IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin

Detalhes bibliográficos
Autor(a) principal: De Simone, Salvatore Giovanni
Data de Publicação: 2014
Outros Autores: Pêgo, Paloma Napoleão, Pinto, Luiz A. L. Teixeira, Melgarejo, Anibal R., Aguiar, Aniesse S., Provance Jr., David W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10932
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
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spelling De Simone, Salvatore GiovanniPêgo, Paloma NapoleãoPinto, Luiz A. L. TeixeiraMelgarejo, Anibal R.Aguiar, Aniesse S.Provance Jr., David W.2015-06-22T12:26:36Z2015-06-22T12:26:36Z2014DE SIMONE, Salvatore Giovanni et al. IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin. Toxicon, v. 78, p. 83-93, 2014.0041-0101https://www.arca.fiocruz.br/handle/icict/1093210.1016/j.toxicon.2013.12.001engElsevierIgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Instituto Vital Brasil. Laboratório de Pesquisa e Desenvolvimento. Niterói, RJ, Brasil.Instituto Vital Brasil. Laboratório de Pesquisa e Desenvolvimento. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil.The presence of whole horse IgG in therapeutic snake antivenom preparations of high purity is a contamination that can cause IgE-mediated allergic reactions in patients. In this study, the immunodominant IgE and IgG-binding epitopes in horse heavy chain IgG3 were mapped using arrays of overlapping peptides synthesized directly onto activated cellulose membranes. Pooled human sera from patients with and without horse antivenom allergies were used to probe the membrane. We have demonstrated that, for both cases, individuals produce antibodies to epitopes of sequential amino acids of horse heavy chain IgG3, although the signal strength and specificity appear to be distinct between the two groups of patients. A single region was found to contain the dominant allergic IgE epitope. The critical residues involved in the binding of human IgE to the epitope were determined to include four hydrophobic amino acids followed by polar and charged residues that formed a coil structure. This is the first study to describe the specific amino acid sequences involved with the immune recognition of human IgG and IgE to horse antivenom.Horse immunoglobulinB-linear epitopesIgE epitopeIgG epitopeAnaphylactic reactionSpot-synthesisAnafilaxiaEpitoposinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10932/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALpaloma-pegoetal_ioc_2014.pdfapplication/pdf2267098https://www.arca.fiocruz.br/bitstream/icict/10932/2/paloma-pegoetal_ioc_2014.pdf152ce68692a54d462431ecd2cc9d46a5MD52TEXTpaloma-pegoetal_ioc_2014.pdf.txtpaloma-pegoetal_ioc_2014.pdf.txtExtracted texttext/plain48348https://www.arca.fiocruz.br/bitstream/icict/10932/3/paloma-pegoetal_ioc_2014.pdf.txt26309a65f6e7aeb8fe28aeabc53cc433MD53icict/109322023-07-28 13:47:33.136oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-28T16:47:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
title IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
spellingShingle IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
De Simone, Salvatore Giovanni
Horse immunoglobulin
B-linear epitopes
IgE epitope
IgG epitope
Anaphylactic reaction
Spot-synthesis
Anafilaxia
Epitopos
title_short IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
title_full IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
title_fullStr IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
title_full_unstemmed IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
title_sort IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin
author De Simone, Salvatore Giovanni
author_facet De Simone, Salvatore Giovanni
Pêgo, Paloma Napoleão
Pinto, Luiz A. L. Teixeira
Melgarejo, Anibal R.
Aguiar, Aniesse S.
Provance Jr., David W.
author_role author
author2 Pêgo, Paloma Napoleão
Pinto, Luiz A. L. Teixeira
Melgarejo, Anibal R.
Aguiar, Aniesse S.
Provance Jr., David W.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv De Simone, Salvatore Giovanni
Pêgo, Paloma Napoleão
Pinto, Luiz A. L. Teixeira
Melgarejo, Anibal R.
Aguiar, Aniesse S.
Provance Jr., David W.
dc.subject.en.pt_BR.fl_str_mv Horse immunoglobulin
B-linear epitopes
IgE epitope
IgG epitope
Anaphylactic reaction
Spot-synthesis
topic Horse immunoglobulin
B-linear epitopes
IgE epitope
IgG epitope
Anaphylactic reaction
Spot-synthesis
Anafilaxia
Epitopos
dc.subject.decs.pt_BR.fl_str_mv Anafilaxia
Epitopos
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-22T12:26:36Z
dc.date.available.fl_str_mv 2015-06-22T12:26:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DE SIMONE, Salvatore Giovanni et al. IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin. Toxicon, v. 78, p. 83-93, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10932
dc.identifier.issn.pt_BR.fl_str_mv 0041-0101
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.toxicon.2013.12.001
identifier_str_mv DE SIMONE, Salvatore Giovanni et al. IgE and IgG epitope mapping by microarray peptideimmunoassay reveals the importance and diversity of the immune response to the IgG3 equine immunoglobulin. Toxicon, v. 78, p. 83-93, 2014.
0041-0101
10.1016/j.toxicon.2013.12.001
url https://www.arca.fiocruz.br/handle/icict/10932
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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