Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations

Detalhes bibliográficos
Autor(a) principal: De Simone, Salvatore Giovanni
Data de Publicação: 2013
Outros Autores: Gomes, Luciano Pinto, Gemal, André Luis, Silva, Filipe Soares Quirino da, Provence Junior, David William
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8634
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento de Tecnologias da Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. / Universidade Federal Fluminense. Departamento de Biologia Molecular e Celular. Niterói, RJ, Brasil.
id CRUZ_cdef7ce5da8a8f7607b3c44eb660f8aa
oai_identifier_str oai:www.arca.fiocruz.br:icict/8634
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling De Simone, Salvatore GiovanniGomes, Luciano PintoGemal, André LuisSilva, Filipe Soares Quirino daProvence Junior, David William2014-10-21T13:07:23Z2014-10-21T13:07:23Z2013DE SIMONE, S. G. et al. Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations. Journal of Biotechnology Letters, v. 4, n. 2, p. 84-90. 2013.0976-7045https://www.arca.fiocruz.br/handle/icict/86340976-7053engBioinfo PublicationsDetermination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento de Tecnologias da Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. / Universidade Federal Fluminense. Departamento de Biologia Molecular e Celular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Química. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento de Tecnologias da Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil.Equine immunoglobulins (eqIg) andits fragments, F(ab’)2and F(ab’), are normally employed as immunopharmaceuticals for several diseases, like treatment of snake bits and neutralizing bacterial toxins. However, it can induce serious side effects in a subset of patients, spe-cifically hypersensitivity from their immune response against whole eqIg contaminants. Therefore, the important antigenic determinants in eq-uine heavy chain IgG3(eqIgG3) were defined by a library of overlapping 15-mer peptides that covered the entire amino acid sequence (354 residues) and were immobilized onto a membrane.Screening with rabbit sera identified eleven distinct epitopes in eqIgG3 that were recog-nized by rabbit antibodies. The performance of an antisera against the most prominent epitope was analyzed by ELISA and a competitive assay. The results suggest an effective antisera to detect whole, undigested eqIg within equine sera preparations for minimizing adverse side effects and support this methodology for producing targeted antibodies.Equine ImmunoglobulinPeptide ArraySPOT-synthesisB-epitopesSynthetic peptidesImunoglobulinasPeptídeosTécnicas de Síntese em Fase SólidaEpitopos de Linfócito BPeptídeo Sintasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8634/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALJ Biotechnol Lett_4_84-90.pdfJ Biotechnol Lett_4_84-90.pdfapplication/pdf953261https://www.arca.fiocruz.br/bitstream/icict/8634/2/J%20Biotechnol%20Lett_4_84-90.pdfd164de5474296f1f3e3b832e8f0f6d56MD52TEXTJ Biotechnol Lett_4_84-90.pdf.txtJ Biotechnol Lett_4_84-90.pdf.txtExtracted texttext/plain38197https://www.arca.fiocruz.br/bitstream/icict/8634/3/J%20Biotechnol%20Lett_4_84-90.pdf.txt040bbe1bfd139add6c41d01d7a849114MD53icict/86342018-04-06 08:43:54.6oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en.fl_str_mv Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
title Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
spellingShingle Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
De Simone, Salvatore Giovanni
Equine Immunoglobulin
Peptide Array
SPOT-synthesis
B-epitopes
Synthetic peptides
Imunoglobulinas
Peptídeos
Técnicas de Síntese em Fase Sólida
Epitopos de Linfócito B
Peptídeo Sintases
title_short Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
title_full Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
title_fullStr Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
title_full_unstemmed Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
title_sort Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations
author De Simone, Salvatore Giovanni
author_facet De Simone, Salvatore Giovanni
Gomes, Luciano Pinto
Gemal, André Luis
Silva, Filipe Soares Quirino da
Provence Junior, David William
author_role author
author2 Gomes, Luciano Pinto
Gemal, André Luis
Silva, Filipe Soares Quirino da
Provence Junior, David William
author2_role author
author
author
author
dc.contributor.author.fl_str_mv De Simone, Salvatore Giovanni
Gomes, Luciano Pinto
Gemal, André Luis
Silva, Filipe Soares Quirino da
Provence Junior, David William
dc.subject.en.en.fl_str_mv Equine Immunoglobulin
Peptide Array
SPOT-synthesis
B-epitopes
Synthetic peptides
topic Equine Immunoglobulin
Peptide Array
SPOT-synthesis
B-epitopes
Synthetic peptides
Imunoglobulinas
Peptídeos
Técnicas de Síntese em Fase Sólida
Epitopos de Linfócito B
Peptídeo Sintases
dc.subject.decs.pt_BR.fl_str_mv Imunoglobulinas
Peptídeos
Técnicas de Síntese em Fase Sólida
Epitopos de Linfócito B
Peptídeo Sintases
description Fundação Oswaldo Cruz. Centro de Desenvolvimento de Tecnologias da Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. / Universidade Federal Fluminense. Departamento de Biologia Molecular e Celular. Niterói, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-21T13:07:23Z
dc.date.available.fl_str_mv 2014-10-21T13:07:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DE SIMONE, S. G. et al. Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations. Journal of Biotechnology Letters, v. 4, n. 2, p. 84-90. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8634
dc.identifier.issn.none.fl_str_mv 0976-7045
dc.identifier.eissn.none.fl_str_mv 0976-7053
identifier_str_mv DE SIMONE, S. G. et al. Determination of a linear B-cell epitope in equine IgG3 antibodies for improved detection in therapeutic preparations. Journal of Biotechnology Letters, v. 4, n. 2, p. 84-90. 2013.
0976-7045
0976-7053
url https://www.arca.fiocruz.br/handle/icict/8634
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bioinfo Publications
publisher.none.fl_str_mv Bioinfo Publications
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8634/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/8634/2/J%20Biotechnol%20Lett_4_84-90.pdf
https://www.arca.fiocruz.br/bitstream/icict/8634/3/J%20Biotechnol%20Lett_4_84-90.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
d164de5474296f1f3e3b832e8f0f6d56
040bbe1bfd139add6c41d01d7a849114
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324719552823296

Registros relacionados