Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16728 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil. |
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Castro, Márcia Gonçalves deNogueira, Rita Maria RibeiroFillipis, Ana Maria Bispo deFerreira, Anielly AlvesLima, Monique da Rocha QueirozFaria, Nieli Rodrigues da CostaNogueira, Fernanda de BruyckerSimões, Jaqueline Bastos SantosNunes, Priscila Conrado GuerraSampaio, Simone AlvesOliveira, Ricardo Lourenço deSantos, Flávia Barreto dos2017-01-05T14:02:49Z2017-01-05T14:02:49Z2012CASTRO, Márcia Gonçalves de; et al. Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance. Mem Inst Oswaldo Cruz, v.107, n.7, p.940-945, Nov. 2012.0074-0276https://www.arca.fiocruz.br/handle/icict/167281678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzAedes aegyptiVírus da dengue tipo 4Reação em Cadeia da PolimeraseNiterói, RJdengue virus type 4Aedes aegyptiSimplexa™ Dengue real-time RT-PCRreal-time RT-PCRNiterói, RJDengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ. Brasil.In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and Simplexa™ Dengue real-time RT-PCR confirmed DENV-4 infection in all cases. Additionally, DENV-4 was confirmed in a female Ae. aegypti with 1.08 x 103 copies/mL of virus, as determined by quantitative real-time RT-PCR. This is the first time the Simplexa™ Dengue real-time assay has been used for the classification of cases of infection and for entomological investigations. The use of these molecular techniques was shown to be important for the surveillance of dengue in humans and vectors.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16728/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmonique_lima_etal_IOC_2012.pdfmonique_lima_etal_IOC_2012.pdfapplication/pdf678088https://www.arca.fiocruz.br/bitstream/icict/16728/2/monique_lima_etal_IOC_2012.pdfda85ecde7a4ea573bd040d529f69fed9MD52TEXTmonique_lima_etal_IOC_2012.pdf.txtmonique_lima_etal_IOC_2012.pdf.txtExtracted texttext/plain31379https://www.arca.fiocruz.br/bitstream/icict/16728/3/monique_lima_etal_IOC_2012.pdf.txtd37181b1cb78a97738fae49481ef025fMD53icict/167282018-08-15 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Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| title |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| spellingShingle |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance Castro, Márcia Gonçalves de Aedes aegypti Vírus da dengue tipo 4 Reação em Cadeia da Polimerase Niterói, RJ dengue virus type 4 Aedes aegypti Simplexa™ Dengue real-time RT-PCR real-time RT-PCR Niterói, RJ |
| title_short |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| title_full |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| title_fullStr |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| title_full_unstemmed |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| title_sort |
Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance |
| author |
Castro, Márcia Gonçalves de |
| author_facet |
Castro, Márcia Gonçalves de Nogueira, Rita Maria Ribeiro Fillipis, Ana Maria Bispo de Ferreira, Anielly Alves Lima, Monique da Rocha Queiroz Faria, Nieli Rodrigues da Costa Nogueira, Fernanda de Bruycker Simões, Jaqueline Bastos Santos Nunes, Priscila Conrado Guerra Sampaio, Simone Alves Oliveira, Ricardo Lourenço de Santos, Flávia Barreto dos |
| author_role |
author |
| author2 |
Nogueira, Rita Maria Ribeiro Fillipis, Ana Maria Bispo de Ferreira, Anielly Alves Lima, Monique da Rocha Queiroz Faria, Nieli Rodrigues da Costa Nogueira, Fernanda de Bruycker Simões, Jaqueline Bastos Santos Nunes, Priscila Conrado Guerra Sampaio, Simone Alves Oliveira, Ricardo Lourenço de Santos, Flávia Barreto dos |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Castro, Márcia Gonçalves de Nogueira, Rita Maria Ribeiro Fillipis, Ana Maria Bispo de Ferreira, Anielly Alves Lima, Monique da Rocha Queiroz Faria, Nieli Rodrigues da Costa Nogueira, Fernanda de Bruycker Simões, Jaqueline Bastos Santos Nunes, Priscila Conrado Guerra Sampaio, Simone Alves Oliveira, Ricardo Lourenço de Santos, Flávia Barreto dos |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes aegypti Vírus da dengue tipo 4 Reação em Cadeia da Polimerase Niterói, RJ |
| topic |
Aedes aegypti Vírus da dengue tipo 4 Reação em Cadeia da Polimerase Niterói, RJ dengue virus type 4 Aedes aegypti Simplexa™ Dengue real-time RT-PCR real-time RT-PCR Niterói, RJ |
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dengue virus type 4 Aedes aegypti Simplexa™ Dengue real-time RT-PCR real-time RT-PCR Niterói, RJ |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ. Brasil. |
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2012 |
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2012 |
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2017-01-05T14:02:49Z |
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2017-01-05T14:02:49Z |
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info:eu-repo/semantics/article |
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CASTRO, Márcia Gonçalves de; et al. Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance. Mem Inst Oswaldo Cruz, v.107, n.7, p.940-945, Nov. 2012. |
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https://www.arca.fiocruz.br/handle/icict/16728 |
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1678-8060 |
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CASTRO, Márcia Gonçalves de; et al. Dengue virus type 4 in Niterói, Rio de Janeiro: the role of molecular techniques in laboratory diagnosis and entomological surveillance. Mem Inst Oswaldo Cruz, v.107, n.7, p.940-945, Nov. 2012. 0074-0276 1678-8060 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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