Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice

Detalhes bibliográficos
Autor(a) principal: Iborra, Salvador
Data de Publicação: 2008
Outros Autores: Parody, Nuria, Abánades, Daniel Ruiz, Bonay, Pedro, Prates, Deboraci Brito, Novais, Fernanda Oliveira, Barral Netto, Manoel, Alonso, Carlos, Soto, Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7408
Resumo: Instituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, Spain
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spelling Iborra, SalvadorParody, NuriaAbánades, Daniel RuizBonay, PedroPrates, Deboraci BritoNovais, Fernanda OliveiraBarral Netto, ManoelAlonso, CarlosSoto, Manuel2014-03-13T19:19:59Z2014-03-13T19:19:59Z2008IBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008.1286-4579https://www.arca.fiocruz.br/handle/icict/740810.1016/j.micinf.2008.06.002engElsevier Masson SASVaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, SpainUniversidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, SpainIn the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice.We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-g response mediated mainly by CD4þ T cell, albeit a minor contribution of CD8þ T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-g production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine. 2008 Elsevier Masson SAS. All rights reservedLeishmaniaBALB/C miceC57BL/6 miceTh1/Th2 immune responses;Ribosomal proteinsVaccinesLeishmania major/imunologiaVacinas contra LeishmanioseLeishmaniose Cutânea/prevenção & controleOligodesoxirribonucleotídeos/imunologiaProteínas Ribossômicas/imunologiaAnimaisFemininoImunoglobulina G/metabolismoInterferon gama/metabolismoInterleucina-10/metabolismoInterleucina-4/metabolismoLeishmaniose Cutânea/imunologiaCamundongosCamundongos Endogâmicos BALB CCamundongos Endogâmicos C57BLOligodesoxirribonucleotídeos/genéticaOligodesoxirribonucleotídeos/metabolismoProteínas de Protozoários/genéticaProteínas de Protozoários/imunologiaProteínas de Protozoários/metabolismoProteínas Ribossômicas/genéticaProteínas Ribossômicas/metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBorra S Vaccination with....pdfBorra S Vaccination with....pdfapplication/pdf578715https://www.arca.fiocruz.br/bitstream/icict/7408/1/Borra%20S%20Vaccination%20with....pdfe301f276716710b41f51f2b9d13736c5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7408/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBorra S Vaccination with....pdf.txtBorra S Vaccination with....pdf.txtExtracted texttext/plain41490https://www.arca.fiocruz.br/bitstream/icict/7408/5/Borra%20S%20Vaccination%20with....pdf.txte4042f0c81e6136d020b150dca9b9471MD55THUMBNAILBorra S Vaccination with....pdf.jpgBorra S Vaccination with....pdf.jpgGenerated Thumbnailimage/jpeg2352https://www.arca.fiocruz.br/bitstream/icict/7408/4/Borra%20S%20Vaccination%20with....pdf.jpg55bbad107b7531c22a2c69e791f4bb27MD54icict/74082023-03-15 14:33:01.819oai:www.arca.fiocruz.br:icict/7408TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
title Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
spellingShingle Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
Iborra, Salvador
Leishmania
BALB/C mice
C57BL/6 mice
Th1/Th2 immune responses;
Ribosomal proteins
Vaccines
Leishmania major/imunologia
Vacinas contra Leishmaniose
Leishmaniose Cutânea/prevenção & controle
Oligodesoxirribonucleotídeos/imunologia
Proteínas Ribossômicas/imunologia
Animais
Feminino
Imunoglobulina G/metabolismo
Interferon gama/metabolismo
Interleucina-10/metabolismo
Interleucina-4/metabolismo
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Oligodesoxirribonucleotídeos/genética
Oligodesoxirribonucleotídeos/metabolismo
Proteínas de Protozoários/genética
Proteínas de Protozoários/imunologia
Proteínas de Protozoários/metabolismo
Proteínas Ribossômicas/genética
Proteínas Ribossômicas/metabolismo
title_short Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
title_full Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
title_fullStr Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
title_full_unstemmed Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
title_sort Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
author Iborra, Salvador
author_facet Iborra, Salvador
Parody, Nuria
Abánades, Daniel Ruiz
Bonay, Pedro
Prates, Deboraci Brito
Novais, Fernanda Oliveira
Barral Netto, Manoel
Alonso, Carlos
Soto, Manuel
author_role author
author2 Parody, Nuria
Abánades, Daniel Ruiz
Bonay, Pedro
Prates, Deboraci Brito
Novais, Fernanda Oliveira
Barral Netto, Manoel
Alonso, Carlos
Soto, Manuel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Iborra, Salvador
Parody, Nuria
Abánades, Daniel Ruiz
Bonay, Pedro
Prates, Deboraci Brito
Novais, Fernanda Oliveira
Barral Netto, Manoel
Alonso, Carlos
Soto, Manuel
dc.subject.en.pt_BR.fl_str_mv Leishmania
BALB/C mice
C57BL/6 mice
Th1/Th2 immune responses;
Ribosomal proteins
Vaccines
topic Leishmania
BALB/C mice
C57BL/6 mice
Th1/Th2 immune responses;
Ribosomal proteins
Vaccines
Leishmania major/imunologia
Vacinas contra Leishmaniose
Leishmaniose Cutânea/prevenção & controle
Oligodesoxirribonucleotídeos/imunologia
Proteínas Ribossômicas/imunologia
Animais
Feminino
Imunoglobulina G/metabolismo
Interferon gama/metabolismo
Interleucina-10/metabolismo
Interleucina-4/metabolismo
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Oligodesoxirribonucleotídeos/genética
Oligodesoxirribonucleotídeos/metabolismo
Proteínas de Protozoários/genética
Proteínas de Protozoários/imunologia
Proteínas de Protozoários/metabolismo
Proteínas Ribossômicas/genética
Proteínas Ribossômicas/metabolismo
dc.subject.decs.pt_BR.fl_str_mv Leishmania major/imunologia
Vacinas contra Leishmaniose
Leishmaniose Cutânea/prevenção & controle
Oligodesoxirribonucleotídeos/imunologia
Proteínas Ribossômicas/imunologia
Animais
Feminino
Imunoglobulina G/metabolismo
Interferon gama/metabolismo
Interleucina-10/metabolismo
Interleucina-4/metabolismo
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Oligodesoxirribonucleotídeos/genética
Oligodesoxirribonucleotídeos/metabolismo
Proteínas de Protozoários/genética
Proteínas de Protozoários/imunologia
Proteínas de Protozoários/metabolismo
Proteínas Ribossômicas/genética
Proteínas Ribossômicas/metabolismo
description Instituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, Spain
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-03-13T19:19:59Z
dc.date.available.fl_str_mv 2014-03-13T19:19:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv IBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7408
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv 10.1016/j.micinf.2008.06.002
identifier_str_mv IBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008.
1286-4579
10.1016/j.micinf.2008.06.002
url https://www.arca.fiocruz.br/handle/icict/7408
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Masson SAS
publisher.none.fl_str_mv Elsevier Masson SAS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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