Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4

Detalhes bibliográficos
Autor(a) principal: Vieira, Tamara da Silva
Data de Publicação: 2019
Outros Autores: Rugani, Jeronimo Marteleto Nunes, Nogueira, Paula Monalisa, Torrecilhas, Ana Cláudia, Gontijo, Celia Maria Ferreira, Descoteaux, Albert, Soares, Rodrigo Pedro Pinto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35059
Resumo: 2021-01-01
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spelling Vieira, Tamara da SilvaRugani, Jeronimo Marteleto NunesNogueira, Paula MonalisaTorrecilhas, Ana CláudiaGontijo, Celia Maria FerreiraDescoteaux, AlbertSoares, Rodrigo Pedro Pinto2019-08-26T13:07:42Z2019-08-26T13:07:42Z2019VIEIRA, Tamara da Silva et al. Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-9, 2019.2235-2988https://www.arca.fiocruz.br/handle/icict/3505910.3389/fcimb.2019.00240engFrontiers Media SAIntraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de São Paulo. Diadema, SP, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.INRS-Institut Armand-Frappier. Université du Québec. Laval, QC, Canada.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Lipophosphoglycan (LPG) is the major Leishmania surface glycoconjugate having importance during the host-parasite interface. Leishmania (Viannia) braziliensis displays a spectrum of clinical forms including: typical cutaneous leishmaniasis (TL), mucocutaneous (ML), and atypical lesions (AL). Those variations in the immunopathology may be a result of intraspecies polymorphisms in the parasite's virulence factors. In this context, we evaluated the role of LPG of strains originated from patients with different clinical manifestations and the sandfly vector. Six isolates of L. braziliensis were used: M2903, RR051 and RR418 (TL), RR410 (AL), M15991 (ML), and M8401 (vector). LPGs were extracted and purified by hydrophobic interaction. Peritoneal macrophages from C57BL/6 and respective knock-outs (TLR2-/- and TLR-4-/-) were primed with IFN-γ and exposed to different LPGs for nitric oxide (NO) and cytokine production (IL-1β, IL-6, IL-12, and TNF-α). LPGs differentially activated the production of NO and cytokines via TLR4. In order to ascertain if such functional variations were related to intraspecies polymorphisms in the LPG, the purified glycoconjugates were subjected to western blot with specific LPG antibodies (CA7AE and LT22). Based on antibody reactivity preliminary variations in the repeat units were detected. To confirm these findings, LPGs were depolymerized for purification of repeat units. After thin layer chromatography, intraspecies polymorphisms were confirmed especially in the type and/size of sugars branching-off the repeat units motif. In conclusion, different isolates of L. braziliensis from different clinical forms and hosts possess polymorphisms in their LPGs that functionally affected macrophage responses.Leishmania braziliensisClinical formsInnate immunityLipophosphoglycanMacrophagesVirulenceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/35059/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALIntraspecies Polymorphisms.pdfIntraspecies Polymorphisms.pdfapplication/pdf2088740https://www.arca.fiocruz.br/bitstream/icict/35059/2/Intraspecies%20Polymorphisms.pdf9a4e0f40c71e17f1f4caf8f233828a16MD52TEXTIntraspecies Polymorphisms.pdf.txtIntraspecies Polymorphisms.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
title Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
spellingShingle Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
Vieira, Tamara da Silva
Leishmania braziliensis
Clinical forms
Innate immunity
Lipophosphoglycan
Macrophages
Virulence
title_short Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
title_full Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
title_fullStr Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
title_full_unstemmed Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
title_sort Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4
author Vieira, Tamara da Silva
author_facet Vieira, Tamara da Silva
Rugani, Jeronimo Marteleto Nunes
Nogueira, Paula Monalisa
Torrecilhas, Ana Cláudia
Gontijo, Celia Maria Ferreira
Descoteaux, Albert
Soares, Rodrigo Pedro Pinto
author_role author
author2 Rugani, Jeronimo Marteleto Nunes
Nogueira, Paula Monalisa
Torrecilhas, Ana Cláudia
Gontijo, Celia Maria Ferreira
Descoteaux, Albert
Soares, Rodrigo Pedro Pinto
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Tamara da Silva
Rugani, Jeronimo Marteleto Nunes
Nogueira, Paula Monalisa
Torrecilhas, Ana Cláudia
Gontijo, Celia Maria Ferreira
Descoteaux, Albert
Soares, Rodrigo Pedro Pinto
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Clinical forms
Innate immunity
Lipophosphoglycan
Macrophages
Virulence
topic Leishmania braziliensis
Clinical forms
Innate immunity
Lipophosphoglycan
Macrophages
Virulence
description 2021-01-01
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-26T13:07:42Z
dc.date.available.fl_str_mv 2019-08-26T13:07:42Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIEIRA, Tamara da Silva et al. Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35059
dc.identifier.issn.pt_BR.fl_str_mv 2235-2988
dc.identifier.doi.none.fl_str_mv 10.3389/fcimb.2019.00240
identifier_str_mv VIEIRA, Tamara da Silva et al. Intraspecies Polymorphisms in the Lipophosphoglycan of L. braziliensis Differentially Modulate Macrophage Activation via TLR4. Frontiers in Cellular and Infection Microbiology, v. 9, p. 1-9, 2019.
2235-2988
10.3389/fcimb.2019.00240
url https://www.arca.fiocruz.br/handle/icict/35059
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35059/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/35059/2/Intraspecies%20Polymorphisms.pdf
https://www.arca.fiocruz.br/bitstream/icict/35059/3/Intraspecies%20Polymorphisms.pdf.txt
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