Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Reis, Mitermayer Galvão dos
Data de Publicação: 1989
Outros Autores: Kuhns, Janet, Blanton, Ronald Edward, Davis, Alan H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7582
Resumo: Escola de Medicina e Saude Publica. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Reis, Mitermayer Galvão dosKuhns, JanetBlanton, Ronald EdwardDavis, Alan H.2014-05-06T12:13:21Z2014-05-06T12:13:21Z1989REIS, M. G. dos et al. Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular Biochemical and Parasitology, v. 32, n. 2-3, p. 113-119, 1989.0166-6851https://www.arca.fiocruz.br/handle/icict/7582engElsevier Science Publishers B.V.Localization and pattern of expression of a female specific mRNA in Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEscola de Medicina e Saude Publica. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilDivision of Geographic Medicine. Department of Medicine. Maryland, USADivision of Geographic Medicine. Department of Medicine. Maryland, USADivision of Geographic Medicine. Department of Medicine. Maryland, USA / Department of Molecular Biology and Microbiology. Case Western Reserve University and University Hospitals. Cleveland, OH, USATo understand mechanisms involved m sex-specific gene expression in Schistosoma mansoni, a cDNA (fs800) was isolated that hybridized to an 800 nucleotide mRNA present in high levels only in mature female worms. The fs800 cDNA sequence was characterized by two long open reading frames and central stretches of repeated amino acids. Fs800 did not share similarities with other known sequences in computer searches. In situ hybridization, however, revealed that the mRNA corresponding to fs800 was found only in female vitelline cells, suggesting that the product of this gene may be involved in the production or function of eggs. Fs800 is developmentally regulated as expression of this gene is dependent on the maturity of female worms. Furthermore, during in vitro culture, when female worms are known to stop egg production, expression of fs800 selectively ceased.Female specific cDNAIn situ hybridizationGene localizationGene expression;Vitelline cellRNA Mensageiro/genéticaSchistosoma mansoni/genéticaDucto VitelinoAnimaisSequência de BasesSondas de DNAFemininoRegulação da Expressão GênicaOviposiçãoSondas RNACaracteres Sexuaisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALReis MG Localization and Pattern....pdfReis MG Localization and Pattern....pdfapplication/pdf816949https://www.arca.fiocruz.br/bitstream/icict/7582/1/Reis%20MG%20Localization%20and%20Pattern....pdfa4857b106f2b578cac1dff92128509d1MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7582/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTReis MG Localization and Pattern....pdf.txtReis MG Localization and Pattern....pdf.txtExtracted texttext/plain24184https://www.arca.fiocruz.br/bitstream/icict/7582/5/Reis%20MG%20Localization%20and%20Pattern....pdf.txt0eadf7499bc2e95f2f08731e22a5ff4aMD55THUMBNAILReis MG Localization and Pattern....pdf.jpgReis MG Localization and Pattern....pdf.jpgGenerated Thumbnailimage/jpeg2382https://www.arca.fiocruz.br/bitstream/icict/7582/4/Reis%20MG%20Localization%20and%20Pattern....pdf.jpga1fe98ff57522da4678a12cd22eef30fMD54icict/75822023-03-15 14:33:05.819oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
title Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
spellingShingle Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
Reis, Mitermayer Galvão dos
Female specific cDNA
In situ hybridization
Gene localization
Gene expression;
Vitelline cell
RNA Mensageiro/genética
Schistosoma mansoni/genética
Ducto Vitelino
Animais
Sequência de Bases
Sondas de DNA
Feminino
Regulação da Expressão Gênica
Oviposição
Sondas RNA
Caracteres Sexuais
title_short Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
title_full Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
title_fullStr Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
title_full_unstemmed Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
title_sort Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni
author Reis, Mitermayer Galvão dos
author_facet Reis, Mitermayer Galvão dos
Kuhns, Janet
Blanton, Ronald Edward
Davis, Alan H.
author_role author
author2 Kuhns, Janet
Blanton, Ronald Edward
Davis, Alan H.
author2_role author
author
author
dc.contributor.author.fl_str_mv Reis, Mitermayer Galvão dos
Kuhns, Janet
Blanton, Ronald Edward
Davis, Alan H.
dc.subject.en.pt_BR.fl_str_mv Female specific cDNA
In situ hybridization
Gene localization
Gene expression;
Vitelline cell
topic Female specific cDNA
In situ hybridization
Gene localization
Gene expression;
Vitelline cell
RNA Mensageiro/genética
Schistosoma mansoni/genética
Ducto Vitelino
Animais
Sequência de Bases
Sondas de DNA
Feminino
Regulação da Expressão Gênica
Oviposição
Sondas RNA
Caracteres Sexuais
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro/genética
Schistosoma mansoni/genética
Ducto Vitelino
Animais
Sequência de Bases
Sondas de DNA
Feminino
Regulação da Expressão Gênica
Oviposição
Sondas RNA
Caracteres Sexuais
description Escola de Medicina e Saude Publica. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 1989
dc.date.issued.fl_str_mv 1989
dc.date.accessioned.fl_str_mv 2014-05-06T12:13:21Z
dc.date.available.fl_str_mv 2014-05-06T12:13:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, M. G. dos et al. Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular Biochemical and Parasitology, v. 32, n. 2-3, p. 113-119, 1989.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7582
dc.identifier.issn.none.fl_str_mv 0166-6851
identifier_str_mv REIS, M. G. dos et al. Localization and pattern of expression of a female specific mRNA in Schistosoma mansoni. Molecular Biochemical and Parasitology, v. 32, n. 2-3, p. 113-119, 1989.
0166-6851
url https://www.arca.fiocruz.br/handle/icict/7582
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science Publishers B.V.
publisher.none.fl_str_mv Elsevier Science Publishers B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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