Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10198 |
Resumo: | University of Miami. Miller School of Medicine. Department of Pathology. Miami, Florida, USA. |
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Martins, Mauricio A.Wilson, Nancy A.Plaskowski, Shari M.Weisgrau, Kim L.Furlott, Jessica R.Bonaldo, Myrna C.Santana, Marlon G. Veloso deRudersdorf, Richard A.Rakasz, Eva G.Keating, Karen D.Chiuchiolo, Maria J.Platak Jr, MichaelAllison, David B.Parks, Christopher L.Galler, RicardoLifson, Jeffrey D.Watkins, David I.2015-05-04T17:07:28Z2015-05-04T17:07:28Z2014MARTINS, Mauricio A. et al. Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239. Journal of Virology, v.88, n.13, p. 7493-7516, 2014.1098-5514https://www.arca.fiocruz.br/handle/icict/1019810.1128/JVI.00601-14engAmerican Society for MicrobiologyHIVHuman and simian immunodeficiency viruses (HIV and SIV)HIVVaccination regimensT-cell responsesHIVCiências BiológicasVaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Miami. Miller School of Medicine. Department of Pathology. Miami, Florida, USA.University of Wisconsin. Department of Medicine. Madison, Wisconsin, USA.University of Wisconsin. Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Wisconsin. Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Wisconsin. Wisconsin National Primate Research Center. Madison, Wisconsin, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.University of Miami. Miller School of Medicine. Department of Pathology. Miami, Florida, USA.University of Wisconsin. Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Wisconsin. Wisconsin National Primate Research Center. Madison, Wisconsin, USA.University of Alabama at Birmingham. Department of Biostatistics. Section on Statistical Genetics. Birmingham, Alabama, USA.International AIDS Vaccine Initiative. AIDS Vaccine Design and Development Laboratory. Brooklyn Army Terminal. Brooklyn, New York, USA.Leidos Biomedical Research. Inc. Frederick National Laboratory. AIDS and Cancer Virus Program. Frederick, Maryland, USA.University of Alabama at Birmingham. Department of Biostatistics. Section on Statistical Genetics. Birmingham, Alabama, USA.International AIDS Vaccine Initiative. AIDS Vaccine Design and Development Laboratory. Brooklyn Army Terminal. Brooklyn, New York, USA.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Leidos Biomedical Research. Inc. Frederick National Laboratory. AIDS and Cancer Virus Program. Frederick, Maryland, USA.University of Miami. Miller School of Medicine. Department of Pathology. Miami, Florida, USA.Broadly targeted cellular immune responses are thought to be important for controlling replication of human and simian immunodeficiency viruses (HIV and SIV). However, eliciting such responses by vaccination is complicated by immunodominance, the preferential targeting of only a few of the many possible epitopes of a given antigen. This phenomenon may be due to the coexpression of dominant and subdominant epitopes by the same antigen-presenting cell and may be overcome by distributing these sequences among several different vaccine constructs. Accordingly, we tested whether vaccinating rhesus macaques with “minigenes” encoding fragments of Gag, Vif, and Nef resulted in broadened cellular responses capable of controlling SIV replication.Wedelivered these minigenes through combinations of recombinant Mycobacterium bovis BCG (rBCG), electroporated recombinant DNA (rDNA) along with an interleukin-12 (IL-12)-expressing plasmid (EP rDNA plus pIL-12), yellow fever vaccine virus 17D (rYF17D), and recombinant adenovirus serotype 5 (rAd5). Although priming with EP rDNA plus pIL-12 increased the breadth of vaccine-induced T-cell responses, this effect was likely due to the improved antigen delivery afforded by electroporation rather than modulation of immunodominance. Indeed, Mamu-A*01 vaccinees mounted CD8 T cells directed against only one subdominant epitope, regardless of the vaccination regimen. After challenge with SIVmac239, vaccine efficacy was limited to a modest reduction in set point in some of the groups and did not correlate with standard T-cell measurements. These findings suggest that broad T-cell responses elicited by conventional vectors may not be sufficient to substantially contain AIDS virus replication.