Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine

Detalhes bibliográficos
Autor(a) principal: Silva, Flavio R. da
Data de Publicação: 2014
Outros Autores: Pego, Paloma Napoleão, De Simone, Salvatore Giovanni
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10171
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Flavio R. daPego, Paloma NapoleãoDe Simone, Salvatore Giovanni2015-05-04T16:34:33Z2015-05-04T16:34:33Z2014SILVA, Flavio R. da; PEGO, Paloma Napoleão; DE SIMONE, Salvatore Giovanni. Identification of linear B epitopes of pertactin of Bordetella pertussisinduced by immunization with whole and acellular vaccine. Vaccine, v. 32, n. 47, p. 6251-6258, 2014.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/1017110.1016/j.vaccine.2014.09.019engElsevierIdentification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Pertussis is a serious infectious disease of the respiratory tract caused by the gram-negative bacteria Bor-detella pertussis. There has been a reemergence of this disease within the population of several countriesthat have well established vaccination programs. Analyzes of clinical isolates suggest an antigenic diver-gence between the vaccine-based strains to the circulating strains. Although antibodies against P.69are involved in the observed protective immunity, the sequences recognized as antigenic determinantsin P.133, the precursor for P.69, P.3.4 and P.30, have not be determined. Here, the precise mapping oflinear B-cell epitopes within the predicted P.133 pertactin sequences was accomplished using the SPOT-synthesis of peptide arrays onto cellulose membranes and screening with murine sera generated byvaccination with either the Pertussis cellular (miPc) or Pertussis acellular (miPa) vaccine. A total of 23major epitopes were identified by sera from miPc vaccinated mice, while thirteen were identified bysera from miPa vaccinated mice. Of these epitopes, 12 epitopes were specifically identified by antibodiesproduced in response to the miPc vaccine and two were specific to the miPa vaccine. These epitopeswere distributed throughout the pertactin sequence but a significant number were concentrated to theP.30 Prn segment. An analysis of the epitope correlation homologies indicated that the variations fromthe observed mutations in pertactin would not constitute a problem using these vaccines. In addition,the mapping of epitopes demonstrated a higher number of linear B-cell epitopes immunized with the Pcvaccine than the Pc vaccine.Bordetella pertussisPertactinB-epitopePeptide arraySPOT-synthesisSynthetic peptidesainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALve_Flavio_Silva_etal_CDTS_IOC_2014.pdfve_Flavio_Silva_etal_CDTS_IOC_2014.pdfapplication/pdf4549341https://www.arca.fiocruz.br/bitstream/icict/10171/2/ve_Flavio_Silva_etal_CDTS_IOC_2014.pdfe8c18f137c3a67de79c07be6dad895f5MD52LICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10171/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51icict/101712023-07-28 11:41:33.932oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-28T14:41:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
title Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
spellingShingle Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
Silva, Flavio R. da
Bordetella pertussis
Pertactin
B-epitope
Peptide array
SPOT-synthesis
Synthetic peptidesa
title_short Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
title_full Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
title_fullStr Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
title_full_unstemmed Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
title_sort Identification of linear B epitopes of pertactin of Bordetella pertussis induced by immunization with whole and acellular vaccine
author Silva, Flavio R. da
author_facet Silva, Flavio R. da
Pego, Paloma Napoleão
De Simone, Salvatore Giovanni
author_role author
author2 Pego, Paloma Napoleão
De Simone, Salvatore Giovanni
author2_role author
author
dc.contributor.author.fl_str_mv Silva, Flavio R. da
Pego, Paloma Napoleão
De Simone, Salvatore Giovanni
dc.subject.en.pt_BR.fl_str_mv Bordetella pertussis
Pertactin
B-epitope
Peptide array
SPOT-synthesis
Synthetic peptidesa
topic Bordetella pertussis
Pertactin
B-epitope
Peptide array
SPOT-synthesis
Synthetic peptidesa
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia de Inovação em Doenças Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T16:34:33Z
dc.date.available.fl_str_mv 2015-05-04T16:34:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Flavio R. da; PEGO, Paloma Napoleão; DE SIMONE, Salvatore Giovanni. Identification of linear B epitopes of pertactin of Bordetella pertussisinduced by immunization with whole and acellular vaccine. Vaccine, v. 32, n. 47, p. 6251-6258, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10171
dc.identifier.issn.pt_BR.fl_str_mv 0264-410X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.vaccine.2014.09.019
identifier_str_mv SILVA, Flavio R. da; PEGO, Paloma Napoleão; DE SIMONE, Salvatore Giovanni. Identification of linear B epitopes of pertactin of Bordetella pertussisinduced by immunization with whole and acellular vaccine. Vaccine, v. 32, n. 47, p. 6251-6258, 2014.
0264-410X
10.1016/j.vaccine.2014.09.019
url https://www.arca.fiocruz.br/handle/icict/10171
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10171/2/ve_Flavio_Silva_etal_CDTS_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10171/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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