Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32455 |
Resumo: | This research project was funded by a Gonçalo Moniz Institute, Oswaldo Cruz Foundation (Fiocruz/BA). |
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Dopico, EvaDel-Rei, Rodrigo PimentaEspinoza, BerthaUbillos, ItziarZanchin, Nilson Ivo ToninSulleiro, ElenaMoure, ZairaCeledon, Paola Alejandra FioraniSouza, Wayner VieiraSilva, Edimilson Domingos daGomes, Yara MirandaSantos, Fred Luciano Neves2019-04-11T18:23:25Z2019-04-11T18:23:25Z2019DOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/3245510.1186/s12879-019-3872-zThis research project was funded by a Gonçalo Moniz Institute, Oswaldo Cruz Foundation (Fiocruz/BA).Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain.Faculty of Technology and Sciences of Bahia. Salvador, Bahia, Brazil.Universidad Nacional Autónoma de México. Instituto de Investigaciones Biomédicas. Departamento de Inmunología. Ciudad de México, Mexico.Catalan Institute of Health. Laboratori Clínic Territorial Metropolitana Sud. Barcelona, Catalonia, Spain.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain.Vall d’Hebron University Hospital. Microbiology Department.Barcelona, Catalonia, Spain.Institute of Paraná. Molecular Biology. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Chronic Chagas Disease (CD) diagnosis is based on serological methods employing crude, semipurified or recombinant antigens, which may result in low sensitivity or cross-reactivity. To reduce these restrictions, we developed a strategy involving use of molecules containing repetitive fragments of Trypanosoma cruzi conserved proteins. Diagnostic performance of IBMP-8.1 and IBMP-8.4 chimeric antigens (Molecular Biology Institute of Paraná - IBMP in Portuguese acronym) was assessed to diagnose T. cruzi-infected and non-infected immigrants living in Barcelona (Spain), a non-endemic setting for Chagas disease. Methods: Reactivity of IBMP-8.1 and IBMP-8.4 was assessed using an in-house automated ELISA with 347 positive and 331 negative individuals to Chagas disease. Antigenic cross-reactivity was measured with sera samples from pregnant women with Toxoplasma gondii (n = 98) and Zika virus (n = 75) antibodies. Results: The area under the curve values was 1 and 0.99 for the IBMP-8.1 and IBMP-8.4 proteins, respectively, demonstrating excellent diagnostic accuracy. The reactivity index was higher for IBMP-8.1 than IBMP-8.4 in positive samples and no significant difference in reactivity index was observed in negative samples. Sensitivity ranged from 99.4% for IBMP-8.1 to 99.1% for IBMP-8.4 and was not statistically different. Specificity for IBMP-8.1 reached 100 and 99.7% for IBMP-8.4, both nearly 100% accurate. No antigenic cross-reactivity was observed and reactivity index was similar to that for negative Chagas disease individuals. Conclusions: Our results showed an outstanding performance of IBMP-8.1 and IBMP-8.4 chimeric antigens by ELISA and suggest both chimeric antigens could also be used for Chagas disease diagnosis in immigrants living in nonendemic settings.engBMCDoença de ChagasTrypanosoma cruziAntígenos QuiméricosImunoensaioPrecisãoChagas diseaseTrypanosoma cruziChimeric antigensImmunoassayAccuracyAdultoAnticorpos AntiprotozoáriosImunologiaAntígenos de ProtozoáriosGenéticaDoença de ChagasDiagnósticoParasitologiaReações CruzadasEmigrantes e ImigrantesEstatística & dados numéricosEnsaio de Imunoadsorção EnzimáticaFemininoHumanosMasculinoGravidezSensibilidade e EspecificidadeEspanhaToxoplasmaTrypanosoma cruziImmune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic settinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| title |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| spellingShingle |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting Dopico, Eva Doença de Chagas Trypanosoma cruzi Antígenos Quiméricos Imunoensaio Precisão Chagas disease Trypanosoma cruzi Chimeric antigens Immunoassay Accuracy Adulto Anticorpos Antiprotozoários Imunologia Antígenos de Protozoários Genética Doença de Chagas Diagnóstico Parasitologia Reações Cruzadas Emigrantes e Imigrantes Estatística & dados numéricos Ensaio de Imunoadsorção Enzimática Feminino Humanos Masculino Gravidez Sensibilidade e Especificidade Espanha Toxoplasma Trypanosoma cruzi |
