Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination

Detalhes bibliográficos
Autor(a) principal: Schwarz, Marcos Gustavo Araujo
Data de Publicação: 2020
Outros Autores: Corrêa, Paloma Rezende, Malaga, Wladimir, Guilhot, Christophe, Lima, Leila Mondonça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43189
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Schwarz, Marcos Gustavo AraujoCorrêa, Paloma RezendeMalaga, WladimirGuilhot, ChristopheLima, Leila Mondonça2020-09-04T19:20:40Z2020-09-04T19:20:40Z2020SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination. Tuberculosis. v. 123, p. 1-4, June 2020.1472-9792https://www.arca.fiocruz.br/handle/icict/4318910.1016/j.tube.2020.101956engElsevierRecombinação homólogaMycobacterium bovis BCG MoreauVacina contra tuberculoseHomologous recombinationMycobacterium bovis BCG MoreauRecombineeringTuberculosis vaccineMycobacterium bovis BCG moreau is naturally deficient in homologous recombinationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Centre National de La Recherche Scientifique. Institut de Pharmacologie et de Biologie Structurale. Toulouse, France.Centre National de La Recherche Scientifique. Institut de Pharmacologie et de Biologie Structurale. Toulouse, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.The ability to perform genetic manipulation of mycobacteria is important for characterization of gene function. Homologous recombination-based protocols are frequently used for reverse genetics studies with mycobacteria. It is known that Mycobacterium bovis BCG Russia, closely related to M. bovis BCG Moreau, is a natural recA deficient strain and is non-permissive to homologous recombination assays. In this work we show that M. bovis BCG Moreau is also deficient in homologous recombination, shown by a specialized transduction assay, but this phenotype can be reverted by complementation with heterologous recombinases, using a recombineering protocol. Sequence analysis of the genes known to be involved in homologous recombination annotated in the genome of BCG Moreau detected no differences compared to the genome of BCG Pasteur. Further studies are needed in order to determine the exact mechanism underlying this deficiency in BCG Moreau.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43189/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcos Gustavo Araujo Schwarz_etal_IOC_2020.pdfMarcos Gustavo Araujo Schwarz_etal_IOC_2020.pdfapplication/pdf422638https://www.arca.fiocruz.br/bitstream/icict/43189/2/Marcos%20Gustavo%20Araujo%20Schwarz_etal_IOC_2020.pdf445fbd341b2e8f2309017492d50953ecMD52TEXTMarcos Gustavo Araujo Schwarz_etal_IOC_2020.pdf.txtMarcos Gustavo Araujo Schwarz_etal_IOC_2020.pdf.txtExtracted texttext/plain22985https://www.arca.fiocruz.br/bitstream/icict/43189/3/Marcos%20Gustavo%20Araujo%20Schwarz_etal_IOC_2020.pdf.txt74954194b60845041b9b01195b5dce49MD53icict/431892020-09-05 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dc.title.pt_BR.fl_str_mv Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
title Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
spellingShingle Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
Schwarz, Marcos Gustavo Araujo
Recombinação homóloga
Mycobacterium bovis BCG Moreau
Vacina contra tuberculose
Homologous recombination
Mycobacterium bovis BCG Moreau
Recombineering
Tuberculosis vaccine
title_short Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
title_full Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
title_fullStr Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
title_full_unstemmed Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
title_sort Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination
author Schwarz, Marcos Gustavo Araujo
author_facet Schwarz, Marcos Gustavo Araujo
Corrêa, Paloma Rezende
Malaga, Wladimir
Guilhot, Christophe
Lima, Leila Mondonça
author_role author
author2 Corrêa, Paloma Rezende
Malaga, Wladimir
Guilhot, Christophe
Lima, Leila Mondonça
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Schwarz, Marcos Gustavo Araujo
Corrêa, Paloma Rezende
Malaga, Wladimir
Guilhot, Christophe
Lima, Leila Mondonça
dc.subject.other.pt_BR.fl_str_mv Recombinação homóloga
Mycobacterium bovis BCG Moreau
Vacina contra tuberculose
topic Recombinação homóloga
Mycobacterium bovis BCG Moreau
Vacina contra tuberculose
Homologous recombination
Mycobacterium bovis BCG Moreau
Recombineering
Tuberculosis vaccine
dc.subject.en.pt_BR.fl_str_mv Homologous recombination
Mycobacterium bovis BCG Moreau
Recombineering
Tuberculosis vaccine
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-09-04T19:20:40Z
dc.date.available.fl_str_mv 2020-09-04T19:20:40Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination. Tuberculosis. v. 123, p. 1-4, June 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43189
dc.identifier.issn.pt_BR.fl_str_mv 1472-9792
dc.identifier.doi.none.fl_str_mv 10.1016/j.tube.2020.101956
identifier_str_mv SCHWARZ, Marcos Gustavo Araujo et al. Mycobacterium bovis BCG moreau is naturally deficient in homologous recombination. Tuberculosis. v. 123, p. 1-4, June 2020.
1472-9792
10.1016/j.tube.2020.101956
url https://www.arca.fiocruz.br/handle/icict/43189
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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