Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/36863 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil. |
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Pinto, Patricia MachadoBrito, Cristiana F. A.Passos, Liana K. JannotiTendler, MiriamSimpson, Andrew J. G.2019-11-05T13:43:05Z2019-11-05T13:43:05Z1997PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997.0074-0276https://www.arca.fiocruz.br/handle/icict/3686310.1590/s0074-027619970003000191678-9849engFundação Oswaldo Cruz. Instituto Oswaldo CruzSchistosoma mansoniReação em Cadeia da PolimeraseDNA polimórfico amplificado aleatoriamenteEsquistossomosePolymerase chain reactionRandomly amplified polymorphic DNASchistosomiasisSchistosoma mansoniContrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malacologia. Belo Horizonte. MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Esquistossomose Experimental. Rio de Janeiro, RJ, Brasil.Instituto Ludwig de Pesquisas sobre o Câncer. Laboratório de Genética de Câncer. São Paulo, SP, Brasil.The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic DNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36863/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPatriciaM_Pinto_etal_IOC_1997.pdfPatriciaM_Pinto_etal_IOC_1997.pdfapplication/pdf271575https://www.arca.fiocruz.br/bitstream/icict/36863/2/PatriciaM_Pinto_etal_IOC_1997.pdf111c5d8d7277866838054bc8191f2500MD52TEXTPatriciaM_Pinto_etal_IOC_1997.pdf.txtPatriciaM_Pinto_etal_IOC_1997.pdf.txtExtracted texttext/plain17197https://www.arca.fiocruz.br/bitstream/icict/36863/3/PatriciaM_Pinto_etal_IOC_1997.pdf.txtc5d55ff02d44c0d32061e9c3ebe7a7ceMD53icict/368632019-12-15 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dc.title.pt_BR.fl_str_mv |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
title |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
spellingShingle |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni Pinto, Patricia Machado Schistosoma mansoni Reação em Cadeia da Polimerase DNA polimórfico amplificado aleatoriamente Esquistossomose Polymerase chain reaction Randomly amplified polymorphic DNA Schistosomiasis Schistosoma mansoni |
title_short |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
title_full |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
title_fullStr |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
title_full_unstemmed |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
title_sort |
Contrasting genomic variability between clones from field isolates and laboratory populations of Schistosoma mansoni |
author |
Pinto, Patricia Machado |
author_facet |
Pinto, Patricia Machado Brito, Cristiana F. A. Passos, Liana K. Jannoti Tendler, Miriam Simpson, Andrew J. G. |
author_role |
author |
author2 |
Brito, Cristiana F. A. Passos, Liana K. Jannoti Tendler, Miriam Simpson, Andrew J. G. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Pinto, Patricia Machado Brito, Cristiana F. A. Passos, Liana K. Jannoti Tendler, Miriam Simpson, Andrew J. G. |
dc.subject.other.pt_BR.fl_str_mv |
Schistosoma mansoni Reação em Cadeia da Polimerase DNA polimórfico amplificado aleatoriamente Esquistossomose |
topic |
Schistosoma mansoni Reação em Cadeia da Polimerase DNA polimórfico amplificado aleatoriamente Esquistossomose Polymerase chain reaction Randomly amplified polymorphic DNA Schistosomiasis Schistosoma mansoni |
dc.subject.en.pt_BR.fl_str_mv |
Polymerase chain reaction Randomly amplified polymorphic DNA Schistosomiasis Schistosoma mansoni |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte. MG, Brasil. |
publishDate |
1997 |
dc.date.issued.fl_str_mv |
1997 |
dc.date.accessioned.fl_str_mv |
2019-11-05T13:43:05Z |
dc.date.available.fl_str_mv |
2019-11-05T13:43:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/36863 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/s0074-02761997000300019 |
dc.identifier.eissn.none.fl_str_mv |
1678-9849 |
identifier_str_mv |
PINTO, Patricia Machado et al. Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 3, p. 409-414, May/June 1997. 0074-0276 10.1590/s0074-02761997000300019 1678-9849 |
url |
https://www.arca.fiocruz.br/handle/icict/36863 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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