrasilUniversity of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, BrasilBACKGROUND: This study evaluated the regulatory function of NK1.1+ cells during Trypanosoma cruzi infection. MATERIAL/METHODS: Both thymectomized (Tx C57Bl/6) and euthymic C57Bl/6 mice (C57Bl/6) were infected intraperitoneally with the Tulahuen strain. NK1.1+ cells were depleted in vivo by anti-NK1.1 mAb. Spleen cells were analyzed by flow cytometry for the expression of CD44 and CD69 on T cells. Supernatants from splenocytes were used to measure nitrite concentration (quantified by Griess reagent). Interleukin 2 and IFN-gamma levels were determined by ELISA. The protocols used herein were approved by the Institutional Committee for Ethics. Student's t or Kruskal-Wallis tests were applied, as indicated. RESULTS: The number of T cells expressing CD69 increased progressively during T. cruzi infection in NK1.1 cell-depleted C57Bl/6 mice. In spite of an increased early T cell activation during infection, the percentage of CD4+ CD44high T cells did not augment in NK1.1 cell-depleted C57Bl/6 mice compared with untreated C57Bl/6 controls. Serum levels of IFN-gamma in anti-NK1.1-treated mice were higher than in non-depleted animals. Con-A-stimulated spleen cell supernatants from NK1.1 cell-depleted animals contained increased levels of IL-2 and nitric oxide (NO) during early infection. CONCLUSIONS: After the first week of infection, NO overproduction and high levels of IFN-gamma in anti-NK1.1-tre-ated C57Bl/6 mice appeared to be related to susceptibility and hyperactivation of peripheral T cells. Finally, this study suggests a novel regulatory function of NK1.1+ cells during T. cruzi infection. Without NK1.1 cells, T lymphocytes are hyperactivated but do not differentiate to effector/memory T cells in infected C57Bl/6 mice.T. cruziNK1.1 T cellsRegulatory cellsIFN-gammaNitric oxideInterleukin-2Antígenos CD44/metabolismoAntígenos de Diferenciação de Linfócitos T/metabolismoCélulas Matadoras Naturais/imunologiaBaço/imunologiaLinfócitos T/imunologiaAnimaisCitometria de FluxoInterferon gama/metabolismoInterleucina-2/metabolismoLectinas Tipo CCamundongosCamundongos Endogâmicos C57BLÓxido Nítrico/metabolismoBaço/parasitologiaTrypanosoma cruzi/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/10908/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALCardillo F NK! 1 are required....pdfCardillo F NK! 1 are required....pdfapplication/pdf1290378https://www.arca.fiocruz.br/bitstream/icict/10908/2/Cardillo%20F%20NK%21%201%20are%20required....pdf13c8e37095d5f8e755e95fbabbf02a6bMD52TEXTCardillo F NK! 1 are required....pdf.txtCardillo F NK! 1 are required....pdf.txtExtracted texttext/plain46972https://www.arca.fiocruz.br/bitstream/icict/10908/3/Cardillo%20F%20NK%21%201%20are%20required....pdf.txtdbdc2a27cb58865991ab33a45e33384aMD53icict/109082023-03-15 14:33:00.446oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
title NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
spellingShingle NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
Cardillo, Fabíola
T. cruzi
NK1.1 T cells
Regulatory cells
IFN-gamma
Nitric oxide
Interleukin-2
Antígenos CD44/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Células Matadoras Naturais/imunologia
Baço/imunologia
Linfócitos T/imunologia
Animais
Citometria de Fluxo
Interferon gama/metabolismo
Interleucina-2/metabolismo
Lectinas Tipo C
Camundongos
Camundongos Endogâmicos C57BL
Óxido Nítrico/metabolismo
Baço/parasitologia
Trypanosoma cruzi/imunologia
title_short NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
title_full NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
title_fullStr NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
title_full_unstemmed NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
title_sort NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection.
author Cardillo, Fabíola
author_facet Cardillo, Fabíola
Nomizo, Auro
Postól, Edilberto
Mengele Junior, José Orivaldo
author_role author
author2 Nomizo, Auro
Postól, Edilberto
Mengele Junior, José Orivaldo
author2_role author
author
author
dc.contributor.author.fl_str_mv Cardillo, Fabíola
Nomizo, Auro
Postól, Edilberto
Mengele Junior, José Orivaldo
dc.subject.en.pt_BR.fl_str_mv T. cruzi
NK1.1 T cells
Regulatory cells
IFN-gamma
Nitric oxide
Interleukin-2
topic T. cruzi
NK1.1 T cells
Regulatory cells
IFN-gamma
Nitric oxide
Interleukin-2
Antígenos CD44/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Células Matadoras Naturais/imunologia
Baço/imunologia
Linfócitos T/imunologia
Animais
Citometria de Fluxo
Interferon gama/metabolismo
Interleucina-2/metabolismo
Lectinas Tipo C
Camundongos
Camundongos Endogâmicos C57BL
Óxido Nítrico/metabolismo
Baço/parasitologia
Trypanosoma cruzi/imunologia
dc.subject.decs.pt_BR.fl_str_mv Antígenos CD44/metabolismo
Antígenos de Diferenciação de Linfócitos T/metabolismo
Células Matadoras Naturais/imunologia
Baço/imunologia
Linfócitos T/imunologia
Animais
Citometria de Fluxo
Interferon gama/metabolismo
Interleucina-2/metabolismo
Lectinas Tipo C
Camundongos
Camundongos Endogâmicos C57BL
Óxido Nítrico/metabolismo
Baço/parasitologia
Trypanosoma cruzi/imunologia
description University of São Paulo. Institute for Biomedical Sciences IV. Department of Immunology. São Paulo, SP, Brasil / University of São Paulo. Department of Clinical Analysis. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Doença Experimental de Chagas, Imunologia Celular e Auto-imunidade. Salvador, BA, Brasil
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2015-06-18T13:03:27Z
dc.date.available.fl_str_mv 2015-06-18T13:03:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CARDILLO, F. et al. NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection. Medical Science Monitor, v. 10, n. 8, p. BR259-267, 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10908
dc.identifier.issn.none.fl_str_mv 1234-1010
identifier_str_mv CARDILLO, F. et al. NK1.1 cells are required to control T cell hyperactivity during Trypanosoma cruzi infection. Medical Science Monitor, v. 10, n. 8, p. BR259-267, 2004.
1234-1010
url https://www.arca.fiocruz.br/handle/icict/10908
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Medical Science International Publishing
publisher.none.fl_str_mv Medical Science International Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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