| title_short |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| title_full |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| title_fullStr |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| title_full_unstemmed |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| title_sort |
Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting |
| author |
Dopico, Eva |
| author_facet |
Dopico, Eva Del-Rei, Rodrigo Pimenta Espinoza, Bertha Ubillos, Itziar Zanchin, Nilson Ivo Tonin Sulleiro, Elena Moure, Zaira Celedon, Paola Alejandra Fiorani Souza, Wayner Vieira Silva, Edimilson Domingos da Gomes, Yara Miranda Santos, Fred Luciano Neves |
| author_role |
author |
| author2 |
Del-Rei, Rodrigo Pimenta Espinoza, Bertha Ubillos, Itziar Zanchin, Nilson Ivo Tonin Sulleiro, Elena Moure, Zaira Celedon, Paola Alejandra Fiorani Souza, Wayner Vieira Silva, Edimilson Domingos da Gomes, Yara Miranda Santos, Fred Luciano Neves |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Dopico, Eva Del-Rei, Rodrigo Pimenta Espinoza, Bertha Ubillos, Itziar Zanchin, Nilson Ivo Tonin Sulleiro, Elena Moure, Zaira Celedon, Paola Alejandra Fiorani Souza, Wayner Vieira Silva, Edimilson Domingos da Gomes, Yara Miranda Santos, Fred Luciano Neves |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de Chagas Trypanosoma cruzi Antígenos Quiméricos Imunoensaio Precisão |
| topic |
Doença de Chagas Trypanosoma cruzi Antígenos Quiméricos Imunoensaio Precisão Chagas disease Trypanosoma cruzi Chimeric antigens Immunoassay Accuracy Adulto Anticorpos Antiprotozoários Imunologia Antígenos de Protozoários Genética Doença de Chagas Diagnóstico Parasitologia Reações Cruzadas Emigrantes e Imigrantes Estatística & dados numéricos Ensaio de Imunoadsorção Enzimática Feminino Humanos Masculino Gravidez Sensibilidade e Especificidade Espanha Toxoplasma Trypanosoma cruzi |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease Trypanosoma cruzi Chimeric antigens Immunoassay Accuracy |
| dc.subject.decs.pt_BR.fl_str_mv |
Adulto Anticorpos Antiprotozoários Imunologia Antígenos de Protozoários Genética Doença de Chagas Diagnóstico Parasitologia Reações Cruzadas Emigrantes e Imigrantes Estatística & dados numéricos Ensaio de Imunoadsorção Enzimática Feminino Humanos Masculino Gravidez Sensibilidade e Especificidade Espanha Toxoplasma Trypanosoma cruzi |
| description |
This research project was funded by a Gonçalo Moniz Institute, Oswaldo Cruz Foundation (Fiocruz/BA). |
| publishDate |
2019 |
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2019-04-11T18:23:25Z |
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2019-04-11T18:23:25Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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| dc.identifier.citation.fl_str_mv |
DOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019. |
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https://www.arca.fiocruz.br/handle/icict/32455 |
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1471-2334 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s12879-019-3872-z |
| identifier_str_mv |
DOPICO, Eva et al. Immune reactivity to Trypanosoma cruzi chimeric proteins for Chagas disease diagnosis in immigrants living in a non-endemic setting. BMC Infectious Diseases, v. 19, p. 1-7, 2019. 1471-2334 10.1186/s12879-019-3872-z |
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BMC |
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