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10198/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmyrna_bonaldoetal_IOC_2014.pdfapplication/pdf6308018https://www.arca.fiocruz.br/bitstream/icict/10198/2/myrna_bonaldoetal_IOC_2014.pdf8d6762258d0dfa74834cd8870c4070d5MD52TEXTmyrna_bonaldoetal_IOC_2014.pdf.txtmyrna_bonaldoetal_IOC_2014.pdf.txtExtracted texttext/plain117694https://www.arca.fiocruz.br/bitstream/icict/10198/3/myrna_bonaldoetal_IOC_2014.pdf.txtb46060d5514cd765bef7bdd6c0fa463fMD53icict/101982022-06-24 12:19:40.776oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T15:19:40Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| title |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| spellingShingle |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 Martins, Mauricio A. HIV Human and simian immunodeficiency viruses (HIV and SIV) HIV Vaccination regimens T-cell responses HIV Ciências Biológicas |
| title_short |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| title_full |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| title_fullStr |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| title_full_unstemmed |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| title_sort |
Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239 |
| author |
Martins, Mauricio A. |
| author_facet |
Martins, Mauricio A. Wilson, Nancy A. Plaskowski, Shari M. Weisgrau, Kim L. Furlott, Jessica R. Bonaldo, Myrna C. Santana, Marlon G. Veloso de Rudersdorf, Richard A. Rakasz, Eva G. Keating, Karen D. Chiuchiolo, Maria J. Platak Jr, Michael Allison, David B. Parks, Christopher L. Galler, Ricardo Lifson, Jeffrey D. Watkins, David I. |
| author_role |
author |
| author2 |
Wilson, Nancy A. Plaskowski, Shari M. Weisgrau, Kim L. Furlott, Jessica R. Bonaldo, Myrna C. Santana, Marlon G. Veloso de Rudersdorf, Richard A. Rakasz, Eva G. Keating, Karen D. Chiuchiolo, Maria J. Platak Jr, Michael Allison, David B. Parks, Christopher L. Galler, Ricardo Lifson, Jeffrey D. Watkins, David I. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Martins, Mauricio A. Wilson, Nancy A. Plaskowski, Shari M. Weisgrau, Kim L. Furlott, Jessica R. Bonaldo, Myrna C. Santana, Marlon G. Veloso de Rudersdorf, Richard A. Rakasz, Eva G. Keating, Karen D. Chiuchiolo, Maria J. Platak Jr, Michael Allison, David B. Parks, Christopher L. Galler, Ricardo Lifson, Jeffrey D. Watkins, David I. |
| dc.subject.other.pt_BR.fl_str_mv |
HIV |
| topic |
HIV Human and simian immunodeficiency viruses (HIV and SIV) HIV Vaccination regimens T-cell responses HIV Ciências Biológicas |
| dc.subject.en.pt_BR.fl_str_mv |
Human and simian immunodeficiency viruses (HIV and SIV) HIV Vaccination regimens T-cell responses |
| dc.subject.es.pt_BR.fl_str_mv |
HIV |
| dc.subject.decs.pt_BR.fl_str_mv |
Ciências Biológicas |
| description |
University of Miami. Miller School of Medicine. Department of Pathology. Miami, Florida, USA. |
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2014 |
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2014 |
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2015-05-04T17:07:28Z |
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2015-05-04T17:07:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MARTINS, Mauricio A. et al. Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239. Journal of Virology, v.88, n.13, p. 7493-7516, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10198 |
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1098-5514 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1128/JVI.00601-14 |
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MARTINS, Mauricio A. et al. Vaccination with gag, vif, and nef Gene Fragments Affords Partial Control of Viral Replication after Mucosal Challenge with SIVmac239. Journal of Virology, v.88, n.13, p. 7493-7516, 2014. 1098-5514 10.1128/JVI.00601-14 |
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eng |
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American Society for Microbiology |
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American Society for Microbiology |
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