Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Siqueira Filho, Ezequias Pessoa
Data de Publicação: 2014
Outros Autores: Zani, Carlos Leomar, Alves, Tânia Maria de Almeida, Parreiras, Patrícia Martins, Martins Filho, Olindo Assis, Araújo, Márcio Sobreira Silva, Teixeira, Eliane Morais, Melo, Marília Martins, Serákides, Rogéria, Cota, Betania Barros
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9576
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.
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spelling Siqueira Filho, Ezequias PessoaZani, Carlos LeomarAlves, Tânia Maria de AlmeidaParreiras, Patrícia MartinsMartins Filho, Olindo AssisAraújo, Márcio Sobreira SilvaTeixeira, Eliane MoraisMelo, Marília MartinsSerákides, RogériaCota, Betania Barros2015-03-02T11:52:54Z2015-03-02T11:52:54Z2014SIQUEIRA FILHO, Ezequias Pessoa. Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis. J. Nat. Prod. Plant Resour, v. 4, n. 3, p. 30-38, 20142231 – 3184https://www.arca.fiocruz.br/handle/icict/9576engACS Publ.Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Departamento de Fisiologia e Biofisica. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento Clinico e Cirurgico Veterinario. Laboratorio de Patologia e Toxicologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.With the purpose of discovery leishmanicidal secondary metabolites from natural products, crude ethanolic extract (EE) from stems of the Caesalpinia echinata was assayed to verify its in vitro leishmanicidal activity. The EE showed in vitro growth inhibition activities of 90% against amastigote-like of Leishmania (Leishmania) amazonensis. The EE was then submitted to fractionation by Gel Permeation Chromatography (GPC) yielding fifteen fractions (F1 to F15). The same biological assay was performed for the fifteen fractions and the fractions F9 to F11 showed in vitro growth inhibition activities around 80%. The fractions F9 to F11 were pooled to produce an enriched fraction named EF. Evaluation of the acute toxicity of the EE and EF were carried out with Swiss-Webster mice, orally treated by a single oral dose of 300mg of the samples (EE and EF)/kg of body to verify changes in hematological and biochemical profiles and 5.0g of the samples (EE and EF)/kg body to verify the toxicity and safety in using EE and EF as therapeutic agents in the treatment of the leishmaniasis. After preliminary results, the LD50 concentration was estimated to be greater than 5.0g/kg body for both samples (EE and EF) by oral route. The EE and EF of the C. echinata were actives in vitro experiments and nontoxic for mice, moreover these experiments proved to be the first steps towards the development of leishmanicidal agents from C. echinata.Caesalpinia echinataLeishmaniasisLeishmanicidal activityAcute toxicityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9576/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_117.pdf2014_117.pdfapplication/pdf707401https://www.arca.fiocruz.br/bitstream/icict/9576/2/2014_117.pdf0f71bb7341baea2f96798e66b6aaca36MD52TEXT2014_117.pdf.txt2014_117.pdf.txtExtracted texttext/plain33685https://www.arca.fiocruz.br/bitstream/icict/9576/3/2014_117.pdf.txt1e374da1dec4266557a0f0a568c3e438MD53icict/95762019-06-19 10:07:44.968oai:www.arca.fiocruz.br:icict/9576TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
title Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
spellingShingle Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
Siqueira Filho, Ezequias Pessoa
Caesalpinia echinata
Leishmaniasis
Leishmanicidal activity
Acute toxicity
title_short Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
title_full Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
title_fullStr Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
title_full_unstemmed Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
title_sort Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis
author Siqueira Filho, Ezequias Pessoa
author_facet Siqueira Filho, Ezequias Pessoa
Zani, Carlos Leomar
Alves, Tânia Maria de Almeida
Parreiras, Patrícia Martins
Martins Filho, Olindo Assis
Araújo, Márcio Sobreira Silva
Teixeira, Eliane Morais
Melo, Marília Martins
Serákides, Rogéria
Cota, Betania Barros
author_role author
author2 Zani, Carlos Leomar
Alves, Tânia Maria de Almeida
Parreiras, Patrícia Martins
Martins Filho, Olindo Assis
Araújo, Márcio Sobreira Silva
Teixeira, Eliane Morais
Melo, Marília Martins
Serákides, Rogéria
Cota, Betania Barros
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Siqueira Filho, Ezequias Pessoa
Zani, Carlos Leomar
Alves, Tânia Maria de Almeida
Parreiras, Patrícia Martins
Martins Filho, Olindo Assis
Araújo, Márcio Sobreira Silva
Teixeira, Eliane Morais
Melo, Marília Martins
Serákides, Rogéria
Cota, Betania Barros
dc.subject.en.pt_BR.fl_str_mv Caesalpinia echinata
Leishmaniasis
Leishmanicidal activity
Acute toxicity
topic Caesalpinia echinata
Leishmaniasis
Leishmanicidal activity
Acute toxicity
description Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Quimica de Produtos Naturais. Belo Horizonte, MG, Brazil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-03-02T11:52:54Z
dc.date.available.fl_str_mv 2015-03-02T11:52:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SIQUEIRA FILHO, Ezequias Pessoa. Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis. J. Nat. Prod. Plant Resour, v. 4, n. 3, p. 30-38, 2014
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9576
dc.identifier.issn.none.fl_str_mv 2231 – 3184
identifier_str_mv SIQUEIRA FILHO, Ezequias Pessoa. Evaluation of the In vitro leishmanicidal and In vivo acute oral toxicity of the Caesalpinia echinata L. extracts as source of natural products against Leishmaniasis. J. Nat. Prod. Plant Resour, v. 4, n. 3, p. 30-38, 2014
2231 – 3184
url https://www.arca.fiocruz.br/handle/icict/9576
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ACS Publ.
publisher.none.fl_str_mv ACS Publ.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/9576/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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