Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection

Detalhes bibliográficos
Autor(a) principal: Castro, Erika
Data de Publicação: 2003
Outros Autores: Echeverría, Gloria, Deibis, Leopoldo, González de Salmen, Beatríz, Moreira, Aline Dos Santos, Guimarães, Monick Lindenmeyer, Bastos, Francisco Inácio Pinkusfeld Monteiro, Morgado, Mariza Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/726
Resumo: Summary: The authors assessed HIV-1 variability in two distinct areas of Venezuela (the capital Caracas and Margarita Island) through the analysis of blood specimens and clinical and epidemiologic data of 72 persons. Proviral DNA was evaluated through heteroduplex mobility assay (HMA) based on the envelope region. Additionally, FOK I restriction enzyme digestion assay was performed in all subtype B ED31/33 amplified products to check the presence of the typical Brazilian subtype B GWGR variant. Sequencing and phylogenetic analysis for C2-V3 region of gp120 was performed in selected cases. The vast majority of samples were found to belong to subtype B, with a North American/European RFLP profile. An F subtype HIV-1 based on the env region was identified for the first time in Venezuela. Genetic analyses of the protease and reverse transcriptase fragments of this sample depicted a recombinant B/F genetic profile. The discrimination capacity of HIV-1 subtypes using the primer set ED3/14- ED31/33 among the Caracas samples was found to be higher than for those from Margarita Island. The authors’ results point to relevant differences between the samples of continental and Caribbean regions of Venezuela, requiring further evaluation of larger samples.
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spelling Castro, ErikaEcheverría, GloriaDeibis, LeopoldoGonzález de Salmen, BeatrízMoreira, Aline Dos SantosGuimarães, Monick LindenmeyerBastos, Francisco Inácio Pinkusfeld MonteiroMorgado, Mariza Gonçalves2010-08-23T16:58:43Z2010-11-04T14:19:56Z2010-08-23T16:58:43Z2010-11-04T14:19:56Z2003CASTRO, E. et al. Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection. JAIDS: Journal of Acquired Immune Deficiency Syndromes, Hagerstown, v. 32, n. 3, p. 338–344, Mar. 2003.1525-4135https://www.arca.fiocruz.br/handle/icict/7261077-9450Summary: The authors assessed HIV-1 variability in two distinct areas of Venezuela (the capital Caracas and Margarita Island) through the analysis of blood specimens and clinical and epidemiologic data of 72 persons. Proviral DNA was evaluated through heteroduplex mobility assay (HMA) based on the envelope region. Additionally, FOK I restriction enzyme digestion assay was performed in all subtype B ED31/33 amplified products to check the presence of the typical Brazilian subtype B GWGR variant. Sequencing and phylogenetic analysis for C2-V3 region of gp120 was performed in selected cases. The vast majority of samples were found to belong to subtype B, with a North American/European RFLP profile. An F subtype HIV-1 based on the env region was identified for the first time in Venezuela. Genetic analyses of the protease and reverse transcriptase fragments of this sample depicted a recombinant B/F genetic profile. The discrimination capacity of HIV-1 subtypes using the primer set ED3/14- ED31/33 among the Caracas samples was found to be higher than for those from Margarita Island. The authors’ results point to relevant differences between the samples of continental and Caribbean regions of Venezuela, requiring further evaluation of larger samples.This study was supported by grants of the National Council for Scientific and Technological Research from Venezuela “CONICIT,” PAHO/Venezuela, and the Oswaldo Cruz Institute. Results shown in this report are part of the PhD thesis of Erika Castro in the field of Tropical Medicine from the Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil. Current address for Erika Castro: Instituto de Inmunología, Universidad Central de Venezuela, Ciudad Universitaria, Los Chaguaramos, Caracas, 1050-A, Venezuela; e-mail:ebatenjer@hotmail.com Address correspondence and reprint requests to Mariza G. Morgado, Laboratory of AIDS & Molecular Immunology, Dept. of Immunology, Oswaldo Cruz Institute/Fiocruz–UNAIDS Collaborating Centre, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, CEP 21045–900, RJ, Brazil; e-mail: mmorgado@ioc.fiocruz.brFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil / Non Governmental Organization, CRIATEII, Porlamar, Margarita Island, Venezuela.Immunology Institute, Central University of Venezuela, Caracas, Venezuela.Immunology Institute, Central University of Venezuela, Caracas, Venezuela.STDs and AIDS Program, State Hospital “Luis Ortega,” Porlamar, Margarita Island, Venezuela.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto de Comunicação e Informação Científica e Tecnologia em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.engLippincott Williams & Wilkins, Inc., PhiladelphiaHIV-1 diversity in VenezuelaB/F recombinant virus in VenezuelaMolecular epidemiologyHeteroduplex mobility assayNon-B HIV-1 subtypes in Latin AmericaMolecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTBastos_Molecular epidemiology of HIV_2003.pdf.txtBastos_Molecular epidemiology of HIV_2003.pdf.txtExtracted texttext/plain29951https://www.arca.fiocruz.br/bitstream/icict/726/6/Bastos_Molecular%20epidemiology%20of%20HIV_2003.pdf.txtf63a8c63159187f7059e5b90127c7a9dMD56ORIGINALBastos_Molecular epidemiology of HIV_2003.pdfapplication/pdf373182https://www.arca.fiocruz.br/bitstream/icict/726/2/Bastos_Molecular%20epidemiology%20of%20HIV_2003.pdf24f729ff92a361bcdcf0339c478f3127MD52LICENSElicense.txttext/plain1841https://www.arca.fiocruz.br/bitstream/icict/726/3/license.txt51e3e2d2f4675284de038a3647b35cbfMD53THUMBNAILBastos_Molecular epidemiology of HIV_2003.pdf.jpgBastos_Molecular epidemiology of HIV_2003.pdf.jpgGenerated Thumbnailimage/jpeg1998https://www.arca.fiocruz.br/bitstream/icict/726/5/Bastos_Molecular%20epidemiology%20of%20HIV_2003.pdf.jpg717198393e60452a03a5d74abb3598c1MD55icict/7262023-09-04 11:11:01.891oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:11:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
title Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
spellingShingle Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
Castro, Erika
HIV-1 diversity in Venezuela
B/F recombinant virus in Venezuela
Molecular epidemiology
Heteroduplex mobility assay
Non-B HIV-1 subtypes in Latin America
title_short Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
title_full Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
title_fullStr Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
title_full_unstemmed Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
title_sort Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection
author Castro, Erika
author_facet Castro, Erika
Echeverría, Gloria
Deibis, Leopoldo
González de Salmen, Beatríz
Moreira, Aline Dos Santos
Guimarães, Monick Lindenmeyer
Bastos, Francisco Inácio Pinkusfeld Monteiro
Morgado, Mariza Gonçalves
author_role author
author2 Echeverría, Gloria
Deibis, Leopoldo
González de Salmen, Beatríz
Moreira, Aline Dos Santos
Guimarães, Monick Lindenmeyer
Bastos, Francisco Inácio Pinkusfeld Monteiro
Morgado, Mariza Gonçalves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Castro, Erika
Echeverría, Gloria
Deibis, Leopoldo
González de Salmen, Beatríz
Moreira, Aline Dos Santos
Guimarães, Monick Lindenmeyer
Bastos, Francisco Inácio Pinkusfeld Monteiro
Morgado, Mariza Gonçalves
dc.subject.por.fl_str_mv HIV-1 diversity in Venezuela
B/F recombinant virus in Venezuela
Molecular epidemiology
Heteroduplex mobility assay
Non-B HIV-1 subtypes in Latin America
topic HIV-1 diversity in Venezuela
B/F recombinant virus in Venezuela
Molecular epidemiology
Heteroduplex mobility assay
Non-B HIV-1 subtypes in Latin America
description Summary: The authors assessed HIV-1 variability in two distinct areas of Venezuela (the capital Caracas and Margarita Island) through the analysis of blood specimens and clinical and epidemiologic data of 72 persons. Proviral DNA was evaluated through heteroduplex mobility assay (HMA) based on the envelope region. Additionally, FOK I restriction enzyme digestion assay was performed in all subtype B ED31/33 amplified products to check the presence of the typical Brazilian subtype B GWGR variant. Sequencing and phylogenetic analysis for C2-V3 region of gp120 was performed in selected cases. The vast majority of samples were found to belong to subtype B, with a North American/European RFLP profile. An F subtype HIV-1 based on the env region was identified for the first time in Venezuela. Genetic analyses of the protease and reverse transcriptase fragments of this sample depicted a recombinant B/F genetic profile. The discrimination capacity of HIV-1 subtypes using the primer set ED3/14- ED31/33 among the Caracas samples was found to be higher than for those from Margarita Island. The authors’ results point to relevant differences between the samples of continental and Caribbean regions of Venezuela, requiring further evaluation of larger samples.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2010-08-23T16:58:43Z
2010-11-04T14:19:56Z
dc.date.available.fl_str_mv 2010-08-23T16:58:43Z
2010-11-04T14:19:56Z
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dc.identifier.citation.fl_str_mv CASTRO, E. et al. Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection. JAIDS: Journal of Acquired Immune Deficiency Syndromes, Hagerstown, v. 32, n. 3, p. 338–344, Mar. 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/726
dc.identifier.issn.pt_BR.fl_str_mv 1525-4135
dc.identifier.eissn.pt_BR.fl_str_mv 1077-9450
identifier_str_mv CASTRO, E. et al. Molecular epidemiology of HIV-1 in Venezuela: high prevalence of HIV-1 subtype B and identification of a B/F recombinant infection. JAIDS: Journal of Acquired Immune Deficiency Syndromes, Hagerstown, v. 32, n. 3, p. 338–344, Mar. 2003.
1525-4135
1077-9450
url https://www.arca.fiocruz.br/handle/icict/726
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dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins, Inc., Philadelphia
publisher.none.fl_str_mv Lippincott Williams & Wilkins, Inc., Philadelphia
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