Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FURG (RI FURG) |
Texto Completo: | http://repositorio.furg.br/handle/1/2506 |
Resumo: | Objective: To identify a new circulating recombinant form (CRF) of HIV-1 comprising two circulating subtypes in the southern region in Brazil, subtypes B and C. Methods: A total of 152 HIV-positive patients followed at two hospitals in southern Brazil had their viral pol genes isolated by reverse transcriptase–polymerase chain reaction (PCR) from plasma. PCR products were sequenced and phylogenetically analysed using HIV-1 subtype reference sequences. Six full-length subtype C viruses from Brazil previously described as ‘pure’ strains were included in the analysis. Sequences suggestive of recombination were analysed by bootscanning and phylogenetic analyses of separate fragments. The common ancestry of recombinant strains was evaluated by similarity plot and informative site analyses.Results: HIV-1 subtypes commonly found in Brazil (B, C and F1) were observed. Sixtytwo viruses were initially assigned as subtype C, but 15 viruses clustered in a separate internal clade. Pol from two full-length genomes of subtype C viruses grouped together with those samples. Bootscanning analysis showed that all 17 viruses had the same recombinant structure, with a 240 base pair fragment of subtype B in the middle of the reverse transcriptase pol region. Subtype B assignment of this fragment was confirmed by phylogenetic analyses using different methods of tree inference and cluster robustness tests. Mosaics were shown to have a common ancestry. Conclusion: As CRF_BC represents 11% of the HIV-1 viruses circulating in the southern region of the country, which borders several south American countries, the assessment of its sprea is of pivotal importance to the HIV/AIDS epidemic in Brazil and Latin America. |
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Santos, André Felipe Andrade dosMartins, Tathiana Silva de SouzaSoares, Esmeralda Augusta Jardim MachadoSanabani, SabriMartinez, Ana Maria Barral deSprinz, EduardoSilveira, Jussara MariaSabino, EsterTanuri, AmilcarSoares, Marcelo Alves2012-09-13T17:46:01Z2012-09-13T17:46:01Z2006SANTOS, André Felipe Andrade dos et al. Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil. AIDS, Londres, v. 20, n. 16, p. 2011-2019, 2006. Disonível em:<http://journals.lww.com/aidsonline/Fulltext/2006/10240/Characterization_of_a_new_circulating_recombinant.3.aspx>. Acesso em: 9 set. 2012.0269-9370http://repositorio.furg.br/handle/1/2506Objective: To identify a new circulating recombinant form (CRF) of HIV-1 comprising two circulating subtypes in the southern region in Brazil, subtypes B and C. Methods: A total of 152 HIV-positive patients followed at two hospitals in southern Brazil had their viral pol genes isolated by reverse transcriptase–polymerase chain reaction (PCR) from plasma. PCR products were sequenced and phylogenetically analysed using HIV-1 subtype reference sequences. Six full-length subtype C viruses from Brazil previously described as ‘pure’ strains were included in the analysis. Sequences suggestive of recombination were analysed by bootscanning and phylogenetic analyses of separate fragments. The common ancestry of recombinant strains was evaluated by similarity plot and informative site analyses.Results: HIV-1 subtypes commonly found in Brazil (B, C and F1) were observed. Sixtytwo viruses were initially assigned as subtype C, but 15 viruses clustered in a separate internal clade. Pol from two full-length genomes of subtype C viruses grouped together with those samples. Bootscanning analysis showed that all 17 viruses had the same recombinant structure, with a 240 base pair fragment of subtype B in the middle of the reverse transcriptase pol region. Subtype B assignment of this fragment was confirmed by phylogenetic analyses using different methods of tree inference and cluster robustness tests. Mosaics were shown to have a common ancestry. Conclusion: As CRF_BC represents 11% of the HIV-1 viruses circulating in the southern region of the country, which borders several south American countries, the assessment of its sprea is of pivotal importance to the HIV/AIDS epidemic in Brazil and Latin America.engCirculating recombinant formHIV-1Southern BrazilSubtype CRecombinant virusCharacterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FURG (RI FURG)instname:Universidade Federal do Rio Grande (FURG)instacron:FURGORIGINALCharacterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil.pdfCharacterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil.pdfapplication/pdf417025https://repositorio.furg.br/bitstream/1/2506/1/Characterization%20of%20a%20new%20circulating%20recombinant%20form%20comprising%20HIV-1%20subtypes%20C%20and%20B%20in%20southern%20Brazil.pdf17a15e6fe57933306748b6459c78f04fMD51open accessLICENSElicense.txtlicense.txttext/plain; charset=utf-81678https://repositorio.furg.br/bitstream/1/2506/2/license.txtd3be63d3b3eee02729709361dac69efeMD52open access1/25062019-11-22 10:08:51.802open accessoai:repositorio.furg.br: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ório InstitucionalPUBhttps://repositorio.furg.br/oai/request || http://200.19.254.174/oai/requestopendoar:2019-11-22T13:08:51Repositório Institucional da FURG (RI FURG) - Universidade Federal do Rio Grande (FURG)false |
