Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17993 |
Resumo: | Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. |
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Amaral, Kátia B.Silva, Thiago P.Malta, Kássia K.Carmo, Lívia A. S.Dias, Felipe F.Almeida, Mariana E.Andrade, Gustavo F. S.Martins, Jefferson S.Pinho, Roberto R.Costa Neto, Sócrates F.Gentile, RosanaMelo, Rossana C. N.2017-03-09T16:39:25Z2017-03-09T16:39:25Z2016AMARAL, Kátia B. et al. Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment. Plos One, v.11, n.11, e0166979, 21p, Nov. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1799310.1371/journal.pone.0166979engPublic Library of ScienceSchistosoma mansoniHepatócitosInfecçãoGotículas LipídicasRato d`águaSchistosoma mansoniInfectionWild Reservoir Nectomys squamipeslipid dropletshepatocytesNatural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo Estrutura. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Química. Laboratório de Nanoestruturas Plasmônicas, Espectroscopia Molecular e Grupo Estrutura. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Física; Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Departamento de Física; Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ. Brasil.Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil.Schistosomiasis is a neglected tropical disease of a significant public health impact. The water rat Nectomys squamipes is one of the most important non-human hosts in the schistosomiasis mansoni transmission in Brazil, being considered a wild reservoir. Cellular mechanisms that contribute to the physiological adaptation of this rodent to the Schistosoma mansoni parasite are poorly understood. Here we identified, for the first time, that a hepatic steatosis, a condition characterized by excessive lipid accumulation with formation of lipid droplets (LDs) within hepatocytes, occurs in response to the natural S. mansoni infection of N. squamipes, captured in an endemic region. Significant increases of LD area in the hepatic tissue and LD numbers/hepatocyte, detected by quantitative histopathological and ultrastructural analyses, were paralleled by increased serum profile (total cholesterol and triglycerides) in infected compared to uninfected animals. Raman spectroscopy showed high content of polyunsaturated fatty acids (PUFAs) in the liver of both groups. MALDI-TOFF mass spectroscopy revealed an amplified pool of omega-6 PUFA arachidonic acid in the liver of infected animals. Assessment of liver functional activity by the levels of hepatic transaminases (ALT and AST) did not detect any alteration during the natural infection. In summary, this work demonstrates that the natural infection of the wild reservoir N. squamipes with S. mansoni elicits hepatic steatosis in the absence of liver functional harm and that accumulation of lipids, markedly PUFAs, coexists with low occurrence of inflammatory granulomatous processes, suggesting that lipid stores may be acting as a protective mechanism for dealing with the infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| title |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| spellingShingle |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment Amaral, Kátia B. Schistosoma mansoni Hepatócitos Infecção Gotículas Lipídicas Rato d`água Schistosoma mansoni Infection Wild Reservoir Nectomys squamipes lipid droplets hepatocytes |
| title_short |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| title_full |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| title_fullStr |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| title_full_unstemmed |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| title_sort |
Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment |
| author |
Amaral, Kátia B. |
| author_facet |
Amaral, Kátia B. Silva, Thiago P. Malta, Kássia K. Carmo, Lívia A. S. Dias, Felipe F. Almeida, Mariana E. Andrade, Gustavo F. S. Martins, Jefferson S. Pinho, Roberto R. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
| author_role |
author |
| author2 |
Silva, Thiago P. Malta, Kássia K. Carmo, Lívia A. S. Dias, Felipe F. Almeida, Mariana E. Andrade, Gustavo F. S. Martins, Jefferson S. Pinho, Roberto R. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
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author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Amaral, Kátia B. Silva, Thiago P. Malta, Kássia K. Carmo, Lívia A. S. Dias, Felipe F. Almeida, Mariana E. Andrade, Gustavo F. S. Martins, Jefferson S. Pinho, Roberto R. Costa Neto, Sócrates F. Gentile, Rosana Melo, Rossana C. N. |
| dc.subject.other.pt_BR.fl_str_mv |
Schistosoma mansoni Hepatócitos Infecção Gotículas Lipídicas Rato d`água |
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Schistosoma mansoni Hepatócitos Infecção Gotículas Lipídicas Rato d`água Schistosoma mansoni Infection Wild Reservoir Nectomys squamipes lipid droplets hepatocytes |
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Schistosoma mansoni Infection Wild Reservoir Nectomys squamipes lipid droplets hepatocytes |
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Universidade Federal de Juiz de Fora. Departamento de Biologia. Laboratório de Biologia Celular. Juiz de Fora, MG, Brasil. |
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2016 |
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2016 |
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2017-03-09T16:39:25Z |
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2017-03-09T16:39:25Z |
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AMARAL, Kátia B. et al. Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment. Plos One, v.11, n.11, e0166979, 21p, Nov. 2016. |
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https://www.arca.fiocruz.br/handle/icict/17993 |
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1932-6203 |
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10.1371/journal.pone.0166979 |
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AMARAL, Kátia B. et al. Natural Schistosoma mansoni Infection in the Wild Reservoir Nectomys squamipes Leads to Excessive Lipid Droplet Accumulation in Hepatocytes in the Absence of Liver Functional Impairment. Plos One, v.11, n.11, e0166979, 21p, Nov. 2016. 1932-6203 10.1371/journal.pone.0166979 |
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