Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon

Detalhes bibliográficos
Autor(a) principal: Lima Junior, J. C.
Data de Publicação: 2010
Outros Autores: Banic, D. M., Tran, T. M., Meyer, V. S. E., Simone, S. G. de, Santos, F., Porto, L. C. S., Marques, M. T. Q., Moreno, A., Barnwell, J. W., Galinski, M. R., Ferreira, J. Oliveira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/21059
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
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spelling Lima Junior, J. C.Banic, D. M.Tran, T. M.Meyer, V. S. E.Simone, S. G. deSantos, F.Porto, L. C. S.Marques, M. T. Q.Moreno, A.Barnwell, J. W.Galinski, M. R.Ferreira, J. Oliveira2017-09-19T15:14:40Z2017-09-19T15:14:40Z2010LIMA JUNIOR, J. C. et al. Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon. Vaccine, v.28, n.18, p.3185-3191, Apr. 2010.0264-410Xhttps://www.arca.fiocruz.br/handle/icict/210591873-2518engElsevierPlasmodium vivaxEpitopos de Linfócito TPlasmodium vivaxMerozoite Surface Protein-9;promiscuous T-cell epitopesPromiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ. Brasil.Emory University. Emory Vaccine Center. Atlanta, GA, USA.Emory University. Emory Vaccine Center. Atlanta, GA, USA.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.LACEN. Departamento de Entomologia. Porto Velho, RO, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Histocompatibilidade e Criopreservação. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Laboratório de Histocompatibilidade e Criopreservação. Rio de Janeiro, RJ, Brasil.Emory University. Emory Vaccine Center. Atlanta, GA, USA / Emory University Shool of Medicine. Division of Infectious Diseases. Atlanta, GA, USA.CDC/National Center for Infectious Diseases.Emory University. Emory Vaccine Center. Atlanta, GA, USA / Emory University Shool of Medicine. Division of Infectious Diseases. Atlanta, GA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Plasmodium vivax merozoite surface protein (PvMSP9) stimulates both cellular and humoral immune responses in individuals who are naturally infected by this parasite species. To identify immunodominant human T-cell epitopes in PvMSP9, we used the MHC class II binding peptide prediction algorithm ProPred. Eleven synthetic peptides representing predicted putative promiscuous T cell epitopes were tested in IFN-γ and IL-4 ELISPOT assays using peripheral blood mononuclear cells (PBMC) derived from 142 individuals from Rondonia State, Brazil who had been naturally exposed to P. vivax infections. To determine whether the predicted epitopes are preferentially recognized in the context of multiple alleles, MHC Class II typing of the cohort was also performed. Five synthetic peptides elicited robust cellular responses, and the overall frequencies of IFN-γ and IL-4 responders to at least one of the promiscuous peptides were 62% and 46%, respectively. The frequencies of IFN-γ and IL-4 responders to each peptide were not associated with a particular HLA-DRB1 allelic group since most of the peptides induced a response in individuals of 12 out of 13 studied allelic groups. The prediction of promiscuous epitopes using ProPred led to the identification of immunodominant epitopes recognized by PBMC from a significant proportion of a genetically heterogeneous population exposed to malaria infections. The combination of several such T-cell epitopes in a vaccine construct may increase the frequency of responders and the overall efficacy of subunit vaccines in genetically distinct populations.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/21059/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSG_Simone_etal_IOC_2010.pdfSG_Simone_etal_IOC_2010.pdfapplication/pdf544053https://www.arca.fiocruz.br/bitstream/icict/21059/2/SG_Simone_etal_IOC_2010.pdfa3a36e2914636cfd9a16503b94b82949MD52TEXTSG_Simone_etal_IOC_2010.pdf.txtSG_Simone_etal_IOC_2010.pdf.txtExtracted texttext/plain49567https://www.arca.fiocruz.br/bitstream/icict/21059/3/SG_Simone_etal_IOC_2010.pdf.txte48004f116e0c2601da59e5718597590MD53icict/210592018-08-15 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dc.title.pt_BR.fl_str_mv Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
title Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
spellingShingle Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
Lima Junior, J. C.
Plasmodium vivax
Epitopos de Linfócito T
Plasmodium vivax
Merozoite Surface Protein-9;
promiscuous T-cell epitopes
title_short Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
title_full Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
title_fullStr Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
title_full_unstemmed Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
title_sort Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon
author Lima Junior, J. C.
author_facet Lima Junior, J. C.
Banic, D. M.
Tran, T. M.
Meyer, V. S. E.
Simone, S. G. de
Santos, F.
Porto, L. C. S.
Marques, M. T. Q.
Moreno, A.
Barnwell, J. W.
Galinski, M. R.
Ferreira, J. Oliveira
author_role author
author2 Banic, D. M.
Tran, T. M.
Meyer, V. S. E.
Simone, S. G. de
Santos, F.
Porto, L. C. S.
Marques, M. T. Q.
Moreno, A.
Barnwell, J. W.
Galinski, M. R.
Ferreira, J. Oliveira
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima Junior, J. C.
Banic, D. M.
Tran, T. M.
Meyer, V. S. E.
Simone, S. G. de
Santos, F.
Porto, L. C. S.
Marques, M. T. Q.
Moreno, A.
Barnwell, J. W.
Galinski, M. R.
Ferreira, J. Oliveira
dc.subject.other.pt_BR.fl_str_mv Plasmodium vivax
Epitopos de Linfócito T
topic Plasmodium vivax
Epitopos de Linfócito T
Plasmodium vivax
Merozoite Surface Protein-9;
promiscuous T-cell epitopes
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Merozoite Surface Protein-9;
promiscuous T-cell epitopes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2017-09-19T15:14:40Z
dc.date.available.fl_str_mv 2017-09-19T15:14:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA JUNIOR, J. C. et al. Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon. Vaccine, v.28, n.18, p.3185-3191, Apr. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/21059
dc.identifier.issn.pt_BR.fl_str_mv 0264-410X
dc.identifier.eissn.none.fl_str_mv 1873-2518
identifier_str_mv LIMA JUNIOR, J. C. et al. Promiscuous T-cell epitopes of Plasmodium Merozoite Surface Protein 9 (PvMSP9) induces IFN-γ and IL-4 responses in individuals naturally exposed to malaria in the Brazilian Amazon. Vaccine, v.28, n.18, p.3185-3191, Apr. 2010.
0264-410X
1873-2518
url https://www.arca.fiocruz.br/handle/icict/21059
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/21059/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/21059/2/SG_Simone_etal_IOC_2010.pdf
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