dc.title.pt_BR.fl_str_mv |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
title |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
spellingShingle |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil Santos, André Felipe Andrade dos Circulating recombinant form HIV-1 Southern Brazil Subtype C Recombinant virus |
title_short |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
title_full |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
title_fullStr |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
title_full_unstemmed |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
title_sort |
Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil |
author |
Santos, André Felipe Andrade dos |
author_facet |
Santos, André Felipe Andrade dos Martins, Tathiana Silva de Souza Soares, Esmeralda Augusta Jardim Machado Sanabani, Sabri Martinez, Ana Maria Barral de Sprinz, Eduardo Silveira, Jussara Maria Sabino, Ester Tanuri, Amilcar Soares, Marcelo Alves |
author_role |
author |
author2 |
Martins, Tathiana Silva de Souza Soares, Esmeralda Augusta Jardim Machado Sanabani, Sabri Martinez, Ana Maria Barral de Sprinz, Eduardo Silveira, Jussara Maria Sabino, Ester Tanuri, Amilcar Soares, Marcelo Alves |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos, André Felipe Andrade dos Martins, Tathiana Silva de Souza Soares, Esmeralda Augusta Jardim Machado Sanabani, Sabri Martinez, Ana Maria Barral de Sprinz, Eduardo Silveira, Jussara Maria Sabino, Ester Tanuri, Amilcar Soares, Marcelo Alves |
dc.subject.por.fl_str_mv |
Circulating recombinant form HIV-1 Southern Brazil Subtype C Recombinant virus |
topic |
Circulating recombinant form HIV-1 Southern Brazil Subtype C Recombinant virus |
description |
Objective: To identify a new circulating recombinant form (CRF) of HIV-1 comprising two circulating subtypes in the southern region in Brazil, subtypes B and C. Methods: A total of 152 HIV-positive patients followed at two hospitals in southern Brazil had their viral pol genes isolated by reverse transcriptase–polymerase chain reaction (PCR) from plasma. PCR products were sequenced and phylogenetically analysed using HIV-1 subtype reference sequences. Six full-length subtype C viruses from Brazil previously described as ‘pure’ strains were included in the analysis. Sequences suggestive of recombination were analysed by bootscanning and phylogenetic analyses of separate fragments. The common ancestry of recombinant strains was evaluated by similarity plot and informative site analyses.Results: HIV-1 subtypes commonly found in Brazil (B, C and F1) were observed. Sixtytwo viruses were initially assigned as subtype C, but 15 viruses clustered in a separate internal clade. Pol from two full-length genomes of subtype C viruses grouped together with those samples. Bootscanning analysis showed that all 17 viruses had the same recombinant structure, with a 240 base pair fragment of subtype B in the middle of the reverse transcriptase pol region. Subtype B assignment of this fragment was confirmed by phylogenetic analyses using different methods of tree inference and cluster robustness tests. Mosaics were shown to have a common ancestry. Conclusion: As CRF_BC represents 11% of the HIV-1 viruses circulating in the southern region of the country, which borders several south American countries, the assessment of its sprea is of pivotal importance to the HIV/AIDS epidemic in Brazil and Latin America. |
publishDate |
2006 |
dc.date.issued.fl_str_mv |
2006 |
dc.date.accessioned.fl_str_mv |
2012-09-13T17:46:01Z |
dc.date.available.fl_str_mv |
2012-09-13T17:46:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SANTOS, André Felipe Andrade dos et al. Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil. AIDS, Londres, v. 20, n. 16, p. 2011-2019, 2006. Disonível em:<http://journals.lww.com/aidsonline/Fulltext/2006/10240/Characterization_of_a_new_circulating_recombinant.3.aspx>. Acesso em: 9 set. 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.furg.br/handle/1/2506 |
dc.identifier.issn.none.fl_str_mv |
0269-9370 |
identifier_str_mv |
SANTOS, André Felipe Andrade dos et al. Characterization of a new circulating recombinant form comprising HIV-1 subtypes C and B in southern Brazil. AIDS, Londres, v. 20, n. 16, p. 2011-2019, 2006. Disonível em:<http://journals.lww.com/aidsonline/Fulltext/2006/10240/Characterization_of_a_new_circulating_recombinant.3.aspx>. Acesso em: 9 set. 2012. 0269-9370 |
url |
http://repositorio.furg.br/handle/1/2506 |
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eng |
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eng |
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openAccess